JAX Mouse Strain Datasheet - 012377

B6.129S-Cyp19a1^tm1.1Shah/J

Stock No:: 012377

Cryo Recovery

Overview

These mutant mice possess a reporter cassette, IRES-PLAP-IRES-nlacZ, downstream of the stop codon in the 3' UTR of the targeted locus. These mice may be useful for visualizing aromatase-positive cells to define where testosterone may be converted into estrogen in the mouse brain.

Donating Investigator

Nirao Shah, University of California, San Francisco

Genetic overview

Genetic Background	Generation

Cyp19a1^tm1.1Shah

Allele Type	Gene Symbol	Gene Name
Targeted (Reporter)	Cyp19a1	cytochrome P450, family 19, subfamily a, polypeptide 1

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Research Applications

Neurobiology Research
Research Tools

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Base Price

Starting at:

$2,595.00 Domestic price Cryo Recovery

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Details

Detailed Description

Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These mutant mice possess a reporter cassette, IRES-PLAP-IRES-nlacZ, downstream of the stop codon in the 3' UTR of the targeted locus. Nuclear β-galactosidase (β-gal) labels the nuclei of cells expressing aromatase, whereas human placental alkaline phosphatase (ALPP or PLAP) labels the cell membrane of such cells. These mice may be useful for visualizing aromatase-positive cells to define where testosterone may be converted into estrogen in the mouse brain.

Development

A targeting vector was designed with an internal ribosome entry site (IRES), a human placental alkaline phosphatase gene (ALPP or PLAP), a second IRES, and a nuclear targeted β-galactosidase (lacZ). This vector was introduced after the stop codon of the aromatase gene via electroporation into 129S/SvEv-derived MM13 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric males were bred to C57BL/6J females. This line was backcrossed nine generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.

Expression Data

Expressed Gene	ALPP, alkaline phosphatase, placental, human
Expressed Gene	lacZ, beta-galactosidase, E. coli
Site of Expression	lacZ is expressed in the nuclei of cells expressing aromatase, whereas human placental alkaline phosphatase (ALPP or PLAP) labels the cell membrane of such cells.

Control Suggestions

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Wu MV; Manoli DS; Fraser EJ; Coats JK; Tollkuhn J; Honda S; Harada N; Shah NM. 2009. Estrogen masculinizes neural pathways and sex-specific behaviors. Cell 139(1):61-72. PubMed: 19804754 MGI: J:156012

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Genetics

Cyp19a1^tm1.1Shah

Allele Symbol: Cyp19a1^tm1.1Shah

Allele Name	targeted mutation 1.1, Nirao Shah
Allele Type	Targeted (Reporter)
Allele Synonym(s)	aro^IPIN; aromatase-IPIN
Gene Symbol and Name	Cyp19a1, cytochrome P450, family 19, subfamily a, polypeptide 1
Gene Synonym(s)	ARO; ARO1; Ar; ArKO; Aromatase; CPV1; CYAR; CYP19; CYPXIX; Cyp19; Cyp19; Cyp19a; Int-5; Int-5; Int5; Int5; P-450AROM; aromatase; cytochrome P450, 19, aromatase; mammary tumor integration site 5; p450arom
Expressed Gene	ALPP, alkaline phosphatase, placental, human
Expressed Gene	lacZ, beta-galactosidase, E. coli
Site of Expression	lacZ is expressed in the nuclei of cells expressing aromatase, whereas human placental alkaline phosphatase (ALPP or PLAP) labels the cell membrane of such cells.
Strain of Origin	129S/SvEv
Chromosome	9
Molecular Note	This allele contains an internal ribosome entry site (IRES), a human placental alkaline phosphatase gene, a second IRES, and a nuclear-targeted E. coli nlacZ gene inserted three base pairs 3' of the stop codon of the aromatase gene. The targeting vector also contained a self-excising neomycin resistance cassette (ACN) upstream of the IRES-ALPP-IRES nlacZ. Upon passage through the male germline ACN was deleted, leaving a single loxP site and the IRES-ALPP-IRES-nlacZ construct.
Mutations Made By	Nirao Shah, University of California, San Francisco

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Neurobiology Research
- lacZ expression in neural tissue
Research Tools
- lacZ Expression
- Genetics Research
  - Tissue/Cell Markers
  - Tissue/Cell Markers: neurons
- Neurobiology Research
  - cell marker

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Cyp19a1^tm1.1Shah/Cyp19a1^tm1.1Shah
involves: 129S/SvEv * C57BL/6

normal phenotype

no abnormal phenotype detected

The following phenotype relates to a compound genotype created using this strain

Genotype: Il1r1^tm1Quan/Il1r1^tm1Quan Lyz2^tm1(cre)Ifo/Lyz2^tm1(cre)Ifo
involves: 129P2/OlaHsd * C57BL/6

hematopoietic system phenotype

abnormal microglial cell activation
- microglia are only activated (deramified) in hippocampus region following IL1beta injection rather than throughout the brain parenchyma

immune system phenotype

abnormal inflammatory response
- intracerebroventricular IL1beta injection does not result in leukocyte infiltration to the brain parenchyma in contrast to wild-type controls

abnormal microglial cell activation
- microglia are only activated (deramified) in hippocampus region following IL1beta injection rather than throughout the brain parenchyma

nervous system phenotype

abnormal microglial cell activation
- microglia are only activated (deramified) in hippocampus region following IL1beta injection rather than throughout the brain parenchyma

References

Wu MV; Manoli DS; Fraser EJ; Coats JK; Tollkuhn J; Honda S; Harada N; Shah NM. 2009. Estrogen masculinizes neural pathways and sex-specific behaviors. Cell 139(1):61-72. PubMed: 19804754 MGI: J:156012

Additional - Cyp19a1^tm1.1Shah related

Bunting M; Bernstein KE; Greer JM; Capecchi MR; Thomas KR. 1999. Targeting genes for self-excision in the germ line. Genes Dev 13(12):1524-8. PubMed: 10385621 MGI: J:79620
Unger EK; Burke KJ Jr; Yang CF; Bender KJ; Fuller PM; Shah NM. 2015. Medial amygdalar aromatase neurons regulate aggression in both sexes. Cell Rep 10(4):453-62. PubMed: 25620703 MGI: J:220075

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Please inquire about possible genotypes.

Progeny testing is not required

The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks.

The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Cyp19a1tm1.1Shah

Allele Symbol: Cyp19a1tm1.1Shah

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Cyp19a1tm1.1Shah/Cyp19a1tm1.1Shahinvolves: 129S/SvEv * C57BL/6

normal phenotype

Genotype: Il1r1tm1Quan/Il1r1tm1Quan Lyz2tm1(cre)Ifo/Lyz2tm1(cre)Ifoinvolves: 129P2/OlaHsd * C57BL/6

hematopoietic system phenotype

immune system phenotype

nervous system phenotype

References

Additional - Cyp19a1tm1.1Shah related

Genotyping Protocols

Breeding Considerations

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Progeny testing is not required

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Cyp19a1^tm1.1Shah

Allele Symbol: Cyp19a1^tm1.1Shah

Genotype: Cyp19a1^tm1.1Shah/Cyp19a1^tm1.1Shah
involves: 129S/SvEv * C57BL/6

Genotype: Il1r1^tm1Quan/Il1r1^tm1Quan Lyz2^tm1(cre)Ifo/Lyz2^tm1(cre)Ifo
involves: 129P2/OlaHsd * C57BL/6

Additional - Cyp19a1^tm1.1Shah related