This Hoxa11eGFP knock-in strain expresses Green Fluorescent Protein from the endogenous Hoxa11 (homeo box A11) locus. This mutant mouse strain may be useful in studies of embryonic development and organogenesis.
Deneen Wellik, University of Michigan
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter, Null/Knockout) | Hoxa11 | homeobox A11 |
This strain expresses Green Fluorescent Protein from the targeted locus. Localization of Green Fluorescent Protein closely mimics the endogenous gene (mRNA) expression pattern. Fluorescence is first detected at embryonic day 9.5 in the tail tip and forelimb bud. Fluorescence is later detected in the tail, neural tube and limb buds.
Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygotes are infertile.
A targeting vector containing Green Fluorescent Protein cDNA, an SV40 T-antigen stop sequence and a self-excising ACN (neomycin resistant) selection cassette was used to disrupt exon 1. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+ derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric male animals were crossed to C57BL/6 mice, and then backcrossed to C57BL/6 for at least 5 generations. SNP (single nucleotide polymorphism) analysis performed by The Jackson Laboratory revealed that the Donating Investigator used C57BL/6N. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J at least once to establish the colony.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Site of Expression | GFP is first detected at embryonic day 9.5 in the tail tip and forelimb bud. Fluorescence is later detected in the tail, neural tube and limb buds. |
Allele Name | targeted mutation 1, Deneen M Wellik |
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Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | Hoxa11eGFP |
Gene Symbol and Name | Hoxa11, homeobox A11 |
Gene Synonym(s) | |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | GFP is first detected at embryonic day 9.5 in the tail tip and forelimb bud. Fluorescence is later detected in the tail, neural tube and limb buds. |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 6 |
Molecular Note | An eGFP construct was inserted 18 bp downstream of the start site in exon 1 replacing most of exon 1. This was followed by an SV40 T-antigen stop sequence and a self-excising neo cassette in reverse orientation. Cre mediated excision of the neo cassette occurs in the testes. |
Mutations Made By | Deneen Wellik, University of Michigan |
When maintaining a live colony, these mice can be bred as heterozygotes. Homozygotes are viable but infertile.
When using the B6.129-Hoxa11tm1Dmwe/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #011036 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Hoxa11<tm1Dmwe> |
Frozen Mouse Embryo | B6.129-Hoxa11<tm1Dmwe>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129-Hoxa11<tm1Dmwe>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129-Hoxa11<tm1Dmwe>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129-Hoxa11<tm1Dmwe>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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