Overview

Also Known As: Cr-iCre

Cr-IRES-Cre (Calb2-IRES-Cre) mice have Cre recombinase expression directed to calretinin interneurons in the brain and cortex by the endogenous promoter/enhancer elements of the Calb2 locus (calbindin 2; also called calretinin or CR).

Donating Investigator

Z. Josh Huang, Cold Spring Harbor Laboratory

Genetic overview

Genetic Background	Generation
	N11+N3F9 (2019-03-14 00:00:00)

Calb2^tm1(cre)Zjh

Allele Type	Gene Symbol	Gene Name
Targeted (Recombinase-expressing)	Calb2	calbindin 2

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Research Applications

Research Tools
Neurobiology Research

View All Research Applications

Base Price

Starting at:

$255.00 Domestic price for female 4-week

333.51 Domestic price for breeder pair

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Details

Detailed Description

The Cr-IRES-Cre (Calb2-IRES-Cre) allele harbors an internal ribosome entry site and Cre recombinase in the 3' UTR of the Calb2 locus (calbindin 2; also called calretinin or CR). As such, it is designed to have the endogenous Calb2 promoter/enhancer elements directing cre expression to calretinin interneurons in the brain and cortex. When Calb2-IRES-Cre mice are bred with mice containing loxP-flanked sequences, Cre-mediated recombination will result in deletion of the floxed sequences in the Calb2-expressing cells of the offspring. Calb2 expression from the Calb2-IRES-Cre allele has not been evaluated. Additional phenotype information described below.

In 2009, the donating investigator reported that Cre recombinase activity is specific and efficient (largely recapitulates the endogenous Calb2 expression pattern with highly efficient recombination). They did not examine cre expression in tissues other than brain. Calb2 expression from the Calb2-IRES-Cre allele was not evaluated. They also reported that homozygous mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.

For characterization information, see images at the Allen Institute for Brain Science website (Calb2-IRES-Cre images).

If the recombinase activity pattern of this allele is further characterized by the Genetic Resource Science group at The Jackson Laboratory, such findings will be reported on the Mouse Genome Informatics (MGI) Allele Detail entry (Calb2^tm1(cre)Zjh). This same information would also be found searching the MGI Recombinase Activity database.

Development

A targeting vector was designed to insert an internal ribosome entry site (IRES), a Cre recombinase sequence, an SV40 polyA signal, and an frt-flanked neo cassette into the 3' untranslated region (~60 bp after the translational termination site) of the Calb2 gene (calbindin 2; also called calretinin or CR). This construct was electroporated into C57BL/6-derived Bruce4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with white C57BL/6 mice to originate the colony. Mutant mice were bred with Actin-FLPe mice (on a C57BL/6 congenic background (N10); see Stock No. 005703) to remove the neo selection cassette. These Cr-IRES-Cre (Calb2-IRES-Cre) mice were subsequently bred with wildtype C57BL/6 (see SNP notes below) mice for approximately three generations (and the FLPe transgene was removed) prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred with C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the Cr-IRES-Cre colony.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 4 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.

Expression Data

Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	cre expression is directed to calretinin interneurons in the brain and cortex by the endogenous Calb2 promoter/enhancer elements.

Control Suggestions

Wild-type from the colony
000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

NIH Neuroscience Blueprint Cre Driver Network. 2009. Cre recombinase-expressing mice generated for the NIH Neuroscience Blueprint Cre Driver Network MGI Direct Data SubmissionMGI: J:151755
Taniguchi H; He M; Wu P; Kim S; Paik R; Sugino K; Kvitsani D; Fu Y; Lu J; Lin Y; Miyoshi G; Shima Y; Fishell G; Nelson SB; Huang ZJ. 2011. A resource of cre driver lines for genetic targeting of GABAergic neurons in cerebral cortex. Neuron 71(6):995-1013PubMed: 21943598 MGI: J:177261

