B6.129P2(Cg)-Hprt1^{tm44(Ple49-lacZ)Ems}/Mmjax

Stock number: 010709
Strain synonyms: B6.129P2(Cg)-Hprt
Research areas: Neurobiology Research, Research Tools

Description

These mice have the Ple49-lacZ transgene targeted as a single copy "knockin" into the upstream region of the Hprt1 locus on the X chromosome. The promoter/regulatory regions of the human DBH gene direct expression of β-galactosidase.

Allele

Symbol: Hprt1^{tm44(Ple49-lacZ)Ems}
Name: targeted mutation 44, Elizabeth M Simpson
MGI accession ID: MGI:4834779
Generation method: Targeted
Marker symbol: Hprt1
Marker name: hypoxanthine phosphoribosyltransferase 1
Chromosome: X
Strain of origin: (B6.129P2-Hprt1^b-m3/J x 129S-Gt(ROSA)26Sor^tm1Sor/J)F1
Expressed genes: lacZ,beta-galactosidase,E. coli
Site of expression: lacZ is expressed in noradrenergic cells in the locus coeruleus and in the adrenal gland.
Molecular note: The Ple49-lacZ transgene (pEMS1520) was designed with the 756 bp Ple49 minipromoter (DBH-B; derived from a subsection of the promoter and an upstream putative regulatory element of the human dopamine beta-hydroxylase (dopamine beta-monooxygenase) [DBH] gene) upstream of a frt-flanked beta-galactosidase (lacZ) gene, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprt^b-m3 mutant locus on the X chromosome.

The promoter/regulatory regions of the human dopamine beta-hydroxylase (DBH) gene directs expression of beta galactosidase (lacZ) to the noradrenergic cells in the locus coeruleus, with strong expression in the adrenal gland where it is coexpressed with tyrosine hydroxylase.
General note: Germ line transmission of mutant cell line mEMS3682 has been established.