These transgenic mice express Ppargc1b (peroxisome proliferative activated receptor, gamma, coactivator 1 beta) regulated by the tetracycline operator (tetO; also called tetracycline-responsive element (TRE, TetRE) or tet-operator). When mated to a mutant strain expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA), tissue Ppargc1b expression may be regulated with the tetracycline analog doxycycline (dox) in the double mutant offspring. This "high expressor" strain may be useful in studies further characterizing the signaling role of the transgene as it pertains to physiology and disease, particularly mitochondrial function.
Daniel Kelly, Burnham Institute for Medical Research
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inducible, Inserted expressed sequence) |
These transgenic mice express Ppargc1b (peroxisome proliferative activated receptor, gamma, coactivator 1 beta) regulated by the tetracycline operator (tetO; also called tetracycline-responsive element (TRE, TetRE) or tet-operator). When mated to a mutant strain expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA), tissue Ppargc1b expression may be regulated with the tetracycline analog doxycycline (dox) in the double mutant offspring. This "high expressor" strain may be useful in studies further characterizing the signaling role of the transgene as it pertains to physiology and disease, particularly mitochondrial function.
Mouse Ppargc1b (peroxisome proliferative activated receptor, gamma, coactivator 1 beta) was placed under the control of the tetracycline operator (tetO). The transgenic vector was introduced to FVB embryos and maintained on an FVB background by the donating laboratory. This is the "high expressor" line number 7.
Expressed Gene | Ppargc1b, peroxisome proliferative activated receptor, gamma, coactivator 1 beta, mouse, laboratory |
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Site of Expression |
Allele Name | transgene insertion 7, Daniel P Kelly |
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Allele Type | Transgenic (Inducible, Inserted expressed sequence) |
Allele Synonym(s) | TRE-PGC-1 beta |
Gene Symbol and Name | Tg(tetO-Ppargc1b)7Dpk, transgene insertion 7, Daniel P Kelly |
Gene Synonym(s) | |
Promoter | tetO, tet operator, |
Expressed Gene | Ppargc1b, peroxisome proliferative activated receptor, gamma, coactivator 1 beta, mouse, laboratory |
Strain of Origin | FVB |
Chromosome | UN |
Molecular Note | Mouse Ppargc1b (peroxisome proliferative activated receptor, gamma, coactivator 1 beta) was placed under the control of the tetracycline operator (tetO). This is the "high expressor" line number 7. |
Mutations Made By | Teresa Leone, Burnham Institute for Medical Research |
When maintained as a live colony, hemizygous males may be bred with wildtype females. The donating laboratory reports having difficulty preserving expression when using a female hemizygote in the cross (possibly an imprinting phenomenon).
When using the FVB-Tg(tetO-Ppargc1b)7Dpk/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #012385 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(tetO-Ppargc1b)7Dpk |
Frozen Mouse Embryo | FVB-Tg(tetO-Ppargc1b)7Dpk/J | $2595.00 |
Frozen Mouse Embryo | FVB-Tg(tetO-Ppargc1b)7Dpk/J | $2595.00 |
Frozen Mouse Embryo | FVB-Tg(tetO-Ppargc1b)7Dpk/J | $3373.50 |
Frozen Mouse Embryo | FVB-Tg(tetO-Ppargc1b)7Dpk/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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