B6.129P2-Map4k4^{Gt(RRS090)Byg}/J

Stock number: 011020
Research areas: Cell Biology Research

Description

These mice carry a beta-geo reporter integrated in the Map4k4 gene and may be useful in studies of Mapk8 (JNK) activation.

Detailed description

These mice, developed using gene trap technology, carry a beta-geo reporter integrated in the Map4k4 (mitogen-activated protein kinase kinase kinase kinase 4) gene. The mutant allele is expressed in the liver. Heterozygous mice show no overt phenotype. Homozygotes are early embryonic lethal. This strain may be useful in studies of Mapk8 (JNK) activation.

Development

A gene trap vector (pGT0Lxf) containing the reporter gene beta-geo inserted between exons 2 and 3 was electroporated into 129P2/OlaHsd derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice for ten generations by the donating laboratory.

Allele

Symbol: Map4k4^{Gt(RRS090)Byg}
Name: gene trap RRS090, BayGenomics
MGI accession ID: MGI:4330340
Generation method: Gene trapped
Marker symbol: Map4k4
Marker name: mitogen-activated protein kinase kinase kinase kinase 4
Marker synonyms: 9430080K19Rik; FLH21957; HEL-S-31; HGK; MEKKK4; Nik
Chromosome: 1
Strain of origin: 129P2/OlaHsd
Molecular note: Trapped gene association determined by genome coordinate overlap and strand.