B6.129-Hoxa11^tm1Dmwe/J

Stock number: 011036
Research areas: Neurobiology Research, Research Tools

Description

This Hoxa11eGFP knock-in strain expresses Green Fluorescent Protein from the endogenous Hoxa11 (homeo box A11) locus. This mutant mouse strain may be useful in studies of embryonic development and organogenesis.

Detailed description

This strain expresses Green Fluorescent Protein from the targeted locus. Localization of Green Fluorescent Protein closely mimics the endogenous gene (mRNA) expression pattern. Fluorescence is first detected at embryonic day 9.5 in the tail tip and forelimb bud. Fluorescence is later detected in the tail, neural tube and limb buds. Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygotes are infertile.

Development

A targeting vector containing Green Fluorescent Protein cDNA, an SV40 T-antigen stop sequence and a self-excising ACN (neomycin resistant) selection cassette was used to disrupt exon 1. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl⁺ derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric male animals were crossed to C57BL/6 mice, and then backcrossed to C57BL/6 for at least 5 generations. SNP (single nucleotide polymorphism) analysis performed by The Jackson Laboratory revealed that the Donating Investigator used C57BL/6N. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J at least once to establish the colony.

Allele

Symbol: Hoxa11^tm1Dmwe
Name: targeted mutation 1, Deneen M Wellik
MGI accession ID: MGI:3814861
Generation method: Targeted
Attributes: Reporter; Null/Knockout
Marker symbol: Hoxa11
Marker name: homeobox A11
Chromosome: 6
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: GFP is first detected at embryonic day 9.5 in the tail tip and forelimb bud. Fluorescence is later detected in the tail, neural tube and limb buds.
Molecular note: An eGFP construct was inserted 18 bp downstream of the start site in exon 1 replacing most of exon 1. This was followed by an SV40 T-antigen stop sequence and a self-excising neo cassette in reverse orientation. Cre mediated excision of the neo cassette occurs in the testes.