These Pbx3C mutant mice possess loxP sites on either side of exon 3 of the targeted gene. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissue(s). These mice may be useful for studying neurodevelopment and locomotion.
Dr. Stephen O'Gorman, Case Western Reserve University
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Pbx3 | pre B cell leukemia homeobox 3 |
These Pbx3C mutant mice possess loxP sites on either side of exon 3 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissue(s). The donating investigator reports that the neo cassette is still present downstream of the floxed exon and it is unknown whether the presence of neo conveys any abnormalities.
A targeting vector was designed to insert a loxP site upstream of exon 3, and a second loxP site followed by a frt-flanked PGK-neo cassette just downstream of exon 3 of the targeted gene. The construct was electroporated into 129S4/SvJae-derived PC3 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a Cre recombinase expression plasmid. ES cells were injected into blastocysts and resulting chimeric males were bred to C57BL/6 females. In the progeny, a subset of mice were found to still harbor the floxed exon 3 (Pbx3C). Mice heterozygous for this conditional Pbx3C allele were bred together to generate homozygotes prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.
Allele Name | targeted mutation 1, Stephen O'Gorman |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Pbx3C |
Gene Symbol and Name | Pbx3, pre B cell leukemia homeobox 3 |
Gene Synonym(s) | |
Strain of Origin | 129S4/SvJae-Tg(Prm-cre)70Og |
Chromosome | 2 |
Molecular Note | A neo cassette with a 5' loxP site was inserted into intron 3 and an additional loxP site was inserted into intron 2. Targeting took place in PC3 ES cells, which are homozygous for Tg(Prm-cre)70Og, a cre transgene active only in haploid spermatids. A minority of the alleles created from this construct exhibited a deficiency of cre-mediated recombination driven by the male-germline specific protamine promoter in male germ cells and transmitted the conditional allele through male chimeric mice. |
Mutations Made By | Dr. Stephen O'Gorman, Case Western Reserve University |
When maintaining a live colony, homozygous mice may be bred together.
When using the B6;129S4-Pbx3tm1Og/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #012356 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Pbx3<tm1Og> |
Frozen Mouse Embryo | B6;129S4-Pbx3<tm1Og>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129S4-Pbx3<tm1Og>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129S4-Pbx3<tm1Og>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129S4-Pbx3<tm1Og>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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