Overview

Mice that are homozygous for this targeted mutation of Per2, period homolog 2 (Drosophila), exhibit a short circadian period initially when housed in constant darkness, and then exhibit gradual loss of rhythmicity. Homozygotes exhibit phase shift responses to light. This mutant mouse strain may be useful in studies of circadian rhythm and sleep patterns.

Donating Investigator

David R. Weaver, Univ of Massachusetts Medical School

Genetic overview

Genetic Background	Generation

Per2^tm1Drw

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Per2	period circadian clock 2

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Research Applications

Neurobiology Research

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Abberant gene products (mRNA) are detected by RT-PCR and RACE analysis of brain total RNA. No gene product (protein) is detected in homozygous mutant mice by immunohistochemical staining of superchiasmatic nuclei over a 24 hour period. Western blots of liver reveal a deletion mutant protein product is produced. When housed in constant darkness, homozygotes have a short circadian period initially, and then exhibit gradual loss of rhythmicity. Homozygotes exhibit phase shift responses to light. This mutant mouse strain may be useful in studies of circadian rhythm and sleep patterns.

Development

A targeting vector containing a PGKneo cassette was used to disrupt exon 5 and a portion of exon 6. The construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric male animals were crossed to 129S4/SvJae female mice. Heterozygotes were intercrossed to generate homozygotes. Upon arrival at The Jackson Laboratory, mutant mice were bred with 129S1/SvImJ inbred mice (Stock No. 002448) for at least one generation to establish the colony.

Control Suggestions

Approximate Controls

Additional Information

Considerations for Choosing Controls

Selected References

Bae K; Jin X; Maywood ES; Hastings MH; Reppert SM; Weaver DR. 2001. Differential Functions of mPer1, mPer2, and mPer3 in the SCN Circadian Clock. Neuron 30(2):525-36PubMed: 11395012 MGI: J:69626

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Genetics

Per2^tm1Drw

Allele Symbol: Per2^tm1Drw

Allele Name	targeted mutation 1, David R Weaver
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	targeted mutation 1, David R Weaver; Per2^tm1Drw
Gene Symbol and Name	Per2, period circadian clock 2
Gene Synonym(s)	mKIAA0347; FASPS1; rPER2; FASPS; mPer2
Strain of Origin	129S4/SvJae
Chromosome	1
Molecular Note	Exon 5 and a portion of exon 6 were replaced with a neomycin selection cassette. Several transcript forms produced from the targeted allele were identified in homozygous mutant mice via RT-PCR and RACE analyses of brain RNA. The different transcripts involved aberrant splicing of exon 4 to a pseudoexon in intron 4, to a cryptic splice site within neo, and to exon 7. In spite of the transcripts having in-frame regions, normal protein was undetected in by immunohistochemical staining of superchiasmatic nuclei over a 24 hour period.
Mutations Made By	Shin Yamazaki, Vanderbilt University

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Neurobiology Research
- Circadian Rhythms

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Per2^tm1Drw/Per2^tm1Drw
B6.129-Per2/J

cardiovascular system phenotype

abnormal blood vessel morphology
- mice show a 25% reduction in the number of vessels in the sciatic nerve indicating that the network of vasa nervorum is reduced

abnormal retinal vasculature morphology
- 40% increase in acellular capillaries in the retina indicating retinal vascular damage

vision/eye phenotype

abnormal retina morphology
- dissociation of tight junctions and adherens junctional integrity in the retina
- increase in retinal permeability

abnormal retinal vasculature morphology
- 40% increase in acellular capillaries in the retina indicating retinal vascular damage

cellular phenotype

decreased hematopoietic stem cell proliferation
- BMPCs show a 3-fold decrease in proliferation

hematopoietic system phenotype

abnormal bone marrow cell morphology/development
- mice exhibit bone marrow neuropathy, with a decrease in tyrosine hydroxylase-positive and neurofilament 200-positive nerve processes at 4 and 12 months of age

abnormal hematopoietic stem cell physiology
- BMPCs show decreased colony formation and NO levels

decreased hematopoietic stem cell proliferation
- BMPCs show a 3-fold decrease in proliferation

increased hematopoietic stem cell number
- 4 month old mice show a 2-fold increase in lin- sca1+ c-Kit+ (LSK) cells within the bone marrow, indicating a dysfunction in bone marrow progenitor cell (BMPC) release from bone marrow

homeostasis/metabolism phenotype

improved glucose tolerance
- Normal - mice are normoglycemic and show better recovery after glucose challenge than wild-type mice

nervous system phenotype

abnormal nervous system morphology
- mice exhibit bone marrow neuropathy, with a decrease in tyrosine hydroxylase-positive and neurofilament 200-positive nerve processes at 4 and 12 months of age, respectively

The following phenotype relates to a compound genotype created using this strain

Genotype: Per2^tm1Drw/Per2^tm1Drw
involves: 129

behavior/neurological phenotype

abnormal sleep pattern
- at midday, mice exhibit less REM sleep than wild-type mice
- at the midday 3-hour period, mice wake more than wild-type mice
- during the 12-hour recovery after 6 hours of prolonged waking, mice exhibit less waking and more short wave sleep compared to similarly treated wild-type mice
- mice are awake more during the mid-third of the light period compared with wild-type mice

arrhythmic circadian behavior persistence
- all mice become arrhythmic when transferred into dark-dark conditions although at different duration and extent of rhythm persistence

homeostasis/metabolism phenotype

abnormal circadian temperature homeostasis
- the acrophase of core temperature is phase-advanced compared to in wild-type mice

References

Bae K; Jin X; Maywood ES; Hastings MH; Reppert SM; Weaver DR. 2001. Differential Functions of mPer1, mPer2, and mPer3 in the SCN Circadian Clock. Neuron 30(2):525-36PubMed: 11395012 MGI: J:69626

Additional - Per2^tm1Drw related

Technical Support

Contact Technical Support

Genotyping Protocols
- Standard PCR: Per2^tm1Drw
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, these mice can be bred as homozygotes.
- Additional Breeding and Husbandry Support
Citation
When using the 129S-Per2^tm1Drw/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #010832 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous for Per2<tm1Drw>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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General Terms and Conditions

Questions about Terms of Use

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Licensing Information

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Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Approximate Controls

Additional Information

Selected References

Per2tm1Drw

Allele Symbol: Per2tm1Drw

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Per2tm1Drw/Per2tm1DrwB6.129-Per2/J

cardiovascular system phenotype

vision/eye phenotype

cellular phenotype

hematopoietic system phenotype

homeostasis/metabolism phenotype

nervous system phenotype

Genotype: Per2tm1Drw/Per2tm1Drwinvolves: 129

behavior/neurological phenotype

homeostasis/metabolism phenotype

References

Additional - Per2tm1Drw related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Per2^tm1Drw

Allele Symbol: Per2^tm1Drw

Genotype: Per2^tm1Drw/Per2^tm1Drw
B6.129-Per2/J

Genotype: Per2^tm1Drw/Per2^tm1Drw
involves: 129

Additional - Per2^tm1Drw related