Homozygous Bmf (BCL2 modifying factor) targeted mutant mice lack expression of its protein which bears only the pro-apoptotic BH3 domain.This strain may be useful in studies of apoptosis or female reproductive tract defects.
Roger J Davis, University of Massachusetts Medical Sch.
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Bmf | BCL2 modifying factor |
Homozygous Bmf (BCL2 modifying factor) targeted mutant mice lack expression of its protein which bears only the pro-apoptotic BH3 domain. Expression in brain, spleen, thymus and lymph node tissues is eliminated. Female homozygotes exhibit an imperforate vagina and hydrometrocolpos (22% penetrance). Male and female mice exhibit increased numbers of B cells.
Exons 3 and 4 were replaced by a neomycin resistance cassette in 129S6/SvEvTac-derived TC1 embryonic stem (ES) cells. The strain was backcrossed to C57BL/6 for ten generations by the donating laboratory.
Allele Name | targeted mutation 1, Roger J Davis |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Bmf- |
Gene Symbol and Name | Bmf, BCL2 modifying factor |
Gene Synonym(s) | |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 2 |
Molecular Note | Exons 3 and 4 were replaced with a neo cassette by homologous recombination. |
Mutations Made By | Roger Davis, University of Massachusetts Medical Sch. |
When maintained as a live colony, heterozygous crosses are generally used, but some homozygotes may also be bred. The donating laboratory successfully maintains their colony using homozygote x homozygote crosses. Female homozygotes may exhibit an imperforate vagina and hydrometrocolpos (22% penetrance).
When using the B6.129S6-Bmftm1Rjd/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #011021 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Bmf<tm1Rjd> |
Frozen Mouse Embryo | B6.129S6-Bmf<tm1Rjd>/J | $2595.00 |
Frozen Mouse Embryo | B6.129S6-Bmf<tm1Rjd>/J | $2595.00 |
Frozen Mouse Embryo | B6.129S6-Bmf<tm1Rjd>/J | $3373.50 |
Frozen Mouse Embryo | B6.129S6-Bmf<tm1Rjd>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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