This transgenic line expresses human avian erythroblastosis virus E-26 (v-ets) oncogene related (ERG) in prostrate epithelial cells and exhibits prostatic intraepithelial neoplasia.
Valeri Vasioukhin, Fred Hutchinson Cancer Research Center
Genetic Background | Generation |
---|---|
|
Allele Type |
---|
Transgenic (Inserted expressed sequence, Humanized sequence) |
Mice that are hemizygous for this transgene are viable and normal in size and express the human avian erythroblastosis virus E-26 (v-ets) oncogene related (ERG) in prostate epithelium. By 5 to 6 months of age, male hemizygotes begin to exhibit prostatic intraepithelial neoplasia (PIN) in the ventral prostate. Although few cells have prominent nuclei, the percentage of cells with nucleomegaly increases with age. Luminal cells in the prostate epithelium accumulate and displace basal cells from the stromal compartment, but do not progress to invasive prostate cancer. This mutant mouse strain may be useful in studies of prostate carcinogenesis.
A transgenic construct containing a modified rat probasin (Pbsn) promoter, beta globin intron, a truncated N-terminal human avian erythroblastosis virus E-26 (v-ets) oncogene related (ERG) cDNA and poly(A) sequences was injected into C57BL/6 X CBA fertilized embryos. Founder line 1 was found to have the highest level of transgene expression. The donating investigator reports that this line was backcrossed to 129S4/SvJaeSor for at least 7 generations prior to arrival at The Jackson Laboratory (see SNP note below). Upon arrival, transgenic mice were bred with 129S1/SvImJ inbred mice (Stock No. 002448) for at least one generation to establish the colony.
A 27 SNP (single nucleotide polymorphism) panel analysis performed by The Jackson Laboratory revealed 9 of 27 markers (on chromsomes 1, 4, 5, 7, 9, 15, 16, and 17) that were not 129S allele-type. These markers are C57BL/6 and CBA allele type; suggesting an incomplete backcrossing onto the 129S background prior to arrival at The Jackson Laboratory.
Expressed Gene | ERG, ETS transcription factor ERG, human |
---|---|
Site of Expression |
Allele Name | transgene insertion 1, Valeri Vasioukhin |
---|---|
Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | deltaN-ERG |
Gene Symbol and Name | Tg(Pbsn-ERG*)1Vv, transgene insertion 1, Valeri Vasioukhin |
Gene Synonym(s) | |
Promoter | Pbsn, probasin, rat |
Expressed Gene | ERG, ETS transcription factor ERG, human |
Strain of Origin | C57BL/6J x CBA |
Chromosome | UN |
Molecular Note | A transgenic construct containing a rat probasin promoter (Pbsn), beta globin intron, a truncated N-terminal human avian erythroblastosis virus E-26 (v-ets) oncogene related (ERG) cDNA and poly(A) sequences was injected into C57BL/6 X CBA fertilized embryos. Two mouse lines were generated. Founder line 1 had significantly higher levels of the mutant ERG transcript. Truncated ERG protein were higher in Western blots of the ventral prostate with significantly lower levels in dorsolateral and anterior lobes. |
Mutations Made By | Valeri Vasioukhin, Fred Hutchinson Cancer Research Center |
While maintaining a live colony, hemizygous mice may be bred to wildtype (noncarrier) siblings.
When using the STOCK Tg(Pbsn-ERG*)1Vv/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #010929 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous or Non carrier for Tg(Pbsn-ERG*)1Vv |
Frozen Mouse Embryo | STOCK Tg(Pbsn-ERG*)1Vv/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(Pbsn-ERG*)1Vv/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(Pbsn-ERG*)1Vv/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | STOCK Tg(Pbsn-ERG*)1Vv/J Frozen Embryos | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.