A transgenic vector was generated encoding the C-terminal hemagglutinin (HA)-tagged human LRRK2*G2019S mutant gene under control of a tetO promoter. The donating investigator reported that the construct was microinjected into C57BL/6J fertilized oocytes, and founder mice were bred to C57BL/6J mice to establish a colony (see SNP note below). Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. Five markers, on Ch 1, 2, 4, 5, and 11, were found to be segregating, suggesting mice sent to The Jackson Laboratory Repository were on a mixed genetic background.
