Enhanced green fluorescent protein (EGFP) is expressed under the control of the mouse Piwil4 (piwi-like homolog 4 (Drosophila); also known as Miwi2) promoter in these transgenic mice. Fluorescence permits detection of expression in vivo, enables biochemical purification of associated protein complexes using anti-GFP antibodies, and facilitates the sorting of expressing germ cells. Transgenic protein, absent in female germ cells, localizes in the nucleus as well as in cytoplasmic granules of male germ cells. Expression patterns are essentially identical in transgenic and wildtype mice, detectable as early as embryonic day 14.5-15.5 (E14.5-15.5) in male germ cells. Expression declines soon after birth, reaching undetectable levels in 4-day-old mice. This strain may be useful in studies of male germ cell development and the piRNA (piwi-interacting RNA) pathway.

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Enhanced green fluorescent protein (EGFP) is expressed under the control of the mouse Piwil4 (piwi-like homolog 4 (Drosophila); also known as Miwi2) promoter in these transgenic mice. This strain may be useful in studies of male germ cell development and the piRNA (piwi-interacting RNA) pathway.

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STOCK Tg(Piwil4/EGFP)1Ghan/J Frozen Embryo