This chromosome 8 congenic strain, commonly called NOD.ALRc8 or NOD-Idd22ALR/Lt is diabetes and alloxan resistant. This strain may be useful to further study the role of oxidative stress in autoimmune diabetes and to identify diabetes resistant candidate genes in the Idd22 region.
Dr. Clayton Mathews, University of Florida
|Allele Type||Gene Symbol||Gene Name|
|QTL||Idd22||insulin dependent diabetes susceptibility 22|
|Marker Symbol||Marker Name|
|D8Mit205||DNA segment, Chr 8, Massachusetts Institute of Technology 205|
|D8Mit137||DNA segment, Chr 8, Massachusetts Institute of Technology 137|
The NOD.ALR stock at The Jackson Laboratory containing the D8Mit205 through D8Mit137 of ALR/J origin becomes diabetic at a rate similar to NOD. The donating investigator maintains a stock NOD.ALR containing D8Mit293 through D8Mit137 from ALR/J that does not become diabetic. It is thought that Idd22 is between D8Mit293 andD8Mit205. D8Mit293 in this stock is of NOD origin.
This diabetes resistant NOD congenic strain, commonly called NOD.ALRc8 or NOD-Idd22ALR/Lt, contains a unique diabetes resistant ALR/Lt derived genomic segment on Chr. 8 extending from D8Mit205 (51.9Mb) through D8Mit137 (106Mb) and contains Idd22 (90.6Mb).
NOD.ALRc8 mice treated with 45mg/kg of Alloxan are completely diabetes resistant seven days post injection, unlike male NOD/ShiLt controls that are diabetes susceptible at this dose. In contrast to ALR/Lt males, which are diabetes resistant at doses higher than 65mg/kg, the NOD.ALRc8 male mice are diabetes susceptible at doses of 50mg/kg.
This strain may be useful to further study the role of oxidative stress in autoimmune diabetes and to identify diabetes resistant candidate genes in the Idd22 region.
This NOD congenic strain contains a genomic segment from the diabetes and alloxan (a pancreatic beta cell-selective toxin) resistant, ALR/Lt introgressed into Chromosome 8. ALR/Lt and NOD/ShiLt share 70-85% genetic identity including MHC class II alleles. The chromosome 8 genomic segment from ALR/Lt extends from D8Mit205 (51.9Mb) through D8Mit137 (106Mb) and contains a unique ALR/Lt diabetes resistant allele, Idd22. The ALR/Lt genomic segment was transferred through 10 generations of backcrossing to NOD/ShiLt. Siblings