View All References

Genetics

Calb2^tm1(cre)Zjh

Allele Symbol: Calb2^tm1(cre)Zjh

Allele Name	targeted mutation 1, Z Josh Huang
Allele Type	Targeted (Recombinase-expressing)
Allele Synonym(s)	targeted mutation 1, Z Josh Huang; Calb2^tm1(cre)Zjh
Gene Symbol and Name	Calb2, calbindin 2
Gene Synonym(s)	CAB29; CAL2; CR; calretinin
Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	cre expression is directed to calretinin interneurons in the brain and cortex by the endogenous Calb2 promoter/enhancer elements.
Strain of Origin	C57BL/6
Chromosome	8
Molecular Note	A targeting vector was designed to insert an internal ribosome entry site (IRES), a Cre recombinase sequence, an SV40 polyA signal, and an frt-flanked neo cassette into the 3' untranslated region (~60 bp after the translational termination site) of the Calb2 (calbindin 2; also called calretinin or CR) gene. Mutant mice were bred with Actin-FLPe mice (on a C57BL/6 congenic background) to remove the neo selection cassette and the transgene was subsequently bred out of the background.
Mutations Made By	Z. Josh Huang, Cold Spring Harbor Laboratory

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Research Tools
- Genetics Research
  - Tissue/Cell Markers: neurons
  - Tissue/Cell Markers: Cre-lox System
  - Mutagenesis and Transgenesis
  - Tissue/Cell Markers
  - Mutagenesis and Transgenesis: Cre-lox System
- Neurobiology Research
  - cell marker
- Cre-lox System
  - Cre Recombinase Expression
Neurobiology Research
- Cre-lox System
  - Cre Recombinase expression in neural tissue

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Calb2^tm1(cre)Zjh/Calb2^tm1(cre)Zjh
involves: C57BL/6

normal phenotype

no abnormal phenotype detected
- Normal - mice are viable, fertile, normal in size and display no overt physical or behavioral abnormalities

References

NIH Neuroscience Blueprint Cre Driver Network. 2009. Cre recombinase-expressing mice generated for the NIH Neuroscience Blueprint Cre Driver Network MGI Direct Data SubmissionMGI: J:151755
Taniguchi H; He M; Wu P; Kim S; Paik R; Sugino K; Kvitsani D; Fu Y; Lu J; Lin Y; Miyoshi G; Shima Y; Fishell G; Nelson SB; Huang ZJ. 2011. A resource of cre driver lines for genetic targeting of GABAergic neurons in cerebral cortex. Neuron 71(6):995-1013PubMed: 21943598 MGI: J:177261

Additional - Calb2^tm1(cre)Zjh related

Technical Support

Contact Technical Support

Genotyping Protocols
- Standard PCR:Calb2^tm1(cre)Zjh
- Standard PCR:Calb2^tm1(cre)Zjh
- Genotyping resources and troubleshooting
Dietary Information
- LabDiet® 5K52 formulation (6% fat)
Breeding Considerations

When maintaining a live colony, homozygous mice may be bred together.
- Additional Breeding and Husbandry Support
Mating System
- Heterozygote x +/+ sibling
Citation
When using the Cr-iCre mouse strain in a publication, please cite the originating article(s) and include JAX stock #010774 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

AX10 (Standard)

Pricing & Availability

Available

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Breeder Pair

Sex

Genotype

Price

Female
Male

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous or wildtype for Calb2<tm1(cre)Zjh>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Related Products and Services

Frozen Mouse Embryo	B6(Cg)-Calb2<tm1(cre)Zjh>/J	$2595.00
Frozen Mouse Embryo	B6(Cg)-Calb2<tm1(cre)Zjh>/J	$2595.00
Frozen Mouse Embryo	B6(Cg)-Calb2<tm1(cre)Zjh>/J	$3373.50
Frozen Mouse Embryo	B6(Cg)-Calb2<tm1(cre)Zjh>/J	$3373.50

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Also Known As: Cr-iCre

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Calb2tm1(cre)Zjh

Allele Symbol: Calb2tm1(cre)Zjh

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Calb2tm1(cre)Zjh/Calb2tm1(cre)Zjhinvolves: C57BL/6

normal phenotype

References

Additional - Calb2tm1(cre)Zjh related

Genotyping Protocols

Dietary Information

Breeding Considerations

Mating System

Citation

Animal Health Reports

Live Mouse

Breeder Pair

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Calb2^tm1(cre)Zjh

Allele Symbol: Calb2^tm1(cre)Zjh

Genotype: Calb2^tm1(cre)Zjh/Calb2^tm1(cre)Zjh
involves: C57BL/6

Additional - Calb2^tm1(cre)Zjh related