A C57BL/6J congenic version of this strain is available as Stock No. 028020.
Mice harboring the Adipoq-Cre BAC transgene express Cre recombinase under control of the mouse adiponectin (Adipoq) promoter/enhancer regions within the BAC transgene. Adipoq-Cre BAC transgenic mice are a Cre-lox tool for deletion of floxed sequences in white adipose tissue and brown adipose tissue, but not in activated or resident macrophages.
Evan D Rosen, Beth Israel Deaconess Medical Center and Harvard Medical School
Genetic Background | Generation |
---|---|
|
Allele Type |
---|
Transgenic (Recombinase-expressing) |
Mice hemizygous for this Adipoq-Cre BAC transgene are viable and fertile, with expression of a Cre recombinase directed to adipose tissue by the promoter/regulatory regions of the mouse adiponectin (Adipoq) locus on the BAC transgene. Transcription/translation from the BAC Adipoq locus is disabled, and Cre recombinase expression levels are similar to that of endogenous Adipoq expression. These mice express Cre recombinase effectively in white adipose tissue (WAT) and brown adipose tissue (BAT), but not in macrophages (including adipose-tissue resident macrophages, alveolar macrophages, or thioglycollate-stimulated peritoneal macrophages). The donating investigator reports highly efficient Cre recombinase activity, with no ectopic expression. The phenotype of homozygous mice was not determined by the donating investigator. These Adipoq-Cre BAC transgenic mice may be useful in generating conditional mutations for studying adipose tissue function and storage, obesity, and other metabolic diseases.
To generate the Adipoq-Cre BAC transgenic mice, the 245 kb RP23-90G21 bacterial artificial chromosome (BAC) containing at least five mouse genes was modified by replacing the starting ATG and 222 bp of the adiponectin gene with the starting ATG and coding sequence of a Cre recombinase gene. This modified BAC transgene was microinjected into pronuclei of fertilized one-cell stage FVB/NJ embryos. Transgenic mice were identified and bred to C57BL/6J wildtype mice to establish founder lines. A single transgenic founder line was characterized. The donating investigator reported that transgenic mice were then backcrossed to C57BL/6J wildtype mice for more than 14 generations prior to sending to The Jackson Laboratory Repository (see 2011 SNP notes below). Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) to establish our live colony. By 2014, the colony was additionally backcrossed to C57BL/6J inbred mice for at least five generations and confirmed by SNP analysis (below) to be C57BL/6J congenic.
In 2011, a 32 SNP (single nucleotide polymorphism) panel analysis, with markers covering all 19 chromosomes and the X chromosome, was performed on the rederived living colony at The Jackson Laboratory Repository. This revealed six markers representing six different chromosomes that were not fixed as C57BL/6 allele-type. Five of these markers, one each on chromosomes 2, 8, 9, 15, and 19, are segregating for C57BL/6 or FVB allele-type. The marker at ~88.2 Mbp on chromosome 16 (that is not polymorphic between C57BL/6 and FVB) was found to be heterozygous for C57BL/6 and an undefined allele-type (i.e., not FVB) in all first generation rederived mice tested. Collectively, these data suggest the mice may not have been backcrossed to C57BL/6 for as many generations as originally reported prior to arrival at The Jackson Laboratory Repository. In addition, the chromosome 16 marker represents an unknown source of genetic contamination.
By 2014, the same 32 SNP panel assay was performed on a cohort of hemizygous females (from several litters) from our colony after five generations of backcrossing onto C57BL/6J. This revealed all markers but one were C57BL/6 allele-type. The single heterozygous (C57BL/6J;FVB/NJ) marker is on chromosome 9 (~53 cM). These data suggest the colony is now C57BL/6J congenic, and the transgene insertion site(s) may be on chromosome 9.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
---|---|
Site of Expression | Mice express Cre recombinase effectively in white adipose tissue (WAT) and brown adipose tissue (BAT), but not in macrophages (including adipose-tissue resident macrophages, alveolar macrophages, or thioglycollate-stimulated peritoneal macrophages). |
Allele Name | transgene insertion 1, Evan Rosen |
---|---|
Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | A-Cre; Adipoq-Cre; Adn-Cre; Tg(Adipoq-CreERT2)tm1Jgg |
Gene Symbol and Name | Tg(Adipoq-cre)1Evdr, transgene insertion 1, Evan Rosen |
Gene Synonym(s) | |
Promoter | Adipoq, adiponectin, C1Q and collagen domain containing, mouse, laboratory |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | Mice express Cre recombinase effectively in white adipose tissue (WAT) and brown adipose tissue (BAT), but not in macrophages (including adipose-tissue resident macrophages, alveolar macrophages, or thioglycollate-stimulated peritoneal macrophages). |
Strain of Origin | FVB/NJ |
Chromosome | UN |
Molecular Note | To generate the Adipoq-Cre BAC transgenic mice, the 245 kb RP23-90G21 bacterial artificial chromosome (BAC) containing at least five mouse genes was modified by replacing the starting ATG and 222 bp of the adiponectin gene with the starting ATG and coding sequence of a Cre recombinase gene. Founder line 1 was analyzed. These mice express Cre recombinase effectively in white adipose tissue (WAT) and brown adipose tissue (BAT), but not in macrophages (including adipose-tissue resident macrophages, alveolar macrophages, or thioglycollate-stimulated peritoneal macrophages). The donating investigator reports highly efficient Cre recombinase activity, with no ectopic expression. |
Mutations Made By | Evan Rosen, Beth Israel Deaconess Medical Center and Harvard Medical School |
When maintaining a live colony, transgene carrier mice may be bred to wildtype (noncarrier) mice from the colony or to C57BL/6J inbred mice (Stock No. 000664). The donating investigator has not attempted to generate homozygous mice.
When using the Adipoq-Cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #010803 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous or non carrier for Tg(Adipoq-cre)1Evdr |
Frozen Mouse Embryo | B6;FVB-Tg(Adipoq-cre)1Evdr/J | $2595.00 |
Frozen Mouse Embryo | B6;FVB-Tg(Adipoq-cre)1Evdr/J | $2595.00 |
Frozen Mouse Embryo | B6;FVB-Tg(Adipoq-cre)1Evdr/J | $3373.50 |
Frozen Mouse Embryo | B6;FVB-Tg(Adipoq-cre)1Evdr/J | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.