Overview

Also Known As: malformed digits 2 Jackson

These mutant mice are characterized by missing toes and a coarse tail.

Genetic overview

Genetic Background	Generation

Lyst^bg

Allele Type	Gene Symbol	Gene Name
Radiation induced	Lyst	lysosomal trafficking regulator

Foxq1^sa

Allele Type	Gene Symbol	Gene Name
Radiation induced	Foxq1	forkhead box Q1

Allele Type	Gene Symbol	Gene Name
Spontaneous	Lrp4	low density lipoprotein receptor-related protein 4

Research Applications

Research Tools
Immunology, Inflammation and Autoimmunity Research
Hematological Research
Internal/Organ Research
Mouse/Human Gene Homologs
Developmental Biology Research
Metabolism Research
Cardiovascular Research

View All Research Applications

Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Important Note

The satin (Foxq1^sa), beige (Lyst^bg), and retinal degeneration (Pde6b^rd1) mutations are still segregating in this mixed genetic background

Detailed Description

Mice homozygous for the Lrp4^mdig-2J mutation have toes missing from each foot, have a coarse tail, and some, but not all, have one or two extra upper incisors.

Development

The mutation malformed digits 2 Jackson (mdig-2J) arose spontaneously at The Jackson Laboratory in 2008 on the SB/LeJ inbred background, which is homozygous for the satin and beige mutations. This mutant subline was outcrossed once to C3H/HeSnJ and the resulting progeny were intercrossed. This line has been maintained since then by sibling intercrossing heterozygotes and homozygotes and the Lyst^bg and Foxq1^sa mutations have not been bred out of the line.

Control Suggestions

Heterozygote from the colony

Additional Information

Considerations for Choosing Controls

Selected References

Curtain MM; Donahue LR. 2010. A Mouse Mutation in Lrp4 MGI Direct Data SubmissionMGI: J:165544

View All References

Genetics

Lyst^bg

Allele Symbol: Lyst^bg

Allele Name	beige
Allele Type	Radiation induced
Allele Synonym(s)	beige; Lyst^bg
Gene Symbol and Name	Lyst, lysosomal trafficking regulator
Gene Synonym(s)	beige; CHS; D13Sfk13; CHS1; DNA segment, Chr 13, Stephen F. Kingsmore 13; D13Sfk13; bg; Beige
Strain of Origin	C3H/Rl
Chromosome	13
Molecular Note	This allele is an insertion of a partial LINE 1 repetitive element into an intron of the gene. The insertion included only the most 3' 1097 bp of the element producing a frameshift mutation resulting in a truncated protein predicted to be missing the last 1442 amino acids.

Foxq1^sa

Allele Symbol: Foxq1^sa

Allele Name	satin
Allele Type	Radiation induced
Allele Synonym(s)	Foxq1^sa; satin
Gene Symbol and Name	Foxq1, forkhead box Q1
Gene Synonym(s)	Hfh1; Hfh1l; HFH1; Hfh1l; sa; HNF-3/forkhead homolog 1 like; HNF-3/forkhead homolog 1; HFH-1; sa; satin; Hfh1; Hfh-1
Strain of Origin	(101/Rl x C3H/Rl)F1
Chromosome	13
Molecular Note	This allele occurred in descendants of a gamma irradiation experiment. The underlying mutation was identified as a 67 bp intragenic deletion. The deletion causes a frame shift resulting in a truncated 376 amino acid protein but does not affect the first 228 residues, including the characteristic WH DNA-binding domain. The mutant protein is predicted to bind to its DNA targets but fails at transcriptional activation or repression because the specific C-terminal domains are missing.

Lrp4^mdig-2J

Allele Symbol: Lrp4^mdig-2J

Allele Name	malformed digits 2 Jackson
Allele Type	Spontaneous
Allele Synonym(s)	malformed digits 2 Jackson; Lrp4^mdig-2J
Gene Symbol and Name	Lrp4, low density lipoprotein receptor-related protein 4
Gene Synonym(s)	CLSS; RIKEN cDNA 6430526J12 gene; 6430526J12Rik; mdig; LRP-4; LRP10; malformed digits; SOST2; 6430526J12Rik; mdig; MEGF7; CMS17; Megf7
Strain of Origin	SB/LeJ
Chromosome	2
Molecular Note	Proven allelic with Lrp4^mdig by failed complementation test.

