Mean respiration rates of mitochondria of homozygous Ppargc1b (peroxisome proliferative activated receptor, gamma, coactivator 1 beta) targeted mutant mice are modestly decreased. When combined with a Ppargc1a (peroxisome proliferative activated receptor, gamma, coactivator 1 alpha) targeted mutation (see Stock No. 009662), compound mutant animals die shortly after birth with small hearts, bradycardia, intermittent heart block and a markedly reduced cardiac output. Compound mutants also exhibit a severe abnormality in the function and density of mitochondria in heart and brown adipose tissue. This strain may be useful in studies of cardiac development and mitochondrial function.
Daniel Kelly, Burnham Institute for Medical Research
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Ppargc1b | peroxisome proliferative activated receptor, gamma, coactivator 1 beta |
Mean state 3 respiration rates of mitochondria isolated from hind limb muscle of homozygous Ppargc1b (peroxisome proliferative activated receptor, gamma, coactivator 1 beta) targeted mutant mice are modestly decreased. Electron microscopic analysis of brown adipose tissue does not reveal any significant abnormalities in mitochondrial volume, density, or ultrastructure. When subjected to short-term cold exposure, homozygotes are unable to maintain core body temperature to the same extent as wildtype controls. Homozygotes are born at slightly reduced rates from heterozygous crosses (reported as ~17%), but appear normal. No significant phenotype is observed under non-stressed conditions, and heart function is normal.
When combined with a Ppargc1a (peroxisome proliferative activated receptor, gamma, coactivator 1 alpha) targeted mutation (see Stock No. 009662), compound mutant animals die shortly after birth with small hearts, bradycardia, intermittent heart block and a markedly reduced cardiac output. Compound mutants also exhibit a severe abnormality in the function and density of mitochondria in heart and brown adipose tissue.
This strain may be useful in studies of cardiac development and mitochondrial function.
A loxP-flanked neomycin resistance cassette was introduced into intron 3 and a third loxP site was placed in intron 6 in 129X1Sv/J-derived SCC10 embryonic stem (ES) cells. Resultant female mice were crossed with a male EIIa-cre animal to excise exons 4-6. This strain was backcrossed to C57BL/6 for 12 generations by the donating laboratory.
Allele Name | targeted mutation 1.1, Daniel P Kelly |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | PGC-1beta- |
Gene Symbol and Name | Ppargc1b, peroxisome proliferative activated receptor, gamma, coactivator 1 beta |
Gene Synonym(s) | |
Strain of Origin | 129X1/SvJ |
Chromosome | 18 |
Molecular Note | Germ line, cre-mediated recombination was used to remove exons 4 through 6. Removal of these exons results in a frame shift and a premature STOP codon in exon 7. The absence of protein product was confirmed by western blot analysis on heart extracts. |
Mutations Made By | Teresa Leone, Burnham Institute for Medical Research |
When maintained as a live colony, homozygotes or heterozygotes may be bred. Homozygotes are born at slightly reduced rates from heterozygous crosses (reported as ~17%).
When using the B6.129X1-Ppargc1btm1.1Dpk/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #012378 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Ppargc1b<tm1.1Dpk> |
Frozen Mouse Embryo | B6.129X1-Ppargc1b<tm1.1Dpk>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129X1-Ppargc1b<tm1.1Dpk>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129X1-Ppargc1b<tm1.1Dpk>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129X1-Ppargc1b<tm1.1Dpk>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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