A loxP-flanked neomycin resistance cassette was introduced to intron 3 of the targeted gene in TC1 129S6/SvEvTac-derived embryonic stem (ES) cells. The floxed neomycin cassette was excised with germline protamine (Prm) PC3-cre recombinase on a C57BL/6 background, leaving a single loxP site. This strain was backcrossed to C57BL/6 for ten generations by the donating laboratory.
|Allele Name||targeted mutation 4.1, Roger J Davis|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Bmf, BCL2 modifying factor|
|Strain of Origin||129S6/SvEvTac|
|Molecular Note||A floxed neo cassette was inserted within intron 3. The neo cassette was then excised with Cre mediated recombination leaving a single loxP site in intron 3.|
|Mutations Made By|| |
Roger Davis, University of Massachusetts Medical Sch.
When maintained as a live colony, heterozygotes or homozygotes may be bred.
When using the B6.129S6-Bmftm4.1Rjd/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #011024 in your Materials and Methods section.