NOD.B10Sn-Idd5.1^C57BL/10SnJ/R46MrkTacJ

Stock number: 012391
Product name: NOD.B10-Idd5.1, R2193
Research areas: Diabetes and Obesity Research, Immunology, Inflammation and Autoimmunity Research

Description

This strain is one of a set of eight NOD.B10 congenic Idd5 strains (Stock No. 008893, 008894, 004344,012392, 012393, 012394, and 012395 that resulted by crossing segments of C57BL/10SnJ derived chromosome 1 into the NOD/MrkTac background. These strains may be useful to better understand the variation of diabetic resistance conferred by Idd loci; for studying long-range chromosome remodeling and may be useful for identifying diabetes susceptible candidate genes within the Idd5 subloci.

Detailed description

This NOD congenic strain, commonly referred to as NOD.B10-Idd5R46, line 2193, carries a diabetes resistant C57BL/10SnJ (Stock No. 000666) chromosome 1 segment. The B10 introgressed segment is defined within D1Mit74 (57.6Mb) through AL671560 (SNP marker at approximately 62Mb) and includes the insulin dependent diabetes susceptibility (Idd)5.1; including candidate gene Ctla4. Diabetes incidence is modest but significant among 7 month old female mice of this strain, which is reported at 62%, compared with more than 80% in NOD controls (Hunter, et al. 2007).

This strain may be useful to understand the epistatic interactions of Idd5.1 alleles with other Idd loci.

Development

This NOD/MrkTac congenic stock contains a genomic segment, D1Mit74 (57.6Mb) through AL671560 (SNP marker at approximately 62Mb), from C57BL/10SnJ (B10) introgressed into chromosome 1. The B10 genomic segment includes Idd5.1. The major candidate gene for Idd5.1 is Ctla4. This strain was produced by mating mice from NOD/MrkTac (NOD) to B10 followed by 15 additional crosses to NOD with selection for chromosome 1, Idd5 markers. Siblings carrying the B10 derived segments denoted by markers D1Mit74 through AL671560 were intercrossed to make homozygous. 5kSNP Chip Data, based on NCBI, build 37, identifies no B10 genetic contamination of other chromosomes. The Type 1 Diabetes Resource received this strain in 2010.

Allele

Symbol: Idd5.1^C57BL/10SnJ
Name: C57BL/10SnJ
MGI accession ID: MGI:3036875
Generation method: QTL
Marker symbol: Idd5.1
Marker name: insulin dependent diabetes susceptibility 5.1
Chromosome: 1
Strain of origin: C57BL/10SnJ
Molecular note: This allele confers resistance to insulin dependent diabetes compared to NOD/MrkTac.