| Allele Name | targeted mutation 44, Elizabeth M Simpson |
| Allele Type | Targeted |
| Allele Synonym(s) | DBH-B-lacZ; Hprttm44(mEMS3682)Ems; Ple49 |
| Gene Symbol and Name | Hprt, hypoxanthine guanine phosphoribosyl transferase |
| Gene Synonym(s) | |
| Promoter | DBH, dopamine beta-hydroxylase, human |
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| Expressed Gene | lacZ, beta-galactosidase, E. coli |
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| Site of Expression | lacZ is expressed in noradrenergic cells in the locus coeruleus and in the adrenal gland. |
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| Strain of Origin | (B6.129P2-Hprtb-m3/J x 129S-Gt(ROSA)26Sortm1Sor/J)F1 |
| Chromosome | X |
| General Note | Germ line transmission of mutant cell line mEMS3682 has been established. |
| Molecular Note | The Ple49-lacZ transgene (pEMS1520) was designed with the 756 bp Ple49 minipromoter (DBH-B; derived from a subsection of the promoter and an upstream putative regulatory element of the human dopamine beta-hydroxylase (dopamine beta-monooxygenase) [DBH] gene) upstream of a frt-flanked beta-galactosidase (lacZ) gene, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprtb-m3 mutant locus on the X chromosome. The promoter/regulatory regions of the human dopamine beta-hydroxylase (DBH) gene directs expression of beta galactosidase (lacZ) to the noradrenergic cells in the locus coeruleus, with strong expression in the adrenal gland where it is coexpressed with tyrosine hydroxylase. |
| Mutations Made By | Elizabeth Simpson, Centre for Molecular Medicine & Therapeutics, University of British Columbia |
