Enhanced green fluorescent protein (EGFP) is expressed under the control of the mouse Piwil4 (piwi-like homolog 4 (Drosophila); also known as Miwi2) promoter in these transgenic mice. This strain may be useful in studies of male germ cell development and the piRNA (piwi-interacting RNA) pathway.
Gregory Hannon, Cold Spring Harbor Laboratory
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
Enhanced green fluorescent protein (EGFP) is expressed under the control of the mouse Piwil4 (piwi-like homolog 4 (Drosophila); also known as Miwi2) promoter in these transgenic mice. Fluorescence permits detection of expression in vivo, enables biochemical purification of associated protein complexes using anti-GFP antibodies, and facilitates the sorting of expressing germ cells. Transgenic protein, absent in female germ cells, localizes in the nucleus as well as in cytoplasmic granules of male germ cells. Expression patterns are essentially identical in transgenic and wildtype mice, detectable as early as embryonic day 14.5-15.5 (E14.5-15.5) in male germ cells. Expression declines soon after birth, reaching undetectable levels in 4-day-old mice. This strain may be useful in studies of male germ cell development and the piRNA (piwi-interacting RNA) pathway.
An enhanced green fluorescent protein (EGFP) gene was inserted at the N-terminus of mouse Piwil4. The transgenic BAC vector was linearized and introduced to B6SJLF1 embryos. The strain was maintained on a mixed C57BL/6, SJL, and CD1 outbred background by the donating laboratory.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Site of Expression | GFP expression localizes in the nucleus as well as in cytoplasmic granules of male germ cells. |
Allele Name | transgene insertion 1, Gregory J Hannon |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | GFP-MIWI2; Tg(Piwil4-EGFP)1Ghan |
Gene Symbol and Name | Tg(Piwil4/EGFP)1Ghan, transgene insertion 1, Gregory J Hannon |
Gene Synonym(s) | |
Promoter | Piwil4, piwi-like RNA-mediated gene silencing 4, mouse, laboratory |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | GFP expression localizes in the nucleus as well as in cytoplasmic granules of male germ cells. |
Strain of Origin | (C57BL/6 x SJL)F1 |
Chromosome | UN |
Molecular Note | An enhanced green fluorescent protein (EGFP) gene was inserted at the N-terminus of mouse Piwil4 in BAC RP23-414K6. The transgenic BAC vector was linearized and introduced to B6SJLF1 embryos. |
Mutations Made By | Gregory Hannon, Cold Spring Harbor Laboratory |
When maintained as a live colony, hemizygotes or homozygotes may be bred.
When using the STOCK Tg(Piwil4/EGFP)1Ghan/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #012277 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Piwil4/EGFP)1Ghan |
Frozen Mouse Embryo | STOCK Tg(Piwil4/EGFP)1Ghan/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(Piwil4/EGFP)1Ghan/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(Piwil4/EGFP)1Ghan/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Tg(Piwil4/EGFP)1Ghan/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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