These floxed mutant mice possess loxP sites flanking the coding region of the Junb gene. This strain may be useful for generating conditional mutations in applications related to the study of the role played by Junb in development, inflammation, immune responses and proliferative disease.
Dr. Erwin Wagner, Centro Nacional de Investigaciones Oncológicas
As part of the dimeric transcription factor, AP-1 (activator protein-1), the transcription factor encoded by the jun B proto-oncogene (Junb) is critical during mouse development, regulating apoptosis, differentiation, proliferation, as well as inflammation and tumor progression. These mice possess loxP sites on either side of the coding sequence of the targeted Junb gene: loxP sites inserted into the 5' untranslated region and 161 bp downstream of the translation stop codon. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have the entire coding sequence deleted in the cre-expressing tissues. Removal of the floxed sequence creates a null allele.
When bred to a strain with Cre recombinase expression in early embryos (see Stock No. 003755 for example), this mutant mouse strain may be useful in studies of cell proliferation and myeloproliferative disease.
When bred to a strain with Cre recombinase expression in epithelial cells, to delete Junb expression in the skin, this mutant mouse strain may be useful in studies of wound healing, epithelialization and inflammatory response.
A targeting vector containing a loxP site and a a FRT-site flanked thymidine kinase-neomycin resistance cassette was utilized in the construction of this mutant. This selection cassette was inserted in the 5' untranslated region of the targeted gene, and another loxP site was inserted 161 bp downstream of the translation stop codon, flanking the entire coding region with loxP sites. This construct was electroporated into 129P2/OlaHsd-Hprtb-m3 derived HM-1 embryonic stem (ES) cells which were transiently transfected with a FLP recombinase vector to remove the selection cassette. Correctly targeted ES cells were injected into C57BL/6 blastocysts.
The resulting chimeric animals were tested for germline transmission.
The mice were backcrossed to C57BL/6 for 10 generations.
Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
|Allele Name||targeted mutation 3, Erwin F Wagner|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||junBdelta; junBf; JunBtmAngl|
|Gene Symbol and Name||Junb, jun B proto-oncogene|
|Strain of Origin||129P2/OlaHsd-Hprtb-m3|
|Molecular Note||A floxed and frt-flanked selection cassette containing both a neomycin resistance gene and a thymidine kinase gene was inserted in the 5' untranslated region. An additional loxP site was inserted 161 bp downstream of the translation stop codon. The neomycin and thymidine kinase genes were subsequently deleted from properly targeted ES cells by transient transfection with a flp recombinase expressing vector leaving the coding sequence flanked by loxP sites. Northern blot and Western blot analysis confirmed the absence of both transcripts and protein product.|
When maintaining a live colony, these mice can be bred as homozygotes.
When using the junB flox mouse strain in a publication, please cite the originating article(s) and include JAX stock #012369 in your Materials and Methods section.