JAX Mouse Strain Datasheet - 010774

B6(Cg)-Calb2^tm1(cre)Zjh/J

Stock No:: 010774 |; Cr-iCre

Repository Live

Overview

Also Known As: Cr-iCre

Cr-IRES-Cre (Calb2-IRES-Cre) mice have Cre recombinase expression directed to calretinin interneurons in the brain and cortex by the endogenous promoter/enhancer elements of the Calb2 locus (calbindin 2; also called calretinin or CR).

Donating Investigator

Z. Josh Huang, Cold Spring Harbor Laboratory

Genetic overview

Genetic Background	Generation
	N11+N3F6 (16-FEB-18)

Calb2^tm1(cre)Zjh

Allele Type	Gene Symbol	Gene Name
Targeted (Recombinase-expressing)	Calb2	calbindin 2

View Genetics

Research Applications

Neurobiology Research
Research Tools

View All Research Applications

Base Price

Starting at:

$255.00 Domestic price for female

$329.00 Domestic price for breeder pair

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Details

Detailed Description

The Cr-IRES-Cre (Calb2-IRES-Cre) allele harbors an internal ribosome entry site and Cre recombinase in the 3' UTR of the Calb2 locus (calbindin 2; also called calretinin or CR). As such, it is designed to have the endogenous Calb2 promoter/enhancer elements directing cre expression to calretinin interneurons in the brain and cortex. When Calb2-IRES-Cre mice are bred with mice containing loxP-flanked sequences, Cre-mediated recombination will result in deletion of the floxed sequences in the Calb2-expressing cells of the offspring. Calb2 expression from the Calb2-IRES-Cre allele has not been evaluated. Additional phenotype information described below.

In 2009, the donating investigator reported that Cre recombinase activity is specific and efficient (largely recapitulates the endogenous Calb2 expression pattern with highly efficient recombination). They did not examine cre expression in tissues other than brain. Calb2 expression from the Calb2-IRES-Cre allele was not evaluated. They also reported that homozygous mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.

For characterization information, see images at the Allen Institute for Brain Science website (Calb2-IRES-Cre images).

If the recombinase activity pattern of this allele is further characterized by the Genetic Resource Science group at The Jackson Laboratory, such findings will be reported on the Mouse Genome Informatics (MGI) Allele Detail entry (Calb2^tm1(cre)Zjh). This same information would also be found searching the MGI Recombinase Activity database.

Development

A targeting vector was designed to insert an internal ribosome entry site (IRES), a Cre recombinase sequence, an SV40 polyA signal, and an frt-flanked neo cassette into the 3' untranslated region (~60 bp after the translational termination site) of the Calb2 gene (calbindin 2; also called calretinin or CR). This construct was electroporated into C57BL/6-derived Bruce4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with white C57BL/6 mice to originate the colony. Mutant mice were bred with Actin-FLPe mice (on a C57BL/6 congenic background (N10); see Stock No. 005703) to remove the neo selection cassette. These Cr-IRES-Cre (Calb2-IRES-Cre) mice were subsequently bred with wildtype C57BL/6 (see SNP notes below) mice for approximately three generations (and the FLPe transgene was removed) prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred with C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the Cr-IRES-Cre colony.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 4 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.

Expression Data

Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	cre expression is directed to calretinin interneurons in the brain and cortex by the endogenous Calb2 promoter/enhancer elements.

Control Suggestions

Wild-type from the colony
000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

NIH Neuroscience Blueprint Cre Driver Network. 2009. Cre recombinase-expressing mice generated for the NIH Neuroscience Blueprint Cre Driver Network MGI Direct Data Submission :. MGI: J:151755
Taniguchi H; He M; Wu P; Kim S; Paik R; Sugino K; Kvitsani D; Fu Y; Lu J; Lin Y; Miyoshi G; Shima Y; Fishell G; Nelson SB; Huang ZJ. 2011. A resource of cre driver lines for genetic targeting of GABAergic neurons in cerebral cortex. Neuron 71(6):995-1013. PubMed: 21943598 MGI: J:177261

View All References

Genetics

Calb2^tm1(cre)Zjh

Allele Symbol: Calb2^tm1(cre)Zjh

Allele Name	targeted mutation 1, Z Josh Huang
Allele Type	Targeted (Recombinase-expressing)
Allele Synonym(s)	Cr-IRES-cre; Cr-iCre
Gene Symbol and Name	Calb2, calbindin 2
Gene Synonym(s)	CAB29; CAL2; CR; calretinin
Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	cre expression is directed to calretinin interneurons in the brain and cortex by the endogenous Calb2 promoter/enhancer elements.
Strain of Origin	C57BL/6
Chromosome	8
Molecular Note	A targeting vector was designed to insert an internal ribosome entry site (IRES), a Cre recombinase sequence, an SV40 polyA signal, and an frt-flanked neo cassette into the 3' untranslated region (~60 bp after the translational termination site) of the Calb2 (calbindin 2; also called calretinin or CR) gene. Mutant mice were bred with Actin-FLPe mice (on a C57BL/6 congenic background) to remove the neo selection cassette and the transgene was subsequently bred out of the background.
Mutations Made By	Z. Josh Huang, Cold Spring Harbor Laboratory

