STOCK Tg(Pbsn-ERG*)1Vv/J

Stock number: 010929
Research areas: Cancer Research, Reproductive Biology Research

Description

This transgenic line expresses human avian erythroblastosis virus E-26 (v-ets) oncogene related (ERG) in prostrate epithelial cells and exhibits prostatic intraepithelial neoplasia.

Detailed description

Mice that are hemizygous for this transgene are viable and normal in size and express the human avian erythroblastosis virus E-26 (v-ets) oncogene related (ERG) in prostate epithelium. By 5 to 6 months of age, male hemizygotes begin to exhibit prostatic intraepithelial neoplasia (PIN) in the ventral prostate. Although few cells have prominent nuclei, the percentage of cells with nucleomegaly increases with age. Luminal cells in the prostate epithelium accumulate and displace basal cells from the stromal compartment, but do not progress to invasive prostate cancer. This mutant mouse strain may be useful in studies of prostate carcinogenesis.

Development

A transgenic construct containing a modified rat probasin (Pbsn) promoter, beta globin intron, a truncated N-terminal human avian erythroblastosis virus E-26 (v-ets) oncogene related (ERG) cDNA and poly(A) sequences was injected into C57BL/6 X CBA fertilized embryos. Founder line 1 was found to have the highest level of transgene expression. The donating investigator reports that this line was backcrossed to 129S4/SvJaeSor for at least 7 generations prior to arrival at The Jackson Laboratory (see SNP note below). Upon arrival, transgenic mice were bred with 129S1/SvImJ inbred mice (Stock No. 002448) for at least one generation to establish the colony.

A 27 SNP (single nucleotide polymorphism) panel analysis performed by The Jackson Laboratory revealed 9 of 27 markers (on chromsomes 1, 4, 5, 7, 9, 15, 16, and 17) that were not 129S allele-type. These markers are C57BL/6 and CBA allele type; suggesting an incomplete backcrossing onto the 129S background prior to arrival at The Jackson Laboratory.

Allele

Symbol: Tg(Pbsn-ERG*)1Vv
Name: transgene insertion 1, Valeri Vasioukhin
MGI accession ID: MGI:4358243
Generation method: Transgenic
Attributes: Inserted expressed sequence; Humanized sequence
Marker symbol: Tg(Pbsn-ERG*)1Vv
Marker name: transgene insertion 1, Valeri Vasioukhin
Chromosome: UN
Strain of origin: C57BL/6J x CBA
Expressed genes: ERG,ETS transcription factor ERG,human
Molecular note: A transgenic construct containing a rat probasin promoter (Pbsn), beta globin intron, a truncated N-terminal human avian erythroblastosis virus E-26 (v-ets) oncogene related (ERG) cDNA and poly(A) sequences was injected into C57BL/6 X CBA fertilized embryos. Two mouse lines were generated. Founder line 1 had significantly higher levels of the mutant ERG transcript. Truncated ERG protein were higher in Western blots of the ventral prostate with significantly lower levels in dorsolateral and anterior lobes.