These mice carry a beta-geo reporter integrated in the Map4k4 gene and may be useful in studies of Mapk8 (JNK) activation.
Roger J Davis, University of Massachusetts Medical Sch.
These mice, developed using gene trap technology, carry a beta-geo reporter integrated in the Map4k4 (mitogen-activated protein kinase kinase kinase kinase 4) gene. The mutant allele is expressed in the liver. Heterozygous mice show no overt phenotype. Homozygotes are early embryonic lethal. This strain may be useful in studies of Mapk8 (JNK) activation.
A gene trap vector (pGT0Lxf) containing the reporter gene beta-geo inserted between exons 2 and 3 was electroporated into 129P2/OlaHsd derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice for ten generations by the donating laboratory.
|Allele Name||gene trap RRS090, BayGenomics|
|Allele Type||Gene trapped|
|Gene Symbol and Name||Map4k4, mitogen-activated protein kinase kinase kinase kinase 4|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||Trapped gene association determined by genome coordinate overlap and strand.|
|Mutations Made By|| |
Roger Davis, University of Massachusetts Medical Sch.
When maintained as a live colony, heterozygotes may be bred. Homozygotes are early embryonic lethal.
When using the B6.129P2-Map4k4Gt(RRS090)Byg/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #011020 in your Materials and Methods section.