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Neurobiology Research
- Cre-lox System
  - Cre Recombinase expression in neural tissue
Research Tools
- Cre-lox System
  - Cre Recombinase Expression
- Genetics Research
  - Mutagenesis and Transgenesis
  - Mutagenesis and Transgenesis: Cre-lox System
  - Tissue/Cell Markers
  - Tissue/Cell Markers: Cre-lox System
  - Tissue/Cell Markers: neurons
- Neurobiology Research
  - cell marker

Genotype: cre related

Research Tools
- Cre-lox System
- Genetics Research
  - Mutagenesis and Transgenesis
  - Mutagenesis and Transgenesis: Cre-lox System

Mammalian Phenotype Terms by Genotype

Genotype: Calb2^tm1(cre)Zjh/Calb2^tm1(cre)Zjh
involves: C57BL/6

normal phenotype

no abnormal phenotype detected
- mice are viable, fertile, normal in size and display no overt physical or behavioral abnormalities

References

NIH Neuroscience Blueprint Cre Driver Network. 2009. Cre recombinase-expressing mice generated for the NIH Neuroscience Blueprint Cre Driver Network MGI Direct Data Submission :. MGI: J:151755
Taniguchi H; He M; Wu P; Kim S; Paik R; Sugino K; Kvitsani D; Fu Y; Lu J; Lin Y; Miyoshi G; Shima Y; Fishell G; Nelson SB; Huang ZJ. 2011. A resource of cre driver lines for genetic targeting of GABAergic neurons in cerebral cortex. Neuron 71(6):995-1013. PubMed: 21943598 MGI: J:177261

