Overview

These mice have the Promoterless-lacZ transgene targeted as a single copy "knockin" into the upstream region of the Hprt locus on the X chromosome. As there is no Pleiades minipromoter directing expression of β-galactosidase (lacZ), no lacZ expression pattern is seen or anticipated. These Promoterless-lacZ;mEMS3689 mice may be useful as the primary negative control strain for other Pleiades minipromoter lacZ strains.

Donating Investigator

Elizabeth M Simpson, Centre for Molecular Medicine & Therapeutics, University of British Columbia

Genetic overview

Genetic Background	Generation

Hprt^{tm37(lacZ)Ems}

Allele Type	Gene Symbol	Gene Name
Targeted	Hprt	hypoxanthine guanine phosphoribosyl transferase

View Genetics

Research Applications

Neurobiology Research
Research Tools

View All Research Applications

Details

Expression Data

Expressed Gene	lacZ, beta-galactosidase, E. coli
Site of Expression	This is a promoter-less negative control strain for Pleiades strain expressing lacZ. No lacZ expression is evident.

Genetics

Hprt^{tm37(lacZ)Ems}

Allele Symbol: Hprt^{tm37(lacZ)Ems}

Allele Name	targeted mutation 37, Elizabeth M Simpson
Allele Type	Targeted
Allele Synonym(s)	Hprt^{tm37(mEMS3689)Ems}; Ptomoterless-lacZ-mEMA3689
Gene Symbol and Name	Hprt, hypoxanthine guanine phosphoribosyl transferase
Gene Synonym(s)
Expressed Gene	lacZ, beta-galactosidase, E. coli
Site of Expression	This is a promoter-less negative control strain for Pleiades strain expressing lacZ. No lacZ expression is evident.
Strain of Origin	(B6.129P2-Hprt^b-m3/J x 129S-Gt(ROSA)26Sor^tm1Sor/J)F1
Chromosome	X
General Note	Germ line transmission of mutant cell line mEMS3689 has been established.
Molecular Note	The "Promoterless-lacZ" transgene (pEMS1313) was designed with no Pleiades minipromoter upstream of a frt-flanked _-galactosidase (lacZ) gene, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprt^b-m3 mutant locus on the X chromosome. Without a promoter directing expression of beta-galactosidase (lacZ), no lacZ expression pattern is seen or anticipated.
Mutations Made By	Elizabeth Simpson, Centre for Molecular Medicine & Therapeutics, University of British Columbia

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Neurobiology Research
- lacZ expression in neural tissue
Research Tools
- lacZ Expression

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Hprt^{tm37(lacZ)Ems}/Y
involves: 129P2/OlaHsd * 129S4/SvJaeSor * C57BL/6J

normal phenotype

no abnormal phenotype detected
- Normal - heterozygous females and hemizygous males are viable and fertile

References

Additional - Hprt^{tm37(lacZ)Ems} related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Probe:Gt(rosa)26sor Probe
- Standard PCR:Hprt1
- Genotyping resources and troubleshooting
Breeding Considerations

The donating investigator recommends maintaining this strain by breeding heterozygous females with C57BL/6J inbred males.
- Additional Breeding and Husbandry Support
Citation
When using the STOCK Hprt^{tm37(lacZ)Ems}/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #32935 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

See MMRRC for Additional Conditions of Distribution

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Donating Investigator

Genetic overview

Research Applications

Expression Data

Hprttm37(lacZ)Ems

Allele Symbol: Hprttm37(lacZ)Ems

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Hprttm37(lacZ)Ems/Yinvolves: 129P2/OlaHsd * 129S4/SvJaeSor * C57BL/6J

normal phenotype

References

Additional - Hprttm37(lacZ)Ems related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

Hprt^{tm37(lacZ)Ems}

Allele Symbol: Hprt^{tm37(lacZ)Ems}

Genotype: Hprt^{tm37(lacZ)Ems}/Y
involves: 129P2/OlaHsd * 129S4/SvJaeSor * C57BL/6J

Additional - Hprt^{tm37(lacZ)Ems} related