Mice that are homozygous for this targeted mutation of Per2, period homolog 2 (Drosophila), exhibit a short circadian period initially when housed in constant darkness, and then exhibit gradual loss of rhythmicity. Homozygotes exhibit phase shift responses to light. This mutant mouse strain may be useful in studies of circadian rhythm and sleep patterns.
David R. Weaver, Univ of Massachusetts Medical School
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Per2 | period circadian clock 2 |
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Abberant gene products (mRNA) are detected by RT-PCR and RACE analysis of brain total RNA. No gene product (protein) is detected in homozygous mutant mice by immunohistochemical staining of superchiasmatic nuclei over a 24 hour period. Western blots of liver reveal a deletion mutant protein product is produced. When housed in constant darkness, homozygotes have a short circadian period initially, and then exhibit gradual loss of rhythmicity. Homozygotes exhibit phase shift responses to light. This mutant mouse strain may be useful in studies of circadian rhythm and sleep patterns.
A targeting vector containing a PGKneo cassette was used to disrupt exon 5 and a portion of exon 6. The construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric male animals were crossed to 129S4/SvJae female mice. Heterozygotes were intercrossed to generate homozygotes. Upon arrival at The Jackson Laboratory, mutant mice were bred with 129S1/SvImJ inbred mice (Stock No. 002448) for at least one generation to establish the colony.
Allele Name | targeted mutation 1, David R Weaver |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | mPer2ldc; Per2-; Per2m |
Gene Symbol and Name | Per2, period circadian clock 2 |
Gene Synonym(s) | |
Strain of Origin | 129S4/SvJae |
Chromosome | 1 |
Molecular Note | Exon 5 and a portion of exon 6 were replaced with a neomycin selection cassette. Several transcript forms produced from the targeted allele were identified in homozygous mutant mice via RT-PCR and RACE analyses of brain RNA. The different transcripts involved aberrant splicing of exon 4 to a pseudoexon in intron 4, to a cryptic splice site within neo, and to exon 7. In spite of the transcripts having in-frame regions, normal protein was undetected in by immunohistochemical staining of superchiasmatic nuclei over a 24 hour period. |
Mutations Made By | Shin Yamazaki, Vanderbilt University |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the 129S-Per2tm1Drw/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #010832 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Per2<tm1Drw> |
Frozen Mouse Embryo | 129S-Per2<tm1Drw>/J | $2595.00 |
Frozen Mouse Embryo | 129S-Per2<tm1Drw>/J | $2595.00 |
Frozen Mouse Embryo | 129S-Per2<tm1Drw>/J | $3373.50 |
Frozen Mouse Embryo | 129S-Per2<tm1Drw>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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