These mutant mice possess a reporter cassette, IRES-PLAP-IRES-nlacZ, downstream of the stop codon in the 3' UTR of the targeted locus. These mice may be useful for visualizing aromatase-positive cells to define where testosterone may be converted into estrogen in the mouse brain.
Nirao Shah, Stanford University
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter) | Cyp19a1 | cytochrome P450, family 19, subfamily a, polypeptide 1 |
Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These mutant mice possess a reporter cassette, IRES-PLAP-IRES-nlacZ, downstream of the stop codon in the 3' UTR of the targeted locus. Nuclear β-galactosidase (β-gal) labels the nuclei of cells expressing aromatase, whereas human placental alkaline phosphatase (ALPP or PLAP) labels the cell membrane of such cells. These mice may be useful for visualizing aromatase-positive cells to define where testosterone may be converted into estrogen in the mouse brain.
A targeting vector was designed with an internal ribosome entry site (IRES), a human placental alkaline phosphatase gene (ALPP or PLAP), a second IRES, and a nuclear targeted β-galactosidase (lacZ). This vector was introduced after the stop codon of the aromatase gene via electroporation into 129S/SvEv-derived MM13 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric males were bred to C57BL/6J females. This line was backcrossed nine generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.
Expressed Gene | ALPP, alkaline phosphatase, placental, human |
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Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | lacZ is expressed in the nuclei of cells expressing aromatase, whereas human placental alkaline phosphatase (ALPP or PLAP) labels the cell membrane of such cells. |
Allele Name | targeted mutation 1.1, Nirao Shah |
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Allele Type | Targeted (Reporter) |
Allele Synonym(s) | aroIPIN; aromatase-IPIN |
Gene Symbol and Name | Cyp19a1, cytochrome P450, family 19, subfamily a, polypeptide 1 |
Gene Synonym(s) | |
Expressed Gene | ALPP, alkaline phosphatase, placental, human |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | lacZ is expressed in the nuclei of cells expressing aromatase, whereas human placental alkaline phosphatase (ALPP or PLAP) labels the cell membrane of such cells. |
Strain of Origin | 129S/SvEv |
Chromosome | 9 |
Molecular Note | This allele contains an internal ribosome entry site (IRES), a human placental alkaline phosphatase gene, a second IRES, and a nuclear-targeted E. coli nlacZ gene inserted three base pairs 3' of the stop codon of the aromatase gene. The targeting vector also contained a self-excising neomycin resistance cassette (ACN) upstream of the IRES-ALPP-IRES nlacZ. Upon passage through the male germline ACN was deleted, leaving a single loxP site and the IRES-ALPP-IRES-nlacZ construct. |
Mutations Made By | Nirao Shah, Stanford University |
When maintaining a live colony, homozygous mice may be bred together.
When using the B6.129S-Cyp19a1tm1.1Shah/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #012377 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wildtype for Cyp19a1<tm1.1Shah> |
Frozen Mouse Embryo | B6.129S-Cyp19a1<tm1.1Shah>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S-Cyp19a1<tm1.1Shah>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S-Cyp19a1<tm1.1Shah>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129S-Cyp19a1<tm1.1Shah>/J Frozen Embryo | $3373.50 |
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