NOD.B10Sn-Idd5^C57Bl/10SnJ/R193MrkTacJ

Stock number: 012392
Product name: NOD.B10-Idd5, R193
Research areas: Diabetes and Obesity Research, Immunology, Inflammation and Autoimmunity Research

Description

This strain is one of a set of 9 NOD.B10 congenic Idd5 strains (Stock No. 008893, 008894, 004344, 012391, 012394, 012393, 012395, and 012396 that resulted by crossing segments of C57BL/10SnJ derived chromosome 1 into the NOD/MrkTac background. These strains may be useful to better understand the variation of diabetic resistance conferred by Idd loci; for studying long-range chromosome remodeling and may be useful for identifying diabetes susceptible candidate genes within the Idd5 subloci.

Detailed description

This NOD/MrkTac congenic strain, commonly referred to as NOD.B10-Idd5R193, line 2574 contains a 16.2Mb genomic segment from the diabetes resistant strain C57BL/10SnJ (Stock No. 000666) introgressed into chromosome 1 and is defined within markers D1Mit124 (57.7Mb) and D1Mit180 (73.9Mb). The C57BL/10SnJ genomic segment includes Idd5.1 and recently identified Idd5.3. The major candidate gene for Idd5.1 is Ctla4. The Idd5.3 is defined to a 3.55Mb region. Within this region there are 11 annotated genes; including possible candidate genes IKAROS family zinc finger 2 (Ikzf2) and acyl-Coenzyme A dehydrogenase, long-chain (Acadl) Diabetes incidence is significantly protected among 7-month old females. Diabetes incidence among 7-month old females is reported to be 47%, compared with more than 80% in NOD controls or 62% in NOD.B10-Idd5.1 (Stock No. 012391) (Hunter, et al. 2007).

This strain may be useful for positional mapping and identifying candidate genes within the Idd5.3 region. This strain is useful for understanding the variation in diabetic resistance conferred by Idd loci, in particular the epistatic interactions of Idd5.1 alleles with alleles at Idd5.3

Development

This strain was produced by mating mice from NOD/MrkTac (NOD) to C57BL/10SnJ followed by 15 additional crosses to NOD with selection for chromosome 1, Idd5 markers. Siblings carrying the C57BL/10SnJ derived segments denoted by markers D1Mit124 (57.7Mb) through D1Mit180 (73.9Mb) were intercrossed to make homozygous. 5kSNP Chip Data, based on NCBI, build 37, identifies no C57BL/10SnJ genetic contamination of other chromosomes. The Type 1 Diabetes Resource received this strain in 2010.

Allele

Symbol: Idd5.2^C57BL/10SnJ
Name: C57BL/10SnJ
MGI accession ID: MGI:3036879
Generation method: QTL
Marker symbol: Idd5.2
Marker name: insulin dependent diabetes susceptibility 5.2
Chromosome: 1
Strain of origin: C57BL/10SnJ
Molecular note: This allele confers resistance to insulin dependent diabetes compared to NOD/MrkTac.

Allele

Symbol: Idd5.1^C57BL/10SnJ
Name: C57BL/10SnJ
MGI accession ID: MGI:3036875
Generation method: QTL
Marker symbol: Idd5.1
Marker name: insulin dependent diabetes susceptibility 5.1
Chromosome: 1
Strain of origin: C57BL/10SnJ
Molecular note: This allele confers resistance to insulin dependent diabetes compared to NOD/MrkTac.

Allele

Symbol: Idd5^C57BL/10SnJ
Name: C57BL/10SnJ
MGI accession ID: MGI:3036897
Generation method: QTL
Marker symbol: Idd5
Marker name: insulin dependent diabetes susceptibility 5
Chromosome: 1
Strain of origin: C57BL/10SnJ
Molecular note: This allele confers resistance to insulin dependent diabetes compared to NOD/MrkTac.
General note: Idd3 and Idd5 appear to interact additively. C57BL/6-derived alleles at Idd3 in combination with C57BL/10SnJ-derived alleles at Idd5 partially reverses autoimmune exocrinopathy markers on an NOD genetic background.