These mice harbor a targeted mutation of the Gt(ROSA)26Sor locus with a loxP-flanked neo cassette preventing transcription of a CAG promoter-driven tau- beta galactosidase (tauLacZ) gene, and may be useful as a cre reporter strain. Beta galactosidase is expressed following cre-mediated recombination.
Robert Benezra, Memorial Sloan Kettering Cancer Center
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), Reporter) | Gt(ROSA)26Sor | gene trap ROSA 26, Philippe Soriano |
Mice that are homozygous for the targeted mutation are viable, fertile and normal in size. A loxP-flanked neo cassette prevents transcription of the downstream tau- beta galactosidase (tauLacZ) gene. When bred to mice that express Cre recombinase, the resulting offspring will have the neo cassette deleted in the cre-expressing tissue(s); resulting in expression of taulacZ. Upon histochemical staining with X-gal, the tau beta galactosidase activity is revealed as an intense blue precipitate found in the entire cell, regardless of cell type. When tested with an olfactory sensory neuron specific cre-expressing strain, expression of lacZ labels axon projections. This mutant mouse strain may be useful as a cre reporter.
A targeting vector containing a CAG promoter, a loxP flanked neomycin resistance gene followed by a tau-lacZ fusion gene and a polyadenylation sequence (CAG-loxP-neo-pA-loxP-tauLacZ-pA) was inserted in reverse orientation into the Gt(ROSA)26 locus. The construct was electroporated into B6(Cg)-Tyrc-2J derived CY2.4 embryonic stem (ES) cells, correctly targeted ES cells were injected into recipient C57BL/6 blastocysts and chimeric males were bred with B6(Cg)-Tyrc-2J females to generate the first generation. The resulting mice were crossed to C57BL/6J mice and bred to homozygosity. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Expressed Gene | neo, neomycin resistance, bacterial |
Site of Expression | A loxP-flanked neo cassette prevents transcription of tauLacZ. When bred to mice that express Cre recombinase, tissue-specific expression of taulacZ is enabled. |
Allele Name | targeted mutation 1, Robert Benezra |
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Allele Type | Targeted (Conditional ready (e.g. floxed), Reporter) |
Allele Synonym(s) | Gt(ROSA)26Sortm1(CAG-MAPT/lacZ)Bene; Gt(ROSA)26Sortm1(CAG-taulacZ)Bene; ROSA26StLa; StLa; Stop-tauLacZ |
Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano |
Gene Synonym(s) | |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Expressed Gene | neo, neomycin resistance, bacterial |
Site of Expression | A loxP-flanked neo cassette prevents transcription of tauLacZ. When bred to mice that express Cre recombinase, tissue-specific expression of taulacZ is enabled. |
Strain of Origin | B6(Cg)-Tyrc-2J |
Chromosome | 6 |
Molecular Note | A genetic construct that reports cre recombinase activity with tauLacZ reporter gene was inserted in reverse orientation into the Gt(ROSA)26Sor safe harbor locus. The reporter construct included a CAG promoter, a loxP flanked stop cassette composed of neomycin resistance gene and multiple polyadenylation sequences, a tauLacZ reporter gene, and a polyadenylation sequence (pCAG-loxP-pEM7-Neo*-bGHpA-3xSV40pA-loxP-tauLacZ-bGHpA). The construct was electroporated into B6(Cg)-Tyrc-2J derived CY2.4 embryonic stem cells. Southern blot positive embryonic stem cell clones were injected into recipient C57BL/6J blastocysts. Positive male chimeras were bred with B6(Cg)-Tyrc-2J females. Albino pups were genotyped by PCR, then bred to B6J mice. |
While maintaining a live colony, these mice are bred as homozygotes. The donating investigator indicates that some white mice are still observed (the result of a cross to B6(Cg)-Tyrc-2J/J) in this strain.
When using the B6(Cg)-Gt(ROSA)26Sortm1(CAG-taulacZ)Bene/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #010633 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Gt(ROSA)26Sor<tm1(CAG-taulacZ)Bene> |
Frozen Mouse Embryo | B6(Cg)-Gt(ROSA)26Sor<tm1(CAG-taulacZ)Bene>/J | $2595.00 |
Frozen Mouse Embryo | B6(Cg)-Gt(ROSA)26Sor<tm1(CAG-taulacZ)Bene>/J | $2595.00 |
Frozen Mouse Embryo | B6(Cg)-Gt(ROSA)26Sor<tm1(CAG-taulacZ)Bene>/J | $3373.50 |
Frozen Mouse Embryo | B6(Cg)-Gt(ROSA)26Sor<tm1(CAG-taulacZ)Bene>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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