A C57BL/6J congenic version of this strain is available as Stock No. 028020.
Mice harboring the Adipoq-Cre BAC transgene express Cre recombinase under control of the mouse adiponectin (Adipoq) promoter/enhancer regions within the BAC transgene. Adipoq-Cre BAC transgenic mice are a Cre-lox tool for deletion of floxed sequences in white adipose tissue and brown adipose tissue, but not in activated or resident macrophages.
Evan D Rosen, Beth Israel Deaconess Medical Center and Harvard Medical School
Genetic Background | Generation |
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Allele Type |
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Transgenic (Recombinase-expressing) |
Mice hemizygous for this Adipoq-Cre BAC transgene are viable and fertile, with expression of a Cre recombinase directed to adipose tissue by the promoter/regulatory regions of the mouse adiponectin (Adipoq) locus on the BAC transgene. Transcription/translation from the BAC Adipoq locus is disabled, and Cre recombinase expression levels are similar to that of endogenous Adipoq expression. These mice express Cre recombinase effectively in white adipose tissue (WAT) and brown adipose tissue (BAT), but not in macrophages (including adipose-tissue resident macrophages, alveolar macrophages, or thioglycollate-stimulated peritoneal macrophages). The donating investigator reports highly efficient Cre recombinase activity, with no ectopic expression. The phenotype of homozygous mice was not determined by the donating investigator. These Adipoq-Cre BAC transgenic mice may be useful in generating conditional mutations for studying adipose tissue function and storage, obesity, and other metabolic diseases.
To generate the Adipoq-Cre BAC transgenic mice, the 245 kb RP23-90G21 bacterial artificial chromosome (BAC) containing at least five mouse genes was modified by replacing the starting ATG and 222 bp of the adiponectin gene with the starting ATG and coding sequence of a Cre recombinase gene. This modified BAC transgene was microinjected into pronuclei of fertilized one-cell stage FVB/NJ embryos. Transgenic mice were identified and bred to C57BL/6J wildtype mice to establish founder lines. A single transgenic founder line was characterized. The donating investigator reported that transgenic mice were then backcrossed to C57BL/6J wildtype mice for more than 14 generations prior to sending to The Jackson Laboratory Repository (see 2011 SNP notes below). Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) to establish our live colony. By 2014, the colony was additionally backcrossed to C57BL/6J inbred mice for at least five generations and confirmed by SNP analysis (below) to be C57BL/6J congenic.
In 2011, a 32 SNP (single nucleotide polymorphism) panel analysis, with markers covering all 19 chromosomes and the X chromosome, was performed on the rederived living colony at The Jackson Laboratory Repository. This revealed six markers representing six different chromosomes that were not fixed as C57BL/6 allele-type. Five of these markers, one each on chromosomes 2, 8, 9, 15, and 19, are segregating for C57BL/6 or FVB allele-type. The marker at ~88.2 Mbp on chromosome 16 (that is not polymorphic between C57BL/6 and FVB) was found to be heterozygous for C57BL/6 and an undefined allele-type (i.e., not FVB) in all first generation rederived mice tested. Collectively, these data suggest the mice may not have been backcrossed to C57BL/6 for as many generations as originally reported prior to arrival at The Jackson Laboratory Repository. In addition, the chromosome 16 marker represents an unknown source of genetic contamination.
By 2014, the same 32 SNP panel assay was performed on a cohort of hemizygous females (from several litters) from our colony after five generations of backcrossing onto C57BL/6J. This revealed all markers but one were C57BL/6 allele-type. The single heterozygous (C57BL/6J;FVB/NJ) marker is on chromosome 9 (~53 cM). These data suggest the colony is now C57BL/6J congenic, and the transgene insertion site(s) may be on chromosome 9.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | Mice express Cre recombinase effectively in white adipose tissue (WAT) and brown adipose tissue (BAT), but not in macrophages (including adipose-tissue resident macrophages, alveolar macrophages, or thioglycollate-stimulated peritoneal macrophages). |
Allele Name | transgene insertion 1, Evan Rosen |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | transgene insertion 1, Evan Rosen; Tg(Adipoq-cre)1Evdr |
Gene Symbol and Name | Tg(Adipoq-cre)1Evdr, transgene insertion 1, Evan Rosen |
Gene Synonym(s) | Adipoq-Cre |
Promoter | Adipoq, adiponectin, C1Q and collagen domain containing, mouse, laboratory |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | Mice express Cre recombinase effectively in white adipose tissue (WAT) and brown adipose tissue (BAT), but not in macrophages (including adipose-tissue resident macrophages, alveolar macrophages, or thioglycollate-stimulated peritoneal macrophages). |
Strain of Origin | FVB/NJ |
Chromosome | UN |
Molecular Note | To generate the Adipoq-Cre BAC transgenic mice, the 245 kb RP23-90G21 bacterial artificial chromosome (BAC) containing at least five mouse genes was modified by replacing the starting ATG and 222 bp of the adiponectin gene with the starting ATG and coding sequence of a Cre recombinase gene. Founder line 1 was analyzed. These mice express Cre recombinase effectively in white adipose tissue (WAT) and brown adipose tissue (BAT), but not in macrophages (including adipose-tissue resident macrophages, alveolar macrophages, or thioglycollate-stimulated peritoneal macrophages). The donating investigator reports highly efficient Cre recombinase activity, with no ectopic expression. |
Mutations Made By | Evan Rosen, Beth Israel Deaconess Medical Center and Harvard Medical School |
When maintaining a live colony, transgene carrier mice may be bred to wildtype (noncarrier) mice from the colony or to C57BL/6J inbred mice (Stock No. 000664). The donating investigator has not attempted to generate homozygous mice.
When using the Adipoq-Cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #010803 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service | Genotype | Price |
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Hemizygous or non carrier for Tg(Adipoq-cre)1Evdr |
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
Frozen Mouse Embryo | B6;FVB-Tg(Adipoq-cre)1Evdr/J | $2595.00 |
Frozen Mouse Embryo | B6;FVB-Tg(Adipoq-cre)1Evdr/J | $2595.00 |
Frozen Mouse Embryo | B6;FVB-Tg(Adipoq-cre)1Evdr/J | $3373.50 |
Frozen Mouse Embryo | B6;FVB-Tg(Adipoq-cre)1Evdr/J | $3373.50 |
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