NOD.ALR-(D8Mit205-D8Mit137)/MxJ

Stock number: 010613
Product name: NOD.ALR-Idd22
Research areas: Diabetes and Obesity Research, Immunology, Inflammation and Autoimmunity Research

Description

This chromosome 8 congenic strain, commonly called NOD.ALRc8 or NOD-Idd22^ALR/Lt is diabetes and alloxan resistant. This strain may be useful to further study the role of oxidative stress in autoimmune diabetes and to identify diabetes resistant candidate genes in the Idd22 region.

Detailed description

This diabetes resistant NOD congenic strain, commonly called NOD.ALRc8 or NOD-Idd22^ALR/Lt, contains a unique diabetes resistant ALR/Lt derived genomic segment on Chr. 8 extending from D8Mit205 (51.9Mb) through D8Mit137 (106Mb) and contains Idd22 (90.6Mb). NOD.ALRc8 mice treated with 45mg/kg of Alloxan are completely diabetes resistant seven days post injection, unlike male NOD/ShiLt controls that are diabetes susceptible at this dose. In contrast to ALR/Lt males, which are diabetes resistant at doses higher than 65mg/kg, the NOD.ALRc8 male mice are diabetes susceptible at doses of 50mg/kg. This strain may be useful to further study the role of oxidative stress in autoimmune diabetes and to identify diabetes resistant candidate genes in the Idd22 region.

Development

This NOD congenic strain contains a genomic segment from the diabetes and alloxan (a pancreatic beta cell-selective toxin) resistant, ALR/Lt introgressed into Chromosome 8. ALR/Lt and NOD/ShiLt share 70-85% genetic identity including MHC class II alleles. The chromosome 8 genomic segment from ALR/Lt extends from D8Mit205 (51.9Mb) through D8Mit137 (106Mb) and contains a unique ALR/Lt diabetes resistant allele, Idd22. The ALR/Lt genomic segment was transferred through 10 generations of backcrossing to NOD/ShiLt. Siblings carrying the ALR/Lt derived segments denoted by markers D8Mit205 through D8Mit137 and were intercrossed to make homozygous. Additional microsatellite analysis confirms all tested Idd alleles (1-15) are of NOD/ShiLt origin. In 2010, the T1DR received this strain at generation N11F3

Allele

Symbol: Idd22^ALR/Lt
Name: ALR/Lt
MGI accession ID: MGI:2683527
Generation method: QTL
Marker symbol: Idd22
Marker name: insulin dependent diabetes susceptibility 22
Chromosome: 8
Strain of origin: ALR/Lt
Molecular note: This allele confers resistance to type 1 diabetes mellitus compared to NOD/Lt.

Allele

MGI accession ID: MGI:100205
Marker symbol: D8Mit205
Marker name: DNA segment, Chr 8, Massachusetts Institute of Technology 205
Chromosome: 8

Allele

MGI accession ID: MGI:94317
Marker symbol: D8Mit137
Marker name: DNA segment, Chr 8, Massachusetts Institute of Technology 137
Chromosome: 8