Overview

Male mice that are homozygous for this targeted mutation of Per3, period homolog 2 (Drosophila), exhibit a shorter free running period when housed in constant darkness. This mutant mouse strain may be useful in studies of circadian rhythm and sleep patterns.

Donating Investigator

David R. Weaver, Univ of Massachusetts Medical School

Genetic overview

Genetic Background	Generation

Per3^tm1Drw

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Per3	period circadian clock 3

View Genetics

Research Applications

Neurobiology Research

View All Research Applications

Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

View Price List

Details

Detailed Description

Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (protein) is detected by Western blot analysis of whole brain minus cerebellum and hypothalamus, whole hypothalamus, and anterior hypothalamus containing SCN, from homozygotes. Immunoprecipitation analysis of brain lysates from homozygotes does not detect any gene product (protein). Abberant gene products (mRNA) are detected by Northern blot analysis and RT-PCR, and have rhythmic expression. Under conditions of constant darkness, homozygous males exhibit a shorter free running period. A subset of mice exhibit progressive shortening of the period length in extended constant darkness. This mutant mouse strain may be useful in studies of circadian rhythm and sleep patterns.

Please note: Mice derived from 129S-Per3^tm1Drw/J are reported to a carry a spontaneous deletion in exon 3 of the Mfrp gene (174delG or rdx) that causes photoreceptor degeneration and eventual retinal pigment epithelium. It is unknown if the Per3 KO carries the mutation. (IOVS 2011 52(10):7256)

Development

A targeting vector containing a PGKneo cassette was used to disrupt exon 3 and part of exon 4. The construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric male animals were crossed to female 129S4/SvJae mice. Heterozygotes were intercrossed to generate homozygotes. Upon arrival at The Jackson Laboratory, mutant mice were bred with 129S1/SvImJ inbred mice (Stock No. 002448) for at least one generation to establish the colony.

Control Suggestions

Wild-type from the colony

Approximate Controls

Additional Information

Considerations for Choosing Controls

Selected References

Shearman LP; Jin X; Lee C; Reppert SM; Weaver DR. 2000. Targeted disruption of the mPer3 gene: subtle effects on circadian clock function. Mol Cell Biol 20(17):6269-75PubMed: 10938103 MGI: J:63933

View All References

Genetics

Per3^tm1Drw

Allele Symbol: Per3^tm1Drw

Allele Name	targeted mutation 1, David R Weaver
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	mPER3-deficient
Gene Symbol and Name	Per3, period circadian clock 3
Gene Synonym(s)
Strain of Origin	129S4/SvJae
Chromosome	4
Molecular Note	The gene was disrupted by replacement of exon 3 and part of exon 4 (encoding amino acids 92-154) with a PGK-neo cassette via homologous recombination. Introduction of PGK-neo introduces stop codons into all 3 reading frames. Western blot analysis of brain lysates from homozygous mutant animals using antibodies directed against the C-terminus of the protein confirmed the absence of protein expression. Immunoprecipitation assays also confirmed the absence of protein product. Northern blot and RT-PCR analysis detected mutant transcripts containing neo and lacking exon 3 and part of exon 4 in brain and skeletal muscles from homozygous mutants.
Mutations Made By	Shin Yamazaki, Vanderbilt University

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Neurobiology Research
- Circadian Rhythms

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Per3^tm1Drw/Per3^tm1Drw
involves: 129S4/SvJae * C57BL/6

behavior/neurological phenotype

behavioral despair
- mice exhibit an increased duration of immobility in forced swim test under 4 hr light/20 hr dark, but not under 12 hr light/12 hr dark
- mice exhibit an increased duration of immobility in tail suspension test under 12 hr light/12 hr dark; duration of immobility is intensified under 4 hr light/20 hr dark
- mice ingest less sucrose solution than controls
- treatment with anti-depressant imipramine decreases duration of immobility

The following phenotype relates to a compound genotype created using this strain

Genotype: Per3^tm1Drw/Per3^tm1Drw
involves: 129S4/SvJae

behavior/neurological phenotype

abnormal sleep pattern
- during the 12-hour recovery after 6 hours of prolonged waking, mice exhibit less waking than similarly treated wild-type mice

shortened circadian behavior period
- homozygous male mice exhibited a shorter free running period when exposed to constant light; a subset of these mice continued to exhibit a shortening of period length progressive with time

nervous system phenotype

abnormal brain wave pattern
- recovery after 6 hours of prolonged waking, mice exhibit an increase in delta power lasting the first 6 hours of recovery sleep period compared with wild-type mice in which it lasts 3 hours

References

Shearman LP; Jin X; Lee C; Reppert SM; Weaver DR. 2000. Targeted disruption of the mPer3 gene: subtle effects on circadian clock function. Mol Cell Biol 20(17):6269-75PubMed: 10938103 MGI: J:63933

Additional - Per3^tm1Drw related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Per3
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, these mice can be bred as homozygotes. These mice may carry a spontaneous mutation (rdx) that causes retinal degeneration.
- Additional Breeding and Husbandry Support
Citation
When using the 129S-Per3^tm1Drw/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #010833 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Heterozygous or wildytpe for Per3<tm1Drw>

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

Use of MICE by companies or for-profit entities requires a license prior to shipping.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCT(S)" means biological materials supplied by JACKSON, and their derivatives. "SERVICES" means projects conducted by JACKSON for other parties that may include but are not limited to the use of MICE or PRODUCTS. "RECIPIENT" means each recipient of MICE, PRODUCTS, or SERVICES provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE, PRODUCTS or SERVICES from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON’s prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED "AS IS". JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

Credit for PRODUCTS or SERVICES

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of, PRODUCTS or SERVICES, JACKSON will, at its option, provide credit or replacement for the PRODUCT received or the SERVICES provided; JACKSON makes no other representations and this shall be the exclusive remedy of the purchaser. Please note specific policy for live mice.

Animal Care and Use for SERVICES

Consistent with the requirement for a written understanding regarding animal care and use, the JACKSON Animal Care and Use Committee will review the animal care and use protocol(s) associated with any SERVICES to be performed at JACKSON, and JACKSON shall have ultimate responsibility and authority for the care of animals while on site or in JACKSON custody.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE, PRODUCTS or SERVICES are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or SERVICES shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or SERVICES by JACKSON.

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Approximate Controls

Additional Information

Selected References

Per3tm1Drw

Allele Symbol: Per3tm1Drw

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Per3tm1Drw/Per3tm1Drwinvolves: 129S4/SvJae * C57BL/6

behavior/neurological phenotype

Genotype: Per3tm1Drw/Per3tm1Drwinvolves: 129S4/SvJae

behavior/neurological phenotype

nervous system phenotype

References

Additional - Per3tm1Drw related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Per3^tm1Drw

Allele Symbol: Per3^tm1Drw

Genotype: Per3^tm1Drw/Per3^tm1Drw
involves: 129S4/SvJae * C57BL/6

Genotype: Per3^tm1Drw/Per3^tm1Drw
involves: 129S4/SvJae

Additional - Per3^tm1Drw related