This targeted mutation of the neurturin (Nrtn) gene displays reductions in neuron fiber density and neuronal size in the enteric nervous system, reduced gut motility, reduced parasympathetic and sensory innervation and may be useful in studies of the enteric, parasympathetic and sensory nervous systems
Jeffrey Milbrandt, Washington University School of Medicine, St. Louis
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Nrtn | neurturin |
Mice that are homozygous for the targeted mutation are viable, fertile and normal in size. Homozygotes exhibit a decrease in small fiber density in the small intestine, colon and duodenum, reduced myenteric ganglion cell size, and reduced gut motility. By two months of age, mice can be identified by eyelid droopiness, crusting around the eye, eyelid thickening and reduced tear production most likely the result of reduced parasympathetic innervation of the lacrimal gland. Parasympathetic neurons in the submandibular salivary gland and the ciliary ganglion are reduced in number, despite apparently normal function. Otic ganglion cells are reduced in size, but not in number. This mutant mouse strain may be useful in studies of the enteric, parasympathetic and sensory nervous systems.
A targeting vector containing the neomycin resistance gene was used to disrupt a 1.5 kb region including entire coding region of the protein and extending into the 3' untranslated region. The construct was electroporated into 129X1/SvJ-derived RW4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to C57BL/6J and maintained by intercross. Upon arrival, mice were bred to C57BL/6 for at least 1 generation to establish the colony.
Allele Name | targeted mutation 1, Jeffrey Milbrandt |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Nrtn-; NTN- |
Gene Symbol and Name | Nrtn, neurturin |
Gene Synonym(s) | |
Strain of Origin | 129X1/SvJ |
Chromosome | 17 |
Molecular Note | The gene was disrupted by replacement of the entire coding region with a neomycin resistance cassette via homologous recombination. Absence of gene expression in homozygous mutant animals was confirmed by RT-PCR analysis of heart mRNA. |
Mutations Made By | Jeffrey Milbrandt, Washington University School of Medicine, St. Louis |
While maintaining a live colony, these mice are bred as homozygotes.
When using the B6;129X1-Nrtntm1Jmi/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #012238 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous for Nrtn<tm1Jmi> |
Frozen Mouse Embryo | B6;129X1-Nrtn<tm1Jmi>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129X1-Nrtn<tm1Jmi>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129X1-Nrtn<tm1Jmi>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129X1-Nrtn<tm1Jmi>/J Frozen Embryo | $3373.50 |
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