This targeted mutation of the postmeiotic segregation increased 2 gene (Pms2) exhibits male infertility, microsatellite DNA instability and increased tumor incidence. This strain may useful in the studies of spermatogenesis, cancer and DNA mismatch repair.
Dr. R. M. Liskay, Oregon Health Sciences University
Mice that are homozygous for the targeted mutation are viable, but show an increased incidence of sarcomas and lymphomas. Sperm, tail and tumor cells from homozygous mice exhibit microsatellite instability. Male mice on the mixed C57BL/6 and 129S2 background are infertile with reduced numbers of spermatozoa. Spermatozoa exhibit misshapen heads, truncated, irregular flagella. This strain may be useful in the studies of spermatogenesis, cancer and DNA mismatch repair.
A targeting vector was designed to replace exon 2 of the targeted gene with a neomycin resistance cassette. The construct was electroporated into 129S2/SvPas-derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts, and chimeric males were bred to C57BL/6 females. Heterozygous offspring were bred to C57BL/6 mice for greater than 10 generations prior to arrival at The Jackson Laboratory.
|Allele Name||targeted mutation 1, Michael Liskay|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Pms2, PMS1 homolog2, mismatch repair system component|
|Strain of Origin||129S2/SvPas|
|Molecular Note||A neomycin cassette was inserted into exon 2, replacing a highly conserved region of the gene. Protein was not detectable by Western analysis in fibroblast cultures from homozygous mutant animals.|
|Mutations Made By|| |
Dr. Sean Baker, Oregon Health Sciences University
While maintaining a live colony, these mice are bred as heterozygotes. Male mice homozygous for the mutation are infertile.
When using the B6.129S2-Pms2tm1Lisk/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #010945 in your Materials and Methods section.
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