STOCK Tg(Piwil4/EGFP)1Ghan/J

Stock number: 012277
Research areas: Research Tools

Description

Enhanced green fluorescent protein (EGFP) is expressed under the control of the mouse Piwil4 (piwi-like homolog 4 (Drosophila); also known as Miwi2) promoter in these transgenic mice. This strain may be useful in studies of male germ cell development and the piRNA (piwi-interacting RNA) pathway.

Detailed description

Enhanced green fluorescent protein (EGFP) is expressed under the control of the mouse Piwil4 (piwi-like homolog 4 (Drosophila); also known as Miwi2) promoter in these transgenic mice. Fluorescence permits detection of expression in vivo, enables biochemical purification of associated protein complexes using anti-GFP antibodies, and facilitates the sorting of expressing germ cells. Transgenic protein, absent in female germ cells, localizes in the nucleus as well as in cytoplasmic granules of male germ cells. Expression patterns are essentially identical in transgenic and wildtype mice, detectable as early as embryonic day 14.5-15.5 (E14.5-15.5) in male germ cells. Expression declines soon after birth, reaching undetectable levels in 4-day-old mice. This strain may be useful in studies of male germ cell development and the piRNA (piwi-interacting RNA) pathway.

Development

An enhanced green fluorescent protein (EGFP) gene was inserted at the N-terminus of mouse Piwil4. The transgenic BAC vector was linearized and introduced to B6SJLF1 embryos. The strain was maintained on a mixed C57BL/6, SJL, and CD1 outbred background by the donating laboratory.

Allele

Symbol: Tg(Piwil4/EGFP)1Ghan
Name: transgene insertion 1, Gregory J Hannon
MGI accession ID: MGI:4415287
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(Piwil4/EGFP)1Ghan
Marker name: transgene insertion 1, Gregory J Hannon
Chromosome: UN
Strain of origin: (C57BL/6 x SJL)F1
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: GFP expression localizes in the nucleus as well as in cytoplasmic granules of male germ cells.
Molecular note: An enhanced green fluorescent protein (EGFP) gene was inserted at the N-terminus of mouse Piwil4 in BAC RP23-414K6. The transgenic BAC vector was linearized and introduced to B6SJLF1 embryos.