These P25 TCR-Tg transgenic mice contain CD4 + T cells expressing a T-cell antigen receptor that recognizes Mycobacterium tuberculosis antigen. These mice may be useful for studying the process of the adaptive immune response to M. tuberculosis infection in the lungs.
Joel D Ernst, New York University School of Medicine
Genetic Background | Generation |
---|---|
|
Allele Type |
---|
Transgenic (Inserted expressed sequence) |
These P25 TCR-Tg mice contain CD4 + T cells expressing a transgenic T-cell antigen receptor that recognizes peptide 25 (aa 240-254) of Mycobacterium tuberculosis Antigen 85B bound to I-Ab. Homozygotes are viable, fertile, and normal in size. These transgenic mice, with T cell response specific to M. tuberculosis infection, may be useful for studying the process of the adaptive immune response to M. tuberculosis infection in the lungs. These mice exhibit an immune response that is not dependant on bacteria number in the tissue, but rather on movement of bacterium into the draining nodes of the lungs.
The P25TCR transgenic mice contain a T-cell antigen receptor (TCR) that recognizes peptide 25 (aa 240-254) of Mycobacterium tuberculosis Antigen 85B bound to I-Ab under the control of the H-2Kb promoter. Specifically, two transgenes were created, each containing either cDNA encoding the Vα5 or the Vβ11 gene, and a polyadenylation (polyA) sequence. The constructs were coinjected into fertilized C57BL/6 oocytes and the resulting offspring were bred to C57BL/6 mice to establish a colony. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
Expressed Gene | Tcrb, T cell receptor beta chain, mouse, laboratory |
---|---|
Expressed Gene | Tcra, T cell receptor alpha chain, mouse, laboratory |
Site of Expression |
Allele Name | transgene insertion P25, Kiyoshi Takatsu |
---|---|
Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | P25 TCR-Tg |
Gene Symbol and Name | Tg(H2-Kb-Tcra,-Tcrb)P25Ktk, transgene insertion P25, Kiyoshi Takatsu |
Gene Synonym(s) | |
Promoter | H2-K, histocompatibility 2, K region, mouse, laboratory |
Expressed Gene | Tcrb, T cell receptor beta chain, mouse, laboratory |
Expressed Gene | Tcra, T cell receptor alpha chain, mouse, laboratory |
Strain of Origin | C57BL/6 |
Chromosome | UN |
Molecular Note | The P25TCR transgenic mice contain a T-cell antigen receptor (TCR) that recognizes peptide 25 (aa 240-254) of Mycobacterium tuberculosis Antigen 85B bound to I-Ab under the control of the H-2Kb promoter. Specifically, two transgenes were created, each containing either cDNA encoding the Valpha5 or the Vbeta11 gene, and a polyadenylation (polyA) sequence. Line P25 was created. |
When maintaining a live colony, transgene carrier mice may be bred with wildtype (noncarrier) mice from the colony or with C57BL/6J inbred mice (Stock No. 000664). The Donating Investigator maintains these mice as homozygotes.
When using the P25 TCR-Tg mouse strain in a publication, please cite the originating article(s) and include JAX stock #011005 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous or Non carrier for Tg(H2-K<b>-Tcra,-Tcrb)P25Ktk |
Frozen Mouse Embryo | C57BL/6-Tg(H2-K<b>-Tcra -Tcrb)P25Ktk/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Tg(H2-K<b>-Tcra -Tcrb)P25Ktk/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Tg(H2-K<b>-Tcra -Tcrb)P25Ktk/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | C57BL/6-Tg(H2-K<b>-Tcra -Tcrb)P25Ktk/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.