B6.129S2-Pms2^tm1Lisk/J

Stock number: 010945
Product name: Pms2-
Research areas: Cancer Research, Reproductive Biology Research

Description

This targeted mutation of the postmeiotic segregation increased 2 gene (Pms2) exhibits male infertility, microsatellite DNA instability and increased tumor incidence. This strain may useful in the studies of spermatogenesis, cancer and DNA mismatch repair.

Detailed description

Mice that are homozygous for the targeted mutation are viable, but show an increased incidence of sarcomas and lymphomas. Sperm, tail and tumor cells from homozygous mice exhibit microsatellite instability. Male mice on the mixed C57BL/6 and 129S2 background are infertile with reduced numbers of spermatozoa. Spermatozoa exhibit misshapen heads, truncated, irregular flagella. This strain may be useful in the studies of spermatogenesis, cancer and DNA mismatch repair.

Development

A targeting vector was designed to replace exon 2 of the targeted gene with a neomycin resistance cassette. The construct was electroporated into 129S2/SvPas-derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts, and chimeric males were bred to C57BL/6 females. Heterozygous offspring were bred to C57BL/6 mice for greater than 10 generations prior to arrival at The Jackson Laboratory.

Allele

Symbol: Pms2^tm1Lisk
Name: targeted mutation 1, Michael Liskay
MGI accession ID: MGI:1857947
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Pms2
Marker name: PMS1 homolog2, mismatch repair system component
Chromosome: 5
Strain of origin: 129S2/SvPas
Molecular note: A neomycin cassette was inserted into exon 2, replacing a highly conserved region of the gene. Protein was not detectable by Western analysis in fibroblast cultures from homozygous mutant animals.