Mice expressing the IRBP-Tag allele develop hereditary retinoblastomas, pineal tumors, narrowed ocular fissures and exophthalmia. These mice may be useful for studying heritable ocular tumors of photoreceptor cell origin and pineal tumors associated with retinoblastoma.
Daniel M. Albert, University of Wisconsin
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
Mice hemizygous for the IRBP-Tag allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Expression of Tag is regulated by a murine interstitial retinol binding protein (IRBP) promoter (rbp3), which leads to expression of Tag in photoreceptor cells of the developing retina before their terminal differentiation. This expression results in hereditary retinoblastomas and pineal tumors arising from photoreceptor cells and pinealocytes, which may share a common evolutionary ancestor with retinal cells. IRBP-Tag mice develop narrowed ocular fissures by 3 to 4 weeks of age, and exophthalmia by 8 weeks of age. The pineal gland is enlarged by 5 to 6 weeks of age, and is no longer intact by 8 to 10 weeks of age when the tumor covers and invades the cerebral and cerebellar hemispheres. These mice may be useful for studying heritable ocular tumors of photoreceptor cell origin and pineal tumors associated with retinoblastoma.
The IRBP-Tag transgene contains the simian virus 40 (SV40) early region encoding both large T-antigen (Tag) and small t-antigen under the control of murine interstitial retinol binding protein (IRBP) promoter (rbp3). The construct was microinjected into fertilized oocytes obtained from matings of CB6F1/Hsd (BALB/cAnNHsd inbred female bred to C57BL/6NHsd inbred male), and offspring from founder line IT-2 were bred to CB6F1/Hsd nontransgenic mice to establish a colony. Upon arrival at The Jackson Laboratory, mice were bred to CB6F1/J hybrid mice for at least one generation to establish the colony.
Expressed Gene | SV40, E mu enhancer, SV40 |
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Site of Expression |
Allele Name | transgene insertion IT-2, Jolene J Windle |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | IRBP-Tag |
Gene Symbol and Name | Tg(Rbp3-SV40)IT-2Jjw, transgene insertion IT-2, Jolene J Windle |
Gene Synonym(s) | |
Promoter | Rbp3, retinol binding protein 3, interstitial, mouse, laboratory |
Expressed Gene | SV40, E mu enhancer, SV40 |
Strain of Origin | (BALB/cAnNHsd x C57BL/6NHsd)F2 |
Chromosome | UN |
General Note | Transgenic animals develop retinal and pineal tumors by 10-12 weeks of age with 100% penetrance. Transgenic animals carrying the greatest transgene copy number (15-50) also displayed a 25%-30% decrease in body weight compared to non-transgenic animals. |
Molecular Note | 2.7 kb of the SV40 T antigen gene fused to 1.9 kb of mouse RBP3 promoter sequences was used as the transgene. Promoter sequences from -1783 to +101 were used to drive expression of the transgene in photoreceptor cells. Northern blot analysis confirmed transgene expression in the retina and pineal of transgenic animals. |
When maintaining a live colony, hemizygous mice may be bred to CB6F1/J hybrid mice. The donating investigator reports always breeding hemizygous mice to CB6F1 mice.
When using the CB6-Tg(Rbp3-SV40)IT-2Jjw/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #32030 in your Materials and Methods section.
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