C;129S-Adora2a^tm1Jfc/J

Stock number: 010685
Product name: A_2A KO
Strain synonyms: A2AAR-, A2aR-, A2AR KO
Research areas: Cardiovascular Research, Immunology, Inflammation and Autoimmunity Research, Neurobiology Research, Research Tools

Description

Mice that are homozygous for this knockout of the Adora2a gene, are resistant to experimentally induced ischemic brain injury and exhibit increased resistance to the addictive substances amphetamine and cocaine.

Detailed description

Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No functional gene product (protein) is detected by receptor autoradiography analysis of the brain. Experimentally induced cerebral infarction volume and resulting neurological function impairment are reduced in homozygotes. Treatment with receptor agonist, CGS 21680, does not elicit decreased locomotor activity in homozygotes, as compared to wildtype. Homozygotes have reduced spontaneous activity and increased resistance to the addictive substances amphetamine and cocaine. This mutant mouse strain may be useful in studies of behavior, ischemic brain injury, stroke, responses to addictive substances and T cell response to inflammation.

Development

A targeting vector containing PGKneo cassette was used to disrupt the 3' portion of exon 2 and intron 2. The construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to 129/SvEvTac mice, and then been backcrossed to BALB/cJ for 2 generations (using a speed congenic protocol) before arriving at The Jackson Laboratory. The mice were crossed to BALB/cJ once to establish the colony.

Allele

Symbol: Adora2a^tm1Jfc
Name: targeted mutation 1, Jiang-Fan Chen
MGI accession ID: MGI:2155985
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Adora2a
Marker name: adenosine A2a receptor
Chromosome: 10
Strain of origin: 129S4/SvJae
Molecular note: Part of exon 2 and intron 2 was replaced by a neomycin selection cassette. The absence of functional protein was demonstrated in homozygous mice by in situ binding assays and neurochemical tests.