Mice that are homozygous for this targeted mutation of Per2, period homolog 2 (Drosophila), exhibit a short circadian period initially when housed in constant darkness, and then exhibit gradual loss of rhythmicity. Homozygotes exhibit phase shift responses to light. This mutant mouse strain may be useful in studies of circadian rhythm and sleep patterns.
David R. Weaver, Univ of Massachusetts Medical School
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Abberant gene products (mRNA) are detected by RT-PCR and RACE analysis of brain total RNA. No gene product (protein) is detected in homozygous mutant mice by immunohistochemical staining of superchiasmatic nuclei over a 24 hour period. Western blots of liver reveal a deletion mutant protein product is produced. When housed in constant darkness, homozygotes have a short circadian period initially, and then exhibit gradual loss of rhythmicity. Homozygotes exhibit phase shift responses to light. This mutant mouse strain may be useful in studies of circadian rhythm and sleep patterns.
A targeting vector containing a PGKneo cassette was used to disrupt exon 5 and a portion of exon 6. The construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric male animals were crossed to 129S4/SvJae female mice. Heterozygotes were intercrossed to generate homozygotes. Upon arrival at The Jackson Laboratory, mutant mice were bred with 129S1/SvImJ inbred mice (Stock No. 002448) for at least one generation to establish the colony.
|Allele Name||targeted mutation 1, David R Weaver|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||mPer2ldc; Per2-; Per2m|
|Gene Symbol and Name||Per2, period circadian clock 2|
|Strain of Origin||129S4/SvJae|
|Molecular Note||Exon 5 and a portion of exon 6 were replaced with a neomycin selection cassette. Several transcript forms produced from the targeted allele were identified in homozygous mutant mice via RT-PCR and RACE analyses of brain RNA. The different transcripts involved aberrant splicing of exon 4 to a pseudoexon in intron 4, to a cryptic splice site within neo, and to exon 7. In spite of the transcripts having in-frame regions, normal protein was undetected in by immunohistochemical staining of superchiasmatic nuclei over a 24 hour period.|
|Mutations Made By|| |
Shin Yamazaki, Vanderbilt University
When maintaining a live colony, these mice can be bred as homozygotes.
When using the 129S-Per2tm1Drw/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #010832 in your Materials and Methods section.
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCT(S)" means biological materials supplied by JACKSON, and their derivatives. "SERVICES" means projects conducted by JACKSON for other parties that may include but are not limited to the use of MICE or PRODUCTS. "RECIPIENT" means each recipient of MICE, PRODUCTS, or SERVICES provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE, PRODUCTS or SERVICES from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON’s prior written authorization.
MICE, PRODUCTS AND SERVICES ARE PROVIDED "AS IS". JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of, PRODUCTS or SERVICES, JACKSON will, at its option, provide credit or replacement for the PRODUCT received or the SERVICES provided; JACKSON makes no other representations and this shall be the exclusive remedy of the purchaser. Please note specific policy for live mice.
Consistent with the requirement for a written understanding regarding animal care and use, the JACKSON Animal Care and Use Committee will review the animal care and use protocol(s) associated with any SERVICES to be performed at JACKSON, and JACKSON shall have ultimate responsibility and authority for the care of animals while on site or in JACKSON custody.
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE, PRODUCTS or SERVICES are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or SERVICES shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or SERVICES by JACKSON.