Overview

Also Known As: Pv-CreER

The Pv-CreERT2 knock-in allele in these mice both abolishes parvalbumin gene function and expresses the CreERT2 fusion protein directed to parvalbumin positive neurons in neocortex and cerebellum by the endogenous Pvalb promoter/enhancer elements.

Donating Investigator

Z. Josh Huang, Cold Spring Harbor Laboratory

Genetic overview

Genetic Background	Generation

Pvalb^{tm1(cre/ERT2)Zjh}

Allele Type	Gene Symbol	Gene Name
Targeted (Recombinase-expressing, Inducible)	Pvalb	parvalbumin

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Research Applications

Research Tools
Neurobiology Research

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

The Pv-CreERT2 (Pvalb-CreERT2) knockin/knockout allele both abolishes parvalbumin (Pvalb; also called PV or Pva) gene function and expresses a CreER^T2 fusion protein (creERT2 fusion protein) from the Pvalb promoter/enhancer elements. Homozygous mice are viable and fertile. Homozygotes are expected to have a knockout phenotype similar to other null mutations of this gene and may exhibit muscle contractile, mitochondrial, and/or Purkinje cell abnormalities. Cre-ERT2 fusion gene activity is inducible; observed following tamoxifen administration. As such, when Pv-CreERT2 mice are bred with mice containing loxP-flanked sequence(s), tamoxifen-inducible Cre-mediated recombination will result in deletion of the floxed sequence(s) in the Pvalb-expressing cells of the offspring.

The donating investigator reports tamoxifen-inducible Cre recombinase activity recapitulates the endogenous Pvalb expression pattern, but with low efficiency of induction. They report tamoxifen-inducible Cre recombinase activity is observed in parvalbumin positive neurons in neocortex and cerebellum, and did not examine cre expression in tissues other than brain.

For characterization information, see images at the Allen Institute for Brain Science website (Pvalb-CreERT2 images).

The Cre-ERT2 fusion protein consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OHT or tamoxifen) and, with lesser sensitivity, ICI 182780. Restricted to the cytoplasm, Cre-ERT2 can only gain access to the nuclear compartment after exposure to tamoxifen. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone may be coadministered.

Development

A targeting vector was designed to insert a CreER^T2 fusion gene (Cre-ER^T2; Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain), an SV40 polyA signal, and an frt-flanked neo cassette into the initiation codon of the Pvalb (parvalbumin; also called PV or Pva) gene. This construct was electroporated into C57BL/6-derived Bruce4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with white C57BL/6 mice to originate the colony. Mutant mice were bred with Actin-FLPe mice (on a C57BL/6 congenic background (N10); see Stock No. 005703) to remove the neo selection cassette. Pv-CreERT2 mice were subsequently bred together for many generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred with C57BL/6J inbred mice (Stock No. 000664) for at least one generation (and the FLPe transgene was selectively bred away) to establish the Pv-CreERT2 colony.

SNP (single nucleotide polymorphism) analysis performed by The Jackson Laboratory revealed that the Donating Investigator used C57BL/6N (2 of 5 markers segregating) in the development of the strain.

Expression Data

Expressed Gene	cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene,
Site of Expression	tamoxifen-inducible Cre recombinase activity is observed in parvalbumin positive neurons in neocortex and cerebellum.

Control Suggestions

Wild-type from the colony
000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

NIH Neuroscience Blueprint Cre Driver Network. 2009. Cre recombinase-expressing mice generated for the NIH Neuroscience Blueprint Cre Driver Network MGI Direct Data SubmissionMGI: J:151755
Taniguchi H; He M; Wu P; Kim S; Paik R; Sugino K; Kvitsani D; Fu Y; Lu J; Lin Y; Miyoshi G; Shima Y; Fishell G; Nelson SB; Huang ZJ. 2011. A resource of cre driver lines for genetic targeting of GABAergic neurons in cerebral cortex. Neuron 71(6):995-1013PubMed: 21943598 MGI: J:177261

