These mice possess loxP sites on either side of the second exon (first coding exon) in the Osmr (oncostatin M receptor) gene. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene. This strain may be useful in further characterization of this multifunctional cytokine.
Jeremy Nathans, Johns Hopkins University
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Osmr | oncostatin M receptor |
These mice possess loxP sites on either side of the second exon (first coding exon) in the Osmr (oncostatin M receptor) gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene. Expression in the retina and retinal pigment epithelium (RPE) is undetectable in protein blots after crosses with a germline cre strain. This strain may be useful in further characterization of this multifunctional cytokine.
The second exon (first coding exon) of the targeted gene was flanked by two LoxP sites. Adjacent to the second LoxP site is an Frt site left over after the excision of the PGK-neomycin selectable marker. This mutation was created in (129X1/SvJ x 129S1/Sv)F1- Kitl+-derived R1 embryonic stem (ES) cells. The strain was maintained on a mixed C57BL/6 and 129 genetic background by the donating laboratory.
Allele Name | targeted mutation 1.1, Jeremy Nathans |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | |
Gene Symbol and Name | Osmr, oncostatin M receptor |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 15 |
Molecular Note | The second exon (first coding exon) of the targeted gene was flanked by two LoxP sites. Adjacent to the second LoxP site is an Frt site left over after the excision of the PGK-neomycin selectable marker. |
When maintained as a live colony, homozygous mice may be bred together.
When using the B6;129-Osmrtm1.1Nat/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #011081 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype Osmr<tm1.1Nat> |
Frozen Mouse Embryo | B6;129-Osmr<tm1.1Nat>/J | $2595.00 |
Frozen Mouse Embryo | B6;129-Osmr<tm1.1Nat>/J | $2595.00 |
Frozen Mouse Embryo | B6;129-Osmr<tm1.1Nat>/J | $3373.50 |
Frozen Mouse Embryo | B6;129-Osmr<tm1.1Nat>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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