STOCK Hprt^{tm37(lacZ)Ems}/Mmjax

Stock number: 010707
Research areas: Neurobiology Research, Research Tools

Description

These mice have the Promoterless-lacZ transgene targeted as a single copy "knockin" into the upstream region of the Hprt locus on the X chromosome. As there is no Pleiades minipromoter directing expression of β-galactosidase (lacZ), no lacZ expression pattern is seen or anticipated. These Promoterless-lacZ;mEMS3689 mice may be useful as the primary negative control strain for other Pleiades minipromoter lacZ strains.

Allele

Symbol: Hprt^{tm37(lacZ)Ems}
Name: targeted mutation 37, Elizabeth M Simpson
MGI accession ID: MGI:4834777
Generation method: Targeted
Synonyms: Hprt^{tm37(mEMS3689)Ems}; Ptomoterless-lacZ-mEMA3689
Marker symbol: Hprt
Marker name: hypoxanthine guanine phosphoribosyl transferase
Chromosome: X
Strain of origin: (B6.129P2-Hprt^b-m3/J x 129S-Gt(ROSA)26Sor^tm1Sor/J)F1
Expressed genes: lacZ,beta-galactosidase,E. coli
Site of expression: This is a promoter-less negative control strain for Pleiades strain expressing lacZ. No lacZ expression is evident.
Molecular note: The "Promoterless-lacZ" transgene (pEMS1313) was designed with no Pleiades minipromoter upstream of a frt-flanked _-galactosidase (lacZ) gene, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprt^b-m3 mutant locus on the X chromosome. Without a promoter directing expression of beta-galactosidase (lacZ), no lacZ expression pattern is seen or anticipated.
General note: Germ line transmission of mutant cell line mEMS3689 has been established.