This targeted mutation of the potassium voltage-gated channel, shaker-related subfamily, member 2 (Kcna2) gene exhibits spontaneous seizures may be useful in studies of voltage dependent potassium channels and epilepsy.
Dr. Bruce L. Tempel, Univ of Washington School of Medicine
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Kcna2 | potassium voltage-gated channel, shaker-related subfamily, member 2 |
Homozygous mice appear normal at birth, however, by day 15 mice are smaller and exhibit explosive running and bouncing seizures sometimes followed by tonic extension accompanied by a shortness of breath (apnea). These seizures occur at a rate of less than one per day and have a 50% fatality rate. Mean lifespan is approximately 17 days on both the mixed C57BL/6-129S7 background and the C3H background. In homozygous mice, auditory neurons of the medial nucleus of the trapezoid body (MNTB) exhibit a decrease in action potential firing (hypoexcitable) and higher threshold current amplitude than wild-type mice. MNTB neurons in heterozygous mice exhibit an intermediate phenotype. The counter-intuitive decrease in activity with the deletion of Kv1.2 subunits occurs because replacement subunits (most likely Kv1.1) are lower-threshold than Kv1.2. This mutant mouse strain may be useful in studies of voltage dependent potassium channels and epilepsy.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A targeting vector containing the neomycin resistance gene was used to replace the open reading frame of the targeted gene. The construct was electroporated into 129S7/SvEvTac derived AB1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to C3HeB/FeJ and backcrossed for greater than 30 generations. In 2008, as a result of breeding difficulties, the strain was backcrossed to CBA/CaJ for 2 generations. Upon arrival, mice were bred to C3HeB/FeJ for at least 1 generation to establish the colony.
Allele Name | targeted mutation 1, Bruce L Tempel |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | |
Gene Symbol and Name | Kcna2, potassium voltage-gated channel, shaker-related subfamily, member 2 |
Gene Synonym(s) | |
Strain of Origin | 129S7/SvEvBrd-Hprt+ |
Chromosome | 3 |
Molecular Note | A neomycin resistance cassette replaced the entire open reading frame. mRNAQ cannot be found by quantitative PCR and protein product cannot be identified by Western blot in extracts from homozygous mutant mice. |
Mutations Made By | Dr. Bruce Tempel, Univ of Washington School of Medicine |
While maintaining a live colony, these mice are bred as heteroygotes. Mice homozygous for the mutation die by 17 days of age.
When using the STOCK Kcna2tm1Tem/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #010744 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or wildytpe for Kcna2<tm1Tem> |
Frozen Mouse Embryo | STOCK Kcna2<tm1Tem>/J | $2595.00 |
Frozen Mouse Embryo | STOCK Kcna2<tm1Tem>/J | $2595.00 |
Frozen Mouse Embryo | STOCK Kcna2<tm1Tem>/J | $3373.50 |
Frozen Mouse Embryo | STOCK Kcna2<tm1Tem>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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