Overview

Also Known As:RCE:FRT

These RCE:FRT mice harbor the R26R CAG-boosted EGFP (RCE) reporter allele with a FRT-flanked STOP cassette upstream of the enhanced green fluorescent protein (EGFP) gene. After removal of the FRT-flanked STOP cassette by Flp-mediated recombination, EGFP reporter expression is directed to the cells/tissues dependent upon expression of the promoter driving FLP recombinase.

Donating Investigator

Gord Fishell, Harvard Medical School

Genetic overview

Genetic Background	Generation
	?+pN2F2 (2021-02-16 00:00:00)

Gt(ROSA)26Sor^{tm1.2(CAG-EGFP)Fsh}

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Gt(ROSA)26Sor	gene trap ROSA 26, Philippe Soriano

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Research Applications

Research Tools
Neurobiology Research

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Details

Detailed Description

Mice homozygous for the R26R CAG-boosted EGFP (RCE) reporter allele harboring an FRT-flanked STOP cassette (RCE:FRT) are viable and fertile. Under control of the endogenous Gt(ROSA)26Sor promoter/enhancer regions and the CMV-IE enhancer/chicken beta-actin/rabbit beta-globin (CAG) hybrid promoter, widespread expression of enhanced green fluorescent protein (EGFP) is prevented by the upstream FRT-flanked STOP cassette. After removal of the FRT-flanked STOP cassette by Flp-mediated recombination, EGFP reporter expression is directed to the cells/tissues dependent upon the expression pattern of the promoter driving FLP recombinase.

Development

The R26R CAG-boosted EGFP (RCE) reporter targeting vector was designed with (from 5' to 3') the CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG; from the pCAGGS vector), a splice acceptor site, a loxP-flanked transcriptional STOP cassette (PGK-Neo-polyA and 3x-polyA), an FRT-flanked modified pBS302 STOP cassette, an enhanced green fluorescent protein (EGFP) sequence, and two AttP-polyA sequences. This entire construct was inserted into the first exon of the Gt(ROSA)26Sor locus via electroporation into 129S6/SvEvTac-derived W4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts, and chimeric mice were bred with C57BL/6 mice to generate the RCE:dual colony. RCE:dual mice were then bred with a germline Cre-expressing strain (unspecified genetic background) to delete the loxP-flanked transcriptional STOP cassette. The resulting RCE:FRT offspring were mated with outbred Swiss Webster mice for many generations (selectively removing the Cre-expressing transgene), and subsequently maintained as homozygotes prior to arrival at The Jackson Laboratory. Upon arrival, mutant mice were bred to C57BL/6J inbred mice for at least one generation to establish the RCE:FRT colony.

Control Suggestions

None Available

Additional Information

Considerations for Choosing Controls

Selected References

Sousa VH; Miyoshi G; Hjerling-Leffler J; Karayannis T; Fishell G. 2009. Characterization of Nkx6-2-derived neocortical interneuron lineages. Cereb Cortex 19 Suppl 1:i1-10PubMed: 19363146 MGI: J:155164

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Genetics

Gt(ROSA)26Sor^{tm1.2(CAG-EGFP)Fsh}

Allele Symbol: Gt(ROSA)26Sor^{tm1.2(CAG-EGFP)Fsh}

Allele Name	targeted mutation 1.2, Gordon Fishell
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	RCE:FRT
Gene Symbol and Name	Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano
Gene Synonym(s)
Site of Expression	EGFP will be expressed in cells where promoters driving Flp recombinase are expressed.
Strain of Origin	129S6/SvEvTac
Chromosome	6
Molecular Note	Cre mediated recombination removes the loxP flanked STOP cassette from Gt(ROSA)26Sor^{tm1(CAG-EGFP)Fsh}. After removal of the FRT-flanked STOP cassette by Flp-mediated recombination, EGFP reporter expression is directed to the cells/tissues dependent upon the expression pattern of the promoter driving FLP recombinase.
Mutations Made By	Gord Fishell, Harvard Medical School

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- FLP-FRT System
  - FRT-flanked Sequences: Test/Reporter
  - FRT-flanked Sequences
- Fluorescent Proteins
- Genetics Research
  - Tissue/Cell Markers
  - Mutagenesis and Transgenesis
Neurobiology Research
- Fluorescent protein expression in neural tissue

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Gt(ROSA)26Sor^{tm1.2(CAG-EGFP)Fsh}/Gt(ROSA)26Sor⁺
involves: 129S6/SvEvTac * C57BL/6 * Swiss Webster

mammalian phenotype

no phenotypic analysis

References

Sousa VH; Miyoshi G; Hjerling-Leffler J; Karayannis T; Fishell G. 2009. Characterization of Nkx6-2-derived neocortical interneuron lineages. Cereb Cortex 19 Suppl 1:i1-10PubMed: 19363146 MGI: J:155164

Additional - Gt(ROSA)26Sor^{tm1.2(CAG-EGFP)Fsh} related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Probe:Gt(Rosa)26Sor(CAG) alternate 2
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, heterozygous mice may be bred together or to wildtype mice from the colony. Homozygous mice may also be bred together.
- Additional Breeding and Husbandry Support
Mating System
- Homozygote x Homozygote
Citation
When using the RCE:FRT mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #32038 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

AX18 (Maximum)

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

See MMRRC for Additional Conditions of Distribution

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:RCE:FRT

Donating Investigator

Genetic overview

Research Applications

Detailed Description

Development

Control Suggestions

Additional Information

Selected References

Gt(ROSA)26Sortm1.2(CAG-EGFP)Fsh

Allele Symbol: Gt(ROSA)26Sortm1.2(CAG-EGFP)Fsh

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Gt(ROSA)26Sortm1.2(CAG-EGFP)Fsh/Gt(ROSA)26Sor+involves: 129S6/SvEvTac * C57BL/6 * Swiss Webster

mammalian phenotype

References

Additional - Gt(ROSA)26Sortm1.2(CAG-EGFP)Fsh related

Genotyping Protocols

Breeding Considerations

Mating System

Citation

Animal Health Reports

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

Gt(ROSA)26Sor^{tm1.2(CAG-EGFP)Fsh}

Allele Symbol: Gt(ROSA)26Sor^{tm1.2(CAG-EGFP)Fsh}

Genotype: Gt(ROSA)26Sor^{tm1.2(CAG-EGFP)Fsh}/Gt(ROSA)26Sor⁺
involves: 129S6/SvEvTac * C57BL/6 * Swiss Webster

Additional - Gt(ROSA)26Sor^{tm1.2(CAG-EGFP)Fsh} related