B6.129S6-Bmf^tm4.1Rjd/J

Stock number: 011024
Research areas: Apoptosis Research

Description

This targeted mutation strain may be used as a control for Bmf S74A (see Stock No. 011022) and S74D (see Stock No. 011023) mutant mice in studies of JNK phosphorylation-induced apoptosis.

Detailed description

This targeted mutation strain may be used as a control for Bmf S74A (see Stock No. 011022) and S74D (see Stock No. 011023) mutant mice in studies of JNK phosphorylation-induced apoptosis.

Development

A loxP-flanked neomycin resistance cassette was introduced to intron 3 of the targeted gene in TC1 129S6/SvEvTac-derived embryonic stem (ES) cells. The floxed neomycin cassette was excised with germline protamine (Prm) PC3-cre recombinase on a C57BL/6 background, leaving a single loxP site. This strain was backcrossed to C57BL/6 for ten generations by the donating laboratory.

Allele

Symbol: Bmf^tm4.1Rjd
Name: targeted mutation 4.1, Roger J Davis
MGI accession ID: MGI:5287003
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Bmf
Marker name: BCL2 modifying factor
Chromosome: 2
Strain of origin: 129S6/SvEvTac
Molecular note: A floxed neo cassette was inserted within intron 3. The neo cassette was then excised with Cre mediated recombination leaving a single loxP site in intron 3.