Homozygous Bmf (BCL2 modifying factor) targeted mutant mice lack expression of its protein which bears only the pro-apoptotic BH3 domain.This strain may be useful in studies of apoptosis or female reproductive tract defects.
Roger J Davis, University of Massachusetts Medical Sch.
Homozygous Bmf (BCL2 modifying factor) targeted mutant mice lack expression of its protein which bears only the pro-apoptotic BH3 domain. Expression in brain, spleen, thymus and lymph node tissues is eliminated. Female homozygotes exhibit an imperforate vagina and hydrometrocolpos (22% penetrance). Male and female mice exhibit increased numbers of B cells.
Exons 3 and 4 were replaced by a neomycin resistance cassette in 129S6/SvEvTac-derived TC1 embryonic stem (ES) cells. The strain was backcrossed to C57BL/6 for ten generations by the donating laboratory.
|Allele Name||targeted mutation 1, Roger J Davis|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Bmf, BCL2 modifying factor|
|Strain of Origin||129S6/SvEvTac|
|Molecular Note||Exons 3 and 4 were replaced with a neo cassette by homologous recombination.|
|Mutations Made By|| |
Roger Davis, University of Massachusetts Medical Sch.
When maintained as a live colony, heterozygous crosses are generally used, but some homozygotes may also be bred. The donating laboratory successfully maintains their colony using homozygote x homozygote crosses. Female homozygotes may exhibit an imperforate vagina and hydrometrocolpos (22% penetrance).
When using the B6.129S6-Bmftm1Rjd/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #011021 in your Materials and Methods section.