JAX Mouse Strain Datasheet - 012377

B6.129S-Cyp19a1^tm1.1Shah/J

Stock No:: 012377

Cryo Recovery

Overview

These mutant mice possess a reporter cassette, IRES-PLAP-IRES-nlacZ, downstream of the stop codon in the 3' UTR of the targeted locus. These mice may be useful for visualizing aromatase-positive cells to define where testosterone may be converted into estrogen in the mouse brain.

Donating Investigator

Nirao Shah, University of California, San Francisco

Genetic overview

Genetic Background	Generation

Cyp19a1^tm1.1Shah

Allele Type	Gene Symbol	Gene Name
Targeted (Reporter)	Cyp19a1	cytochrome P450, family 19, subfamily a, polypeptide 1

View Genetics

Research Applications

Neurobiology Research
Research Tools

View All Research Applications

Base Price

Starting at:

$2,595.00 Domestic price Cryo Recovery

View Price List

Details

Detailed Description

Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These mutant mice possess a reporter cassette, IRES-PLAP-IRES-nlacZ, downstream of the stop codon in the 3' UTR of the targeted locus. Nuclear β-galactosidase (β-gal) labels the nuclei of cells expressing aromatase, whereas human placental alkaline phosphatase (ALPP or PLAP) labels the cell membrane of such cells. These mice may be useful for visualizing aromatase-positive cells to define where testosterone may be converted into estrogen in the mouse brain.

Development

A targeting vector was designed with an internal ribosome entry site (IRES), a human placental alkaline phosphatase gene (ALPP or PLAP), a second IRES, and a nuclear targeted β-galactosidase (lacZ). This vector was introduced after the stop codon of the aromatase gene via electroporation into 129S/SvEv-derived MM13 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric males were bred to C57BL/6J females. This line was backcrossed nine generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.

Expression Data

Expressed Gene	ALPP, alkaline phosphatase, placental, human
Expressed Gene	lacZ, beta-galactosidase, E. coli
Site of Expression	lacZ is expressed in the nuclei of cells expressing aromatase, whereas human placental alkaline phosphatase (ALPP or PLAP) labels the cell membrane of such cells.

Control Suggestions

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Wu MV; Manoli DS; Fraser EJ; Coats JK; Tollkuhn J; Honda S; Harada N; Shah NM. 2009. Estrogen masculinizes neural pathways and sex-specific behaviors. Cell 139(1):61-72. PubMed: 19804754 MGI: J:156012

View All References

Genetics

Cyp19a1^tm1.1Shah

Allele Symbol: Cyp19a1^tm1.1Shah

Allele Name	targeted mutation 1.1, Nirao Shah
Allele Type	Targeted (Reporter)
Allele Synonym(s)	aro^IPIN; aromatase-IPIN
Gene Symbol and Name	Cyp19a1, cytochrome P450, family 19, subfamily a, polypeptide 1
Gene Synonym(s)	ARO; ARO1; Ar; ArKO; Aromatase; CPV1; CYAR; CYP19; CYPXIX; Cyp19; Cyp19; Cyp19a; Int-5; Int-5; Int5; Int5; P-450AROM; aromatase; cytochrome P450, 19, aromatase; mammary tumor integration site 5; p450arom
Expressed Gene	ALPP, alkaline phosphatase, placental, human
Expressed Gene	lacZ, beta-galactosidase, E. coli
Site of Expression	lacZ is expressed in the nuclei of cells expressing aromatase, whereas human placental alkaline phosphatase (ALPP or PLAP) labels the cell membrane of such cells.
Strain of Origin	129S/SvEv
Chromosome	9
Molecular Note	This allele contains an internal ribosome entry site (IRES), a human placental alkaline phosphatase gene, a second IRES, and a nuclear-targeted E. coli nlacZ gene inserted three base pairs 3' of the stop codon of the aromatase gene. The targeting vector also contained a self-excising neomycin resistance cassette (ACN) upstream of the IRES-ALPP-IRES nlacZ. Upon passage through the male germline ACN was deleted, leaving a single loxP site and the IRES-ALPP-IRES-nlacZ construct.
Mutations Made By	Nirao Shah, University of California, San Francisco

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Neurobiology Research
- lacZ expression in neural tissue
Research Tools
- lacZ Expression
- Genetics Research
  - Tissue/Cell Markers
  - Tissue/Cell Markers: neurons
- Neurobiology Research
  - cell marker

