B6.129S6-Mapk8ip1^tm2.1Rjd/J

Stock number: 011019
Research areas: Diabetes and Obesity Research

Description

Serine 59 of the Mapk8ip1 (mitogen-activated protein kinase 8 interacting protein 1) gene is replaced with an asparagine, to correspond with a human polymorphism associated with type 2 diabetes. Feeding homozygous mice a high-fat diet causes a reproducible, but modest, reduction in JNK (Mapk8, mitogen-activated protein kinase 8) activation, but does not increase the susceptibility of mice to diet-induced obesity or insulin resistance.

Detailed description

Serine 59 of the Mapk8ip1 (mitogen-activated protein kinase 8 interacting protein 1) gene is replaced with an asparagine, to correspond with a human polymorphism associated with type 2 diabetes. Feeding homozygous mice a high-fat diet causes a reproducible, but modest, reduction in JNK (Mapk8, mitogen-activated protein kinase 8) activation, but does not increase the susceptibility of mice to diet-induced obesity or insulin resistance.

Development

A loxP-flanked neomycin resistance cassette was inserted into intron 1, and a Ser59Asn (ATCTGC to ATTAAT) mutation was created in exon 2, introducing an Ase1 restriction site. TC1 embryonic stem (ES) cells derived from 129S6/SvEvTac were used to create the mutation. The floxed neomycin cassette was excised through a germline C57BL/6 background protamine (Prm) PC3-cre recombinase cross. This strain was backcrossed to C57BL/6 for 10 generations by the donating laboratory.

Allele

Symbol: Mapk8ip1^tm2.1Rjd
Name: targeted mutation 2.1, Roger J Davis
MGI accession ID: MGI:4360022
Generation method: Targeted
Attributes: Not Applicable
Marker symbol: Mapk8ip1
Marker name: mitogen-activated protein kinase 8 interacting protein 1
Chromosome: 2
Strain of origin: 129S6/SvEvTac
Molecular note: Exon 2 contained a point mutation that resulted in the amino acid substitution of asparagine for serine at position 59 (S59N). A floxed neo cassette was inserted downsteam of the modified exon 2 was removed by cre mediated recombination.