B6;129S4-Pbx3^tm1Og/J

Stock number: 012356
Research areas: Neurobiology Research, Research Tools

Description

These Pbx3C mutant mice possess loxP sites on either side of exon 3 of the targeted gene. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissue(s). These mice may be useful for studying neurodevelopment and locomotion.

Detailed description

These Pbx3C mutant mice possess loxP sites on either side of exon 3 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissue(s). The donating investigator reports that the neo cassette is still present downstream of the floxed exon and it is unknown whether the presence of neo conveys any abnormalities.

Development

A targeting vector was designed to insert a loxP site upstream of exon 3, and a second loxP site followed by a frt-flanked PGK-neo cassette just downstream of exon 3 of the targeted gene. The construct was electroporated into 129S4/SvJae-derived PC3 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a Cre recombinase expression plasmid. ES cells were injected into blastocysts and resulting chimeric males were bred to C57BL/6 females. In the progeny, a subset of mice were found to still harbor the floxed exon 3 (Pbx3C). Mice heterozygous for this conditional Pbx3C allele were bred together to generate homozygotes prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Pbx3^tm1Og
Name: targeted mutation 1, Stephen O'Gorman
MGI accession ID: MGI:3773271
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Pbx3
Marker name: pre B cell leukemia homeobox 3
Chromosome: 2
Strain of origin: 129S4/SvJae-Tg(Prm-cre)70Og
Molecular note: A neo cassette with a 5' loxP site was inserted into intron 3 and an additional loxP site was inserted into intron 2. Targeting took place in PC3 ES cells, which are homozygous for Tg(Prm-cre)70Og, a cre transgene active only in haploid spermatids. A minority of the alleles created from this construct exhibited a deficiency of cre-mediated recombination driven by the male-germline specific protamine promoter in male germ cells and transmitted the conditional allele through male chimeric mice.