FVB-Tg(tetO-Ppargc1b)7Dpk/J

Stock number: 012385
Research areas: Research Tools

Description

These transgenic mice express Ppargc1b (peroxisome proliferative activated receptor, gamma, coactivator 1 beta) regulated by the tetracycline operator (tetO; also called tetracycline-responsive element (TRE, TetRE) or tet-operator). When mated to a mutant strain expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA), tissue Ppargc1b expression may be regulated with the tetracycline analog doxycycline (dox) in the double mutant offspring. This "high expressor" strain may be useful in studies further characterizing the signaling role of the transgene as it pertains to physiology and disease, particularly mitochondrial function.

Detailed description

These transgenic mice express Ppargc1b (peroxisome proliferative activated receptor, gamma, coactivator 1 beta) regulated by the tetracycline operator (tetO; also called tetracycline-responsive element (TRE, TetRE) or tet-operator). When mated to a mutant strain expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA), tissue Ppargc1b expression may be regulated with the tetracycline analog doxycycline (dox) in the double mutant offspring. This "high expressor" strain may be useful in studies further characterizing the signaling role of the transgene as it pertains to physiology and disease, particularly mitochondrial function.

Development

Mouse Ppargc1b (peroxisome proliferative activated receptor, gamma, coactivator 1 beta) was placed under the control of the tetracycline operator (tetO). The transgenic vector was introduced to FVB embryos and maintained on an FVB background by the donating laboratory. This is the "high expressor" line number 7.

Allele

Symbol: Tg(tetO-Ppargc1b)7Dpk
Name: transgene insertion 7, Daniel P Kelly
MGI accession ID: MGI:4429484
Generation method: Transgenic
Attributes: Inducible; Inserted expressed sequence
Marker symbol: Tg(tetO-Ppargc1b)7Dpk
Marker name: transgene insertion 7, Daniel P Kelly
Chromosome: UN
Strain of origin: FVB
Expressed genes: Ppargc1b,peroxisome proliferative activated receptor, gamma, coactivator 1 beta,mouse, laboratory
Molecular note: Mouse Ppargc1b (peroxisome proliferative activated receptor, gamma, coactivator 1 beta) was placed under the control of the tetracycline operator (tetO). This is the "high expressor" line number 7.