This chemically induced mutation of the seizure threshold 2 (Szt2) gene displays an low threshold for kindling induced seizures and may be useful in the studies of seizure susceptibility.
Wayne Frankel, JAX
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Chemically induced (ENU) | Szt2 | seizure threshold 2 |
Mice that are homozygous for the mutation are viable, fertile, normal in size and do not display any electroencephalograhic, neurosensory or motor abnormalities. Although mice do not exhibit spontaneous seizures, non-invasive kindling produces an earlier latency to first minimal clonic seizure than C57BL/6J controls (4 tests vs. 7-8 tests). This mutant mouse strain may be useful in studies of epilepsy susceptibility.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
This mutation was generated by ethylnitrosourea (ENU) mutagenesis in C57BL/6J males. Descendants of the mutagenized males were selected for low electroconvulsive (seizure) threshold (ECT). A point mutation (T to A) in the exon 32 splice site donor results in inclusion of intron 32. Mice were backcrossed to 129S1/SvImJ for ten generations before cryopreservation in 2010.
Allele Name | mutation 1, Wayne N Frankel |
---|---|
Allele Type | Chemically induced (ENU) |
Allele Synonym(s) | |
Gene Symbol and Name | Szt2, seizure threshold 2 |
Gene Synonym(s) | |
Strain of Origin | C57BL/6J |
Chromosome | 4 |
Molecular Note | A T to A mutation was induced by ENU in the exon 32 splice site donor resulting in inclusion of intron 32 in the mutant transcript. Northern blot analysis indicates about a 4 fold increase in mRNA expression in whole brains from homozygous mice. |
When using the 129S1.B6-Szt2m1Frk/Frk mouse strain in a publication, please cite the originating article(s) and include JAX stock #010918 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Szt2<m1Frk> |
Frozen Mouse Embryo | 129S1.B6-Szt2<m1Frk>/Frk | $2595.00 |
Frozen Mouse Embryo | 129S1.B6-Szt2<m1Frk>/Frk | $2595.00 |
Frozen Mouse Embryo | 129S1.B6-Szt2<m1Frk>/Frk | $3373.50 |
Frozen Mouse Embryo | 129S1.B6-Szt2<m1Frk>/Frk | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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