B6.129S6-Bmf^tm1Rjd/J

Stock number: 011021
Research areas: Apoptosis Research, Reproductive Biology Research

Description

Homozygous Bmf (BCL2 modifying factor) targeted mutant mice lack expression of its protein which bears only the pro-apoptotic BH3 domain.This strain may be useful in studies of apoptosis or female reproductive tract defects.

Detailed description

Homozygous Bmf (BCL2 modifying factor) targeted mutant mice lack expression of its protein which bears only the pro-apoptotic BH3 domain. Expression in brain, spleen, thymus and lymph node tissues is eliminated. Female homozygotes exhibit an imperforate vagina and hydrometrocolpos (22% penetrance). Male and female mice exhibit increased numbers of B cells.

Development

Exons 3 and 4 were replaced by a neomycin resistance cassette in 129S6/SvEvTac-derived TC1 embryonic stem (ES) cells. The strain was backcrossed to C57BL/6 for ten generations by the donating laboratory.

Allele

Symbol: Bmf^tm1Rjd
Name: targeted mutation 1, Roger J Davis
MGI accession ID: MGI:4421178
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Bmf
Marker name: BCL2 modifying factor
Chromosome: 2
Strain of origin: 129S6/SvEvTac
Molecular note: Exons 3 and 4 were replaced with a neo cassette by homologous recombination.