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Cyp19a1^tm1.1Shah/Cyp19a1^tm1.1Shah
involves: 129S/SvEv * C57BL/6

normal phenotype

no abnormal phenotype detected

The following phenotype relates to a compound genotype created using this strain

Genotype: Il1r1^tm1Quan/Il1r1^tm1Quan Lyz2^tm1(cre)Ifo/Lyz2^tm1(cre)Ifo
involves: 129P2/OlaHsd * C57BL/6

hematopoietic system phenotype

abnormal microglial cell activation
- microglia are only activated (deramified) in hippocampus region following IL1beta injection rather than throughout the brain parenchyma

immune system phenotype

abnormal inflammatory response
- intracerebroventricular IL1beta injection does not result in leukocyte infiltration to the brain parenchyma in contrast to wild-type controls

abnormal microglial cell activation
- microglia are only activated (deramified) in hippocampus region following IL1beta injection rather than throughout the brain parenchyma

nervous system phenotype

abnormal microglial cell activation
- microglia are only activated (deramified) in hippocampus region following IL1beta injection rather than throughout the brain parenchyma

References

Wu MV; Manoli DS; Fraser EJ; Coats JK; Tollkuhn J; Honda S; Harada N; Shah NM. 2009. Estrogen masculinizes neural pathways and sex-specific behaviors. Cell 139(1):61-72. PubMed: 19804754 MGI: J:156012

Additional - Cyp19a1^tm1.1Shah related

Bunting M; Bernstein KE; Greer JM; Capecchi MR; Thomas KR. 1999. Targeting genes for self-excision in the germ line. Genes Dev 13(12):1524-8. PubMed: 10385621 MGI: J:79620
Unger EK; Burke KJ Jr; Yang CF; Bender KJ; Fuller PM; Shah NM. 2015. Medial amygdalar aromatase neurons regulate aggression in both sexes. Cell Rep 10(4):453-62. PubMed: 25620703 MGI: J:220075

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous or Wildtype for Cyp19a1<tm1.1Shah>

Progeny testing is not required

The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks.

The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

Terms Of Use

Terms of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

Use of MICE by companies or for-profit entities requires a license prior to shipping.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCT(S)" means biological materials supplied by JACKSON, and their derivatives. "SERVICES" means projects conducted by JACKSON for other parties that may include but are not limited to the use of MICE or PRODUCTS. "RECIPIENT" means each recipient of MICE, PRODUCTS, or SERVICES provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE, PRODUCTS or SERVICES from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON’s prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED "AS IS". JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

Credit for PRODUCTS or SERVICES

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of, PRODUCTS or SERVICES, JACKSON will, at its option, provide credit or replacement for the PRODUCT received or the SERVICES provided; JACKSON makes no other representations and this shall be the exclusive remedy of the purchaser. Please note specific policy for live mice.

Animal Care and Use for SERVICES

Consistent with the requirement for a written understanding regarding animal care and use, the JACKSON Animal Care and Use Committee will review the animal care and use protocol(s) associated with any SERVICES to be performed at JACKSON, and JACKSON shall have ultimate responsibility and authority for the care of animals while on site or in JACKSON custody.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE, PRODUCTS or SERVICES are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or SERVICES shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or SERVICES by JACKSON.

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Cyp19a1tm1.1Shah

Allele Symbol: Cyp19a1tm1.1Shah

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Cyp19a1tm1.1Shah/Cyp19a1tm1.1Shahinvolves: 129S/SvEv * C57BL/6

normal phenotype

Genotype: Il1r1tm1Quan/Il1r1tm1Quan Lyz2tm1(cre)Ifo/Lyz2tm1(cre)Ifoinvolves: 129P2/OlaHsd * C57BL/6

hematopoietic system phenotype

immune system phenotype

nervous system phenotype

References

Additional - Cyp19a1tm1.1Shah related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Progeny testing is not required

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Cyp19a1^tm1.1Shah

Allele Symbol: Cyp19a1^tm1.1Shah

Genotype: Cyp19a1^tm1.1Shah/Cyp19a1^tm1.1Shah
involves: 129S/SvEv * C57BL/6

Genotype: Il1r1^tm1Quan/Il1r1^tm1Quan Lyz2^tm1(cre)Ifo/Lyz2^tm1(cre)Ifo
involves: 129P2/OlaHsd * C57BL/6

Additional - Cyp19a1^tm1.1Shah related