NOD.B10Sn-Idd9.3^C57BL/10SnJ/1106MrkTacJ

Stock number: 012311
Product name: NOD.B10-Idd9.3,R1106
Research areas: Diabetes and Obesity Research, Immunology, Inflammation and Autoimmunity Research

Description

This chromosome 4 congenic stock contains the C57BL/10SnJ derived Idd9.3 region introgressed into the NOD/MrkTac background and exhibits significantly reduced diabetes incidence. This strain may be useful for positional mapping of the Idd9.3 loci and studying candidate genes within the Idd9.3 region.

Detailed description

This NOD/MrkTac congenic stock, commonly referred to as NOD.B10-Idd9R35 or NOD.Idd9.3) carries C57BL/10SnJ (Stock No. 000666) derived genome on Chromosome 4 containing the Idd9.3 region. The Idd9.3 region contains 15 genes, including candidate gene Tnfrsf9 (commonly referred to as 4-1BB or Cd137).

While this strain exhibits significantly diminished diabetes development compared to NOD/MrkTac controls, it is significantly less protected from diabetes development compared to NOD.B10-Idd9.1R1565 (Stock No. 0012309). Tnfrsf9 is biologically more active in this congenic strain than in the control NOD/MrkTac. There is a variation in the Tnfrsf9 coding region between NOD and C57BL/10, including a leucine to proline alteration in the cytoplasmic domain.

This strain may be useful for positional mapping of the Idd9.3 loci and studying candidate genes within the Idd9.3 region.

Development

This NOD congenic strain contains a genomic segment derived from C57BL/10SnJ (Stock No. 000666) introgressed into the Idd9.3 region of Chromosome 4. This congenic region contains 15 genes, including major candidate genes Tnfrsf9 (commonly referred to as 4-1BB or Cd137). This strain was produced by mating mice from NOD/MrkTac to C57BL/10SnJ followed by 13 additional crosses (N14) to NOD/MrkTac with selection for Idd9.3 markers. Siblings carrying the C57BL/10SnJ derived 1.2Mb chromosomal segment denoted by markers AL606914.1 (147Mb) through D4Mit42 (148.2Mb) were intercrossed to make homozygous. 5kSNP Chip Data identifies no C57BL/10SnJ genetic contamination outside of the Chromosome 4 congenic interval. The Type 1 Diabetes Resource received this stock in 2010.

Allele

Symbol: Idd9.3^C57BL/10SnJ
Name: C57BL/10SnJ
MGI accession ID: MGI:3036809
Generation method: QTL
Marker symbol: Idd9.3
Marker name: insulin dependent diabetes susceptibility 9.3
Chromosome: 4
Strain of origin: C57BL/10SnJ
Molecular note: This allele confers resistance to insulin dependent diabetes compared to NOD/MrkTac.