Additional - Calb2^tm1(cre)Zjh related

Allen Institute for Brain Science. 2009. Cre transgenes and knockin alleles created at the Allen Insitute for Brain Science (AIBS) MGI Direct Data Submission :. MGI: J:146821
Duan B; Cheng L; Bourane S; Britz O; Padilla C; Garcia-Campmany L; Krashes M; Knowlton W; Velasquez T; Ren X; Ross SE; Lowell BB; Wang Y; Goulding M; Ma Q. 2014. Identification of spinal circuits transmitting and gating mechanical pain. Cell 159(6):1417-32. PubMed: 25467445 MGI: J:219216
Genetic Resource Science at The Jackson Laboratory. 2012. Expression/Specificity Patterns of Cre Alleles, 2012 Direct Data Submission from Genetic Resource Science :. MGI: J:184579
Harris JA; Hirokawa KE; Sorensen SA; Gu H; Mills M; Ng LL; Bohn P; Mortrud M; Ouellette B; Kidney J; Smith KA; Dang C; Sunkin S; Bernard A; Oh SW; Madisen L; Zeng H. 2014. Anatomical characterization of Cre driver mice for neural circuit mapping and manipulation. Front Neural Circuits 8:76. PubMed: 25071457 MGI: J:220523
He M; Tucciarone J; Lee S; Nigro MJ; Kim Y; Levine JM; Kelly SM; Krugikov I; Wu P; Chen Y; Gong L; Hou Y; Osten P; Rudy B; Huang ZJ. 2016. Strategies and Tools for Combinatorial Targeting of GABAergic Neurons in Mouse Cerebral Cortex. Neuron :. PubMed: 27618674 MGI: J:235123
Hua ZL; Jeon S; Caterina MJ; Nathans J. 2014. Frizzled3 is required for the development of multiple axon tracts in the mouse central nervous system. Proc Natl Acad Sci U S A 111(29):E3005-14. PubMed: 24799694 MGI: J:212111
Kim Y; Yang GR; Pradhan K; Venkataraju KU; Bota M; Garcia Del Molino LC; Fitzgerald G; Ram K; He M; Levine JM; Mitra P; Huang ZJ; Wang XJ; Osten P. 2017. Brain-wide Maps Reveal Stereotyped Cell-Type-Based Cortical Architecture and Subcortical Sexual Dimorphism. Cell 171(2):456-469.e22. PubMed: 28985566 MGI: J:250011
Leinweber M; Ward DR; Sobczak JM; Attinger A; Keller GB. 2017. A Sensorimotor Circuit in Mouse Cortex for Visual Flow Predictions. Neuron 95(6):1420-1432.e5. PubMed: 28910624 MGI: J:253236
Martersteck EM; Hirokawa KE; Evarts M; Bernard A; Duan X; Li Y; Ng L; Oh SW; Ouellette B; Royall JJ; Stoecklin M; Wang Q; Zeng H; Sanes JR; Harris JA. 2017. Diverse Central Projection Patterns of Retinal Ganglion Cells. Cell Rep 18(8):2058-2072. PubMed: 28228269 MGI: J:247473
Monavarfeshani A; Knill CN; Sabbagh U; Su J; Fox MA. 2017. Region- and Cell-Specific Expression of Transmembrane Collagens in Mouse Brain. Front Integr Neurosci 11(None):20. PubMed: 28912695 MGI: J:247517
Nguyen AQ; Dela Cruz JA; Sun Y; Holmes TC; Xu X. 2016. Genetic cell targeting uncovers specific neuronal types and distinct subregions in the bed nucleus of the stria terminalis. J Comp Neurol 524(12):2379-99. PubMed: 26718312 MGI: J:233612
Nigro MJ; Hashikawa-Yamasaki Y; Rudy B. 2018. Diversity and Connectivity of Layer 5 Somatostatin-Expressing Interneurons in the Mouse Barrel Cortex. J Neurosci 38(7):1622-1633. PubMed: 29326172 MGI: J:257171
Peirs C; Williams SP; Zhao X; Walsh CE; Gedeon JY; Cagle NE; Goldring AC; Hioki H; Liu Z; Marell PS; Seal RP. 2015. Dorsal Horn Circuits for Persistent Mechanical Pain. Neuron 87(4):797-812. PubMed: 26291162 MGI: J:227670
Sweeney NT; Tierney H; Feldheim DA. 2014. Tbr2 is required to generate a neural circuit mediating the pupillary light reflex. J Neurosci 34(16):5447-53. PubMed: 24741035 MGI: J:210625
Tasic B; Menon V; Nguyen TN; Kim TK; Jarsky T; Yao Z; Levi B; Gray LT; Sorensen SA; Dolbeare T; Bertagnolli D; Goldy J; Shapovalova N; Parry S; Lee C; Smith K; Bernard A; Madisen L; Sunkin SM; Hawrylycz M; Koch C; Zeng H. 2016. Adult mouse cortical cell taxonomy revealed by single cell transcriptomics. Nat Neurosci :. PubMed: 26727548 MGI: J:227758
Tyan L; Chamberland S; Magnin E; Camire O; Francavilla R; David LS; Deisseroth K; Topolnik L. 2014. Dendritic inhibition provided by interneuron-specific cells controls the firing rate and timing of the hippocampal feedback inhibitory circuitry. J Neurosci 34(13):4534-47. PubMed: 24671999 MGI: J:210454
Vasquez-Lopez SA; Weissenberger Y; Lohse M; Keating P; King AJ; Dahmen JC. 2017. Thalamic input to auditory cortex is locally heterogeneous but globally tonotopic. Elife 6(None):None. PubMed: 28891466 MGI: J:245083
Zhou N; Masterson SP; Damron JK; Guido W; Bickford ME. 2018. The Mouse Pulvinar Nucleus Links the Lateral Extrastriate Cortex, Striatum, and Amygdala. J Neurosci 38(2):347-362. PubMed: 29175956 MGI: J:257360
Zhu Y; Xu J; Hauswirth WW; DeVries SH. 2014. Genetically targeted binary labeling of retinal neurons. J Neurosci 34(23):7845-61. PubMed: 24899708 MGI: J:211630

Pricing & Availability

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Age

Genotype

Price

weeks

Breeder Pair

Sex

Genotype

Price

Female
Male

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous or wildtype for Calb2<tm1(cre)Zjh>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

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Also Known As: Cr-iCre

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Calb2tm1(cre)Zjh

Allele Symbol: Calb2tm1(cre)Zjh

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Genotype: cre related

Mammalian Phenotype Terms by Genotype

Genotype: Calb2tm1(cre)Zjh/Calb2tm1(cre)Zjhinvolves: C57BL/6

normal phenotype

References

Additional - Calb2tm1(cre)Zjh related

Genotyping Protocols

Dietary Information

Breeding Considerations

Mating System

Citation

Animal Health Reports

Live Mouse

Breeder Pair

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Calb2^tm1(cre)Zjh

Allele Symbol: Calb2^tm1(cre)Zjh

Genotype: Calb2^tm1(cre)Zjh/Calb2^tm1(cre)Zjh
involves: C57BL/6

Additional - Calb2^tm1(cre)Zjh related