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Genotype: Lyst^bg related

Research Tools
- Immunology, Inflammation and Autoimmunity Research
  - NK Cell Deficiency
Immunology, Inflammation and Autoimmunity Research
- CD Antigens, Antigen Receptors, and Histocompatibility Markers
- Immunodeficiency
- Intracellular Signaling Molecules
Hematological Research
- Platelet Defects
  - platelet storage pool deficiency
- Immunological Defects
Internal/Organ Research
- Kidney Defects
  - lysosomal enzyme abnormalities
Mouse/Human Gene Homologs
- Chediak-Higashi syndrome
Metabolism Research
Cardiovascular Research
- Atherosclerosis
Dermatology Research
- Color and White Spotting Defects

Genotype: Lrp4^mdig-2J related

Developmental Biology Research
- Craniofacial and Palate Defects
- Skeletal Defects
  - Oligodactyly

Genotype: Foxq1^sa related

Dermatology Research
- Skin and Hair Texture Defects

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Lrp4^mdig-2J/Lrp4^mdig-2J
involves: C3H/HeSnJ * SB/LeJ

craniofacial phenotype

supernumerary incisors
- some homozygotes, but not all, have one or two extra upper incisors

growth/size/body region phenotype

supernumerary incisors
- some homozygotes, but not all, have one or two extra upper incisors

limbs/digits/tail phenotype

abnormal tail morphology
- coarse tail

oligodactyly
- all 4 feet have toes missing

skeleton phenotype

supernumerary incisors
- some homozygotes, but not all, have one or two extra upper incisors

References

Curtain MM; Donahue LR. 2010. A Mouse Mutation in Lrp4 MGI Direct Data SubmissionMGI: J:165544

Additional - Foxq1^sa related

Additional - Lrp4^mdig-2J related

Additional - Lyst^bg related

Technical Support

Contact Technical Support

Genotyping Protocols
- Genotyping resources and troubleshooting
Citation
When using the malformed digits 2 Jackson mouse strain in a publication, please cite the originating article(s) and include JAX stock #010968 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous for Lrp4<mdig-2J>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

Terms Of Use

Terms of Use

General Terms and Conditions

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCT(S)" means biological materials supplied by JACKSON, and their derivatives. "SERVICES" means projects conducted by JACKSON for other parties that may include but are not limited to the use of MICE or PRODUCTS. "RECIPIENT" means each recipient of MICE, PRODUCTS, or SERVICES provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE, PRODUCTS or SERVICES from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON’s prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED "AS IS". JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

Credit for PRODUCTS or SERVICES

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of, PRODUCTS or SERVICES, JACKSON will, at its option, provide credit or replacement for the PRODUCT received or the SERVICES provided; JACKSON makes no other representations and this shall be the exclusive remedy of the purchaser. Please note specific policy for live mice.

Animal Care and Use for SERVICES

Consistent with the requirement for a written understanding regarding animal care and use, the JACKSON Animal Care and Use Committee will review the animal care and use protocol(s) associated with any SERVICES to be performed at JACKSON, and JACKSON shall have ultimate responsibility and authority for the care of animals while on site or in JACKSON custody.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE, PRODUCTS or SERVICES are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or SERVICES shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or SERVICES by JACKSON.

Also Known As: malformed digits 2 Jackson

Genetic overview

Research Applications

Base Price

Important Note

Detailed Description

Development

Control Suggestions

Additional Information

Selected References

Lystbg

Allele Symbol: Lystbg

Foxq1sa

Allele Symbol: Foxq1sa

Lrp4mdig-2J

Allele Symbol: Lrp4mdig-2J

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Genotype: Lystbg related

Genotype: Lrp4mdig-2J related

Genotype: Foxq1sa related

Mammalian Phenotype Terms by Genotype

Genotype: Lrp4mdig-2J/Lrp4mdig-2Jinvolves: C3H/HeSnJ * SB/LeJ

craniofacial phenotype

growth/size/body region phenotype

limbs/digits/tail phenotype

skeleton phenotype

References

Additional - Foxq1sa related

Additional - Lrp4mdig-2J related

Additional - Lystbg related

Genotyping Protocols

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Lyst^bg

Allele Symbol: Lyst^bg

Foxq1^sa

Allele Symbol: Foxq1^sa

Lrp4^mdig-2J

Allele Symbol: Lrp4^mdig-2J

Genotype: Lyst^bg related

Genotype: Lrp4^mdig-2J related

Genotype: Foxq1^sa related

Genotype: Lrp4^mdig-2J/Lrp4^mdig-2J
involves: C3H/HeSnJ * SB/LeJ

Additional - Foxq1^sa related

Additional - Lrp4^mdig-2J related

Additional - Lyst^bg related