Overview

This transgenic line expresses human avian erythroblastosis virus E-26 (v-ets) oncogene related (ERG) in prostrate epithelial cells and exhibits prostatic intraepithelial neoplasia.

Donating Investigator

Valeri Vasioukhin, Fred Hutchinson Cancer Research Center

Genetic overview

Genetic Background	Generation

Tg(Pbsn-ERG*)1Vv

Allele Type
Transgenic (Inserted expressed sequence, Humanized sequence)

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Research Applications

Reproductive Biology Research
Cancer Research

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

Mice that are hemizygous for this transgene are viable and normal in size and express the human avian erythroblastosis virus E-26 (v-ets) oncogene related (ERG) in prostate epithelium. By 5 to 6 months of age, male hemizygotes begin to exhibit prostatic intraepithelial neoplasia (PIN) in the ventral prostate. Although few cells have prominent nuclei, the percentage of cells with nucleomegaly increases with age. Luminal cells in the prostate epithelium accumulate and displace basal cells from the stromal compartment, but do not progress to invasive prostate cancer. This mutant mouse strain may be useful in studies of prostate carcinogenesis.

Development

A transgenic construct containing a modified rat probasin (Pbsn) promoter, beta globin intron, a truncated N-terminal human avian erythroblastosis virus E-26 (v-ets) oncogene related (ERG) cDNA and poly(A) sequences was injected into C57BL/6 X CBA fertilized embryos. Founder line 1 was found to have the highest level of transgene expression. The donating investigator reports that this line was backcrossed to 129S4/SvJaeSor for at least 7 generations prior to arrival at The Jackson Laboratory (see SNP note below). Upon arrival, transgenic mice were bred with 129S1/SvImJ inbred mice (Stock No. 002448) for at least one generation to establish the colony.

A 27 SNP (single nucleotide polymorphism) panel analysis performed by The Jackson Laboratory revealed 9 of 27 markers (on chromsomes 1, 4, 5, 7, 9, 15, 16, and 17) that were not 129S allele-type. These markers are C57BL/6 and CBA allele type; suggesting an incomplete backcrossing onto the 129S background prior to arrival at The Jackson Laboratory.

Expression Data

Expressed Gene	ERG, ETS transcription factor ERG, human
Site of Expression

Control Suggestions

Noncarrier

Approximate Controls

Additional Information

Considerations for Choosing Controls

Selected References

Klezovitch O; Risk M; Coleman I; Lucas JM; Null M; True LD; Nelson PS; Vasioukhin V. 2008. A causal role for ERG in neoplastic transformation of prostate epithelium. Proc Natl Acad Sci U S A 105(6):2105-10PubMed: 18245377 MGI: J:131932

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Genetics

Tg(Pbsn-ERG*)1Vv

Allele Symbol: Tg(Pbsn-ERG*)1Vv

Allele Name	transgene insertion 1, Valeri Vasioukhin
Allele Type	Transgenic (Inserted expressed sequence, Humanized sequence)
Allele Synonym(s)	deltaN-ERG
Gene Symbol and Name	Tg(Pbsn-ERG*)1Vv, transgene insertion 1, Valeri Vasioukhin
Gene Synonym(s)
Promoter	Pbsn, probasin, rat
Expressed Gene	ERG, ETS transcription factor ERG, human
Strain of Origin	C57BL/6J x CBA
Chromosome	UN
Molecular Note	A transgenic construct containing a rat probasin promoter (Pbsn), beta globin intron, a truncated N-terminal human avian erythroblastosis virus E-26 (v-ets) oncogene related (ERG) cDNA and poly(A) sequences was injected into C57BL/6 X CBA fertilized embryos. Two mouse lines were generated. Founder line 1 had significantly higher levels of the mutant ERG transcript. Truncated ERG protein were higher in Western blots of the ventral prostate with significantly lower levels in dorsolateral and anterior lobes.
Mutations Made By	Valeri Vasioukhin, Fred Hutchinson Cancer Research Center

Disease/Phenotype

Disease Terms

Characteristics of this human disease are associated with transgenes and other mutation types in the mouse.

prostate cancer

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Reproductive Biology Research
- Prostate Tumors
Cancer Research
- Increased Tumor Incidence
  - Prostate Tumors

