C57BL/6-Tg(H2-K^b-Tcra,-Tcrb)P25Ktk/J

Stock number: 011005
Product name: P25 TCR-Tg
Research areas: Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

These P25 TCR-Tg transgenic mice contain CD4 + T cells expressing a T-cell antigen receptor that recognizes Mycobacterium tuberculosis antigen. These mice may be useful for studying the process of the adaptive immune response to M. tuberculosis infection in the lungs.

Detailed description

These P25 TCR-Tg mice contain CD4 + T cells expressing a transgenic T-cell antigen receptor that recognizes peptide 25 (aa 240-254) of Mycobacterium tuberculosis Antigen 85B bound to I-Ab. Homozygotes are viable, fertile, and normal in size. These transgenic mice, with T cell response specific to M. tuberculosis infection, may be useful for studying the process of the adaptive immune response to M. tuberculosis infection in the lungs. These mice exhibit an immune response that is not dependant on bacteria number in the tissue, but rather on movement of bacterium into the draining nodes of the lungs.

Development

The P25TCR transgenic mice contain a T-cell antigen receptor (TCR) that recognizes peptide 25 (aa 240-254) of Mycobacterium tuberculosis Antigen 85B bound to I-A^b under the control of the H-2K^b promoter. Specifically, two transgenes were created, each containing either cDNA encoding the Vα5 or the Vβ11 gene, and a polyadenylation (polyA) sequence. The constructs were coinjected into fertilized C57BL/6 oocytes and the resulting offspring were bred to C57BL/6 mice to establish a colony. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Tg(H2-K^b-Tcra,-Tcrb)P25Ktk
Name: transgene insertion P25, Kiyoshi Takatsu
MGI accession ID: MGI:4454446
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(H2-K^b-Tcra,-Tcrb)P25Ktk
Marker name: transgene insertion P25, Kiyoshi Takatsu
Chromosome: UN
Strain of origin: C57BL/6
Expressed genes: Tcra,T cell receptor alpha chain,mouse, laboratory; Tcrb,T cell receptor beta chain,mouse, laboratory
Molecular note: The P25TCR transgenic mice contain a T-cell antigen receptor (TCR) that recognizes peptide 25 (aa 240-254) of Mycobacterium tuberculosis Antigen 85B bound to I-Ab under the control of the H-2Kb promoter. Specifically, two transgenes were created, each containing either cDNA encoding the Valpha5 or the Vbeta11 gene, and a polyadenylation (polyA) sequence. Line P25 was created.