This HSA-rtTA/TRE-Cre mutant mouse strain may be useful for generating tissue specific-targeted mutants that would be useful in studying gene function in skeletal muscle, and is especially useful for studying steroid and hormone regulation in skeletal muscle and muscle/motorneuron interactions.
Douglas Ashley Monks, University of Toronto Mississauga
Genetic Background | Generation |
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Allele Type |
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Transgenic (Recombinase-expressing, Inducible, Transactivator) |
These transgenic mice have a tetracycline (doxycycline) inducible Cre-mediated recombination system that is specific for skeletal muscle myocytes. Two transgenic constructs were coinjected to generate this strain. The first transgene contains cre recombinase under the control of the tetO, tetracycline-responsive regulatory element and a second transgenic construct contains the reverse tetracycline-controlled transactivator, rtTA (Tet-On), under the control of the human ACTA1, actin, alpha 1, skeletal muscle promoter. Mice hemizygous for the transgenic inserts are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Cre mRNA expression is detected in mice treated with doxycycline (dox) specifically in limb muscle. In the absence of dox, very weak Cre mRNA expression is detected in skeletal muscle. When crossed with a reporter strain, inducible Cre recombinase activity is restricted to skeletal muscle tissues. Slight Cre recombinase activity is detected in skeletal muscle in the absence of dox. Some expression mosaicism is detected between muscle fibers and muscles.
A transgenic construct containing cre, cre recombinase under the control of the tetO, tetracycline-responsive regulatory element and a second transgenic construct containing rtTA, reverse tetracycline-controlled transactivator under the control of the human ACTA1, actin, alpha 1, skeletal muscle promoter were utilized in this strain. The two transgenic constructs were coinjected into fertilized (C57BL/6 X C3H)F2 mouse eggs. Founder line 102 was subsequently established. Female founder animals were bred to wildtype C57BL/6J mice. The mice were backcrossed to C57BL/6J for 3 generations. Upon arrival at The Jackson Laboratory the mice were crossed to C57BL/6J for at least one generation to establish the colony.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Expressed Gene | rtTA, reverse tetracycline-controlled transactivator, E. coli |
Site of Expression | tetracycline (doxycycline) inducible Cre-mediated recombination system that is specific for skeletal muscle myocytes. |
Allele Name | transgene insertion 102, Douglas Monks |
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Allele Type | Transgenic (Recombinase-expressing, Inducible, Transactivator) |
Allele Synonym(s) | HSA-rtTA/TRE-Cre; TRE-Cre |
Gene Symbol and Name | Tg(ACTA1-rtTA,tetO-cre)102Monk, transgene insertion 102, Douglas Monks |
Gene Synonym(s) | |
Promoter | ACTA1, actin, alpha 1, skeletal muscle, human |
Promoter | tetO, tet operator, |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Expressed Gene | rtTA, reverse tetracycline-controlled transactivator, E. coli |
Site of Expression | tetracycline (doxycycline) inducible Cre-mediated recombination system that is specific for skeletal muscle myocytes. |
Strain of Origin | (C57BL/6 x C3H)F2 |
Chromosome | UN |
Molecular Note | A transgenic construct containing cre recombinase under the control of the tetracycline-responsive regulatory element (tetO) and a second transgenic construct containing rtTA, under the control of the human ACTA1, actin, alpha 1, skeletal muscle promoter were coinjected into fertilized (C57BL/6 X C3H)F2 mouse eggs. Founder line 102 was subsequently established. Female founder animals were bred to wildtype C57BL/6J mice. |
When maintaining a live colony, transgene carrier mice may be bred with wildtype (noncarrier) mice from the colony. The Donating Investigator has not attempted to make the strain homozygous.
When using the HSA-rtTA/TRE-Cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #012433 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(ACTA1-rtTA,tetO-cre)102Monk |
Frozen Mouse Embryo | B6;C3-Tg(ACTA1-rtTA tetO-cre)102Monk/J | $2595.00 |
Frozen Mouse Embryo | B6;C3-Tg(ACTA1-rtTA tetO-cre)102Monk/J | $2595.00 |
Frozen Mouse Embryo | B6;C3-Tg(ACTA1-rtTA tetO-cre)102Monk/J | $3373.50 |
Frozen Mouse Embryo | B6;C3-Tg(ACTA1-rtTA tetO-cre)102Monk/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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