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Tg(Pbsn-ERG)1Vv/0
involves: 129S1/SvImJ C57BL/6J * CBA

endocrine/exocrine gland phenotype

increased prostate gland tumor incidence
- about 50% of mice 2 years of age and older develop prostate tumors
- tumors include adenocarcinomas, sarcomatoid carcinomas (epithelial-mesenchymal transition-like epithelial tumor), and stromal-type tumors

increased prostate gland adenocarcinoma incidence
- adenocarcinomas are composed primarily of luminal cells although small numbers of basal cells are seen close to the margins of the tumors

mortality/aging

premature death
- shorter lifespan than wild-type mice, with mice beginning to die before 20 months of age and none surviving beyond 35 months of age

neoplasm

increased prostate gland tumor incidence
- tumors include adenocarcinomas, sarcomatoid carcinomas (epithelial-mesenchymal transition-like epithelial tumor), and stromal-type tumors
- about 50% of mice 2 years of age and older develop prostate tumors

increased prostate gland adenocarcinoma incidence
- adenocarcinomas are composed primarily of luminal cells although small numbers of basal cells are seen close to the margins of the tumors

reproductive system phenotype

increased prostate gland tumor incidence
- about 50% of mice 2 years of age and older develop prostate tumors
- tumors include adenocarcinomas, sarcomatoid carcinomas (epithelial-mesenchymal transition-like epithelial tumor), and stromal-type tumors

increased prostate gland adenocarcinoma incidence
- adenocarcinomas are composed primarily of luminal cells although small numbers of basal cells are seen close to the margins of the tumors

Genotype: Tg(Pbsn-ERG)1Vv/0
involves: 129/Sv C57BL/6J * CBA

endocrine/exocrine gland phenotype

abnormal prostate gland epithelium morphology
- basal cells are surrounded by abnormally accumulating luminal cells and become displaced away from the stromal cell compartment; direct contact between luminal cells and the basement membrane is observed by electron microscopy
- epithelial compartment exhibits a small but significant increase in proportion of proliferating cells compared to controls
- ratio of basal cells to total prostate epithelial cells is significantly decreased compared to controls

increased prostate intraepithelial neoplasia incidence
- by 5-6 months of age, prostatic intraepithelial neoplasia (PIN) lesions are observed in ventral prostate; cells in these foci display epithelial nuclear pleomorphism and loss of polarization
- number of lesions and cellular pleomorphism increase with mouse age; percentage of cells with nucleolomegaly increases with age

reproductive system phenotype

abnormal prostate gland epithelium morphology
- ratio of basal cells to total prostate epithelial cells is significantly decreased compared to controls
- epithelial compartment exhibits a small but significant increase in proportion of proliferating cells compared to controls
- basal cells are surrounded by abnormally accumulating luminal cells and become displaced away from the stromal cell compartment; direct contact between luminal cells and the basement membrane is observed by electron microscopy

increased prostate intraepithelial neoplasia incidence
- number of lesions and cellular pleomorphism increase with mouse age; percentage of cells with nucleolomegaly increases with age
- by 5-6 months of age, prostatic intraepithelial neoplasia (PIN) lesions are observed in ventral prostate; cells in these foci display epithelial nuclear pleomorphism and loss of polarization

neoplasm

increased prostate intraepithelial neoplasia incidence
- by 5-6 months of age, prostatic intraepithelial neoplasia (PIN) lesions are observed in ventral prostate; cells in these foci display epithelial nuclear pleomorphism and loss of polarization
- number of lesions and cellular pleomorphism increase with mouse age; percentage of cells with nucleolomegaly increases with age

References

Klezovitch O; Risk M; Coleman I; Lucas JM; Null M; True LD; Nelson PS; Vasioukhin V. 2008. A causal role for ERG in neoplastic transformation of prostate epithelium. Proc Natl Acad Sci U S A 105(6):2105-10PubMed: 18245377 MGI: J:131932

Additional - Tg(Pbsn-ERG*)1Vv related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Genotyping resources and troubleshooting
Breeding Considerations

While maintaining a live colony, hemizygous mice may be bred to wildtype (noncarrier) siblings.
- Additional Breeding and Husbandry Support
Citation
When using the STOCK Tg(Pbsn-ERG*)1Vv/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #010929 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

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Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
> >	Hemizygous or Non carrier for Tg(Pbsn-ERG*)1Vv

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

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Additional Use Restrictions Apply

Use of MICE by companies or for-profit entities requires a license.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Approximate Controls

Additional Information

Selected References

Tg(Pbsn-ERG*)1Vv

Allele Symbol: Tg(Pbsn-ERG*)1Vv

Disease Terms

Characteristics of this human disease are associated with transgenes and other mutation types in the mouse.

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Tg(Pbsn-ERG*)1Vv/0involves: 129S1/SvImJ * C57BL/6J * CBA

endocrine/exocrine gland phenotype

mortality/aging

neoplasm

reproductive system phenotype

Genotype: Tg(Pbsn-ERG*)1Vv/0involves: 129/Sv * C57BL/6J * CBA

endocrine/exocrine gland phenotype

reproductive system phenotype

neoplasm

References

Additional - Tg(Pbsn-ERG*)1Vv related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

Genotype: Tg(Pbsn-ERG)1Vv/0
involves: 129S1/SvImJ C57BL/6J * CBA

Genotype: Tg(Pbsn-ERG)1Vv/0
involves: 129/Sv C57BL/6J * CBA