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Genetics

Pvalb^{tm1(cre/ERT2)Zjh}

Allele Symbol: Pvalb^{tm1(cre/ERT2)Zjh}

Allele Name	targeted mutation 1, Z Josh Huang
Allele Type	Targeted (Recombinase-expressing, Inducible)
Allele Synonym(s)	Pvalb^{tm1(cre/ERT2)Zjh}; targeted mutation 1, Z Josh Huang
Gene Symbol and Name	Pvalb, parvalbumin
Gene Synonym(s)	Pva; PV; Pva; Parv; D22S749; PALB1
Expressed Gene	cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene,
Site of Expression	tamoxifen-inducible Cre recombinase activity is observed in parvalbumin positive neurons in neocortex and cerebellum.
Strain of Origin	C57BL/6
Chromosome	15
Molecular Note	A targeting vector was designed to insert a CreERT2 fusion gene (Cre-ERT2; Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain), an SV40 polyA signal, and an frt-flanked neo cassette into the initiation codon of the Pvalb (parvalbumin; also called PV or Pva) gene. Mutant mice were bred with Actin-FLPe mice (on a C57BL/6 congenic background) to remove the neo selection cassette.
Mutations Made By	Z. Josh Huang, Cold Spring Harbor Laboratory

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Research Tools
- Genetics Research
  - Tissue/Cell Markers: neurons
  - Tissue/Cell Markers: Cre-lox System
  - Mutagenesis and Transgenesis
  - Tissue/Cell Markers
  - Mutagenesis and Transgenesis: Cre-lox System
- Neurobiology Research
  - cell marker
- Cre-lox System
  - Cre Recombinase Expression
  - Cre Recombinase Expression: Inducible
Neurobiology Research
- Cre-lox System
  - Cre Recombinase expression in neural tissue

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Pvalb^{tm1(cre/ERT2)Zjh}/Pvalb⁺
involves: C57BL/6

normal phenotype

no abnormal phenotype detected
- Normal - mice are viable, fertile, normal in size and display no overt physical or behavioral abnormalities

References

NIH Neuroscience Blueprint Cre Driver Network. 2009. Cre recombinase-expressing mice generated for the NIH Neuroscience Blueprint Cre Driver Network MGI Direct Data SubmissionMGI: J:151755
Taniguchi H; He M; Wu P; Kim S; Paik R; Sugino K; Kvitsani D; Fu Y; Lu J; Lin Y; Miyoshi G; Shima Y; Fishell G; Nelson SB; Huang ZJ. 2011. A resource of cre driver lines for genetic targeting of GABAergic neurons in cerebral cortex. Neuron 71(6):995-1013PubMed: 21943598 MGI: J:177261

Additional - Pvalb^{tm1(cre/ERT2)Zjh} related

Technical Support

Contact Technical Support

Genotyping Protocols
Breeding Considerations

When maintaining a live colony, heterozygous mice may be bred together, to wildtype siblings, or to C57BL/6J mice (Stock No. 000664). The donating investigator reports that they breed homozygous mice together.
- Additional Breeding and Husbandry Support
Citation
When using the Pv-CreER mouse strain in a publication, please cite the originating article(s) and include JAX stock #010777 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous or wildtype for Pvalb<tm1(cre/ERT2)Zjh>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

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Also Known As: Pv-CreER

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Pvalbtm1(cre/ERT2)Zjh

Allele Symbol: Pvalbtm1(cre/ERT2)Zjh

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Pvalbtm1(cre/ERT2)Zjh/Pvalb+involves: C57BL/6

normal phenotype

References

Additional - Pvalbtm1(cre/ERT2)Zjh related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Pvalb^{tm1(cre/ERT2)Zjh}

Allele Symbol: Pvalb^{tm1(cre/ERT2)Zjh}

Genotype: Pvalb^{tm1(cre/ERT2)Zjh}/Pvalb⁺
involves: C57BL/6

Additional - Pvalb^{tm1(cre/ERT2)Zjh} related