Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.  These mice carry a mutant allele with alanine substitutions for prolines 187 and 190 in exon 4 (CD28 AYAA, or P187,190A), which encodes the SH3 domain-containing protein interaction motif.  Mutant gene product (mRNA) is detected by RT-PCR analysis of thymocytes and peripheral T cells.  Mutant protein expression levels are similar to wildtype protein levels in control mice, as assayed by flow cytometry.  T cells from mice homozygous for the CD28 AYAA KI mutation have impaired IL-2 production and secretion.   Homozygotes fail to develop germinal centers in the spleen, reduced OVA-specific antibody level and display reduced airway hyperresponsiveness after sensitization and challenge with OVA.  Homozygotes exhibit less severe experimental autoimmune encephalomyelitis than wildtype controls, although CNS inflammatory infiltration is similar to wildtype.  This mutant mouse strain may be useful in studies of humoral immune response and T cell proliferation.

This strain carries a mutant allele with alanine substitutions for prolines 187 and 190 in exon 4; homozygotes fail to develop germinal centers in the spleen, have reduced OVA-specific antibody levels, display reduced airway hyperresponsiveness and exhibit less severe experimental autoimmune encephalomyelitis than wildtype controls. This mutant mouse strain may be useful in studies of humoral immune response and T cell proliferation.

This strain is hosted by NIH's MMRRC, which partners with JAX for the distribution of this and other strains. <b>For additional information and to purchase, please visit the MMRRC site.</b>