These mice have the Promoterless-lacZ transgene targeted as a single copy "knockin" into the upstream region of the Hprt locus on the X chromosome. As there is no Pleiades minipromoter directing expression of β-galactosidase (lacZ), no lacZ expression pattern is seen or anticipated. These Promoterless-lacZ;mEMS3689 mice may be useful as the primary negative control strain for other Pleiades minipromoter lacZ strains.
Elizabeth M Simpson, Centre for Molecular Medicine & Therapeutics, University of British Columbia
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted | Hprt | hypoxanthine guanine phosphoribosyl transferase |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Site of Expression | This is a promoter-less negative control strain for Pleiades strain expressing lacZ. No lacZ expression is evident. |
Allele Name | targeted mutation 37, Elizabeth M Simpson |
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Allele Type | Targeted |
Allele Synonym(s) | Hprttm37(mEMS3689)Ems; Ptomoterless-lacZ-mEMA3689 |
Gene Symbol and Name | Hprt, hypoxanthine guanine phosphoribosyl transferase |
Gene Synonym(s) | |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | This is a promoter-less negative control strain for Pleiades strain expressing lacZ. No lacZ expression is evident. |
Strain of Origin | (B6.129P2-Hprtb-m3/J x 129S-Gt(ROSA)26Sortm1Sor/J)F1 |
Chromosome | X |
General Note | Germ line transmission of mutant cell line mEMS3689 has been established. |
Molecular Note | The "Promoterless-lacZ" transgene (pEMS1313) was designed with no Pleiades minipromoter upstream of a frt-flanked _-galactosidase (lacZ) gene, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprtb-m3 mutant locus on the X chromosome. Without a promoter directing expression of beta-galactosidase (lacZ), no lacZ expression pattern is seen or anticipated. |
Mutations Made By | Elizabeth Simpson, Centre for Molecular Medicine & Therapeutics, University of British Columbia |
The donating investigator recommends maintaining this strain by breeding heterozygous females with C57BL/6J inbred males.
When using the STOCK Hprttm37(lacZ)Ems/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #32935 in your Materials and Methods section.
Facility Barrier Level Descriptions
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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