Mice harboring the pEμSR-IL-14α transgene express hemagglutinin-tagged human IL-14α (TXLNA or taxilin alpha) predominantly in the B lymphocyte compartment under the direction of Eμ immunoglobulin heavy chain enhancer/SV40 promoter regions. These pEμSR-IL-14α transgenic mice may be useful in studying both autoimmune and B cell lymphoma diseases such as systemic lupus erythematosus (SLE) and Sjogren's syndrome.
Julian L Ambrus, Jr, SUNY at Buffalo School of Medicine
Mice harboring the pEμSR-IL-14α transgene are viable and fertile, with expression of hemagglutinin-tagged human IL-14α (TXLNA or taxilin alpha) directed predominantly to the B lymphocyte compartment by the Eμ immunoglobulin heavy chain enhancer/SV40 promoter regions (from the pEμSR vector). Human IL-14α expression levels from the transgene are similar to mouse IL-14α expression levels observed in activated T cells/B cells. Transgene expression results in a phenotype with characteristics of systemic lupus erythematosus (SLE) and Sjogren's syndrome. Specifically, transgenic mice exhibit hypergammaglobulinemia (enhanced responses to T-dependent and T-independent antigens), autoantibodies, sialadenitis (infiltration of the parotid glands with lymphocytes), and mild immune-complex mediated nephritis with glomerular IgM deposition. In addition, almost all aged IL-14α-transgenic mice develop large B cell lymphoma (CD5+ B cell lymphoma) similar to the tumors seen in elderly patients with SLE and Sjogren's syndrome.
The pEμSR-IL-14α transgene was designed with the Eμ immunoglobulin heavy chain enhancer/SV40 promoter regions (from the pEμSR vector) upstream of a hemagglutinin-tagged human IL-14α (TXLNA or taxilin alpha) cDNA sequence (human IL-14α is a cytokine encoded by the plus strand of the IL-14 gene using exons 3-10). This transgene was injected into C3H oocytes fertilized with C57BL/6J sperm. Founder mice were bred to C57BL/6J mice to establish the colony. The donating investigator reported that these pEuSR-IL-14α transgenic mice (found to harbor the transgene predominantly on chromosome 18) were backcrossed to C57BL/6J mice for more than eight generations (see SNP note below). Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, all 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.
|Expressed Gene||TXLNA, taxilin alpha, human|
|Site of Expression|
|Allele Name||transgene insertion 1, Julian Ambrus Jr|
|Allele Type||Transgenic (Inserted expressed sequence, Humanized sequence)|
|Allele Synonym(s)||IL-14alpha Tg; pEuSR-IL-14alpha|
|Gene Symbol and Name||Tg(Emu-TXLNA)1Amjr, transgene insertion 1, Julian Ambrus Jr|
|Promoter||SV40, E mu enhancer, SV40|
|Expressed Gene||TXLNA, taxilin alpha, human|
|Strain of Origin||(C3H x C57BL/6J)F1|
|Molecular Note||The pEmuSR-IL-14alpha transgene was designed with the Emu immunoglobulin heavy chain enhancer/SV40 promoter regions (from the pEmuSR vector) upstream of a hemagglutinin-tagged human IL-14alpha (TXLNA or taxilin alpha) cDNA sequence (human IL-14alpha is a cytokine encoded by the plus strand of the IL-14 gene using exons 3-10). FISH analysis showed positive immunofluorescence on chromosome 18.|
|Mutations Made By|| |
Julian Ambrus, Jr, SUNY at Buffalo School of Medicine
When maintaining a live colony, hemizygous mice may be bred to wildtype (noncarrier) siblings or to C57BL/6J inbred mice (Stock No. 000664).
When using the B6.Cg-Tg(Emu-TXLNA)1Amjr/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #010914 in your Materials and Methods section.
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCT(S)" means biological materials supplied by JACKSON, and their derivatives. "SERVICES" means projects conducted by JACKSON for other parties that may include but are not limited to the use of MICE or PRODUCTS. "RECIPIENT" means each recipient of MICE, PRODUCTS, or SERVICES provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE, PRODUCTS or SERVICES from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON’s prior written authorization.
MICE, PRODUCTS AND SERVICES ARE PROVIDED "AS IS". JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of, PRODUCTS or SERVICES, JACKSON will, at its option, provide credit or replacement for the PRODUCT received or the SERVICES provided; JACKSON makes no other representations and this shall be the exclusive remedy of the purchaser. Please note specific policy for live mice.
Consistent with the requirement for a written understanding regarding animal care and use, the JACKSON Animal Care and Use Committee will review the animal care and use protocol(s) associated with any SERVICES to be performed at JACKSON, and JACKSON shall have ultimate responsibility and authority for the care of animals while on site or in JACKSON custody.
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE, PRODUCTS or SERVICES are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or SERVICES shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or SERVICES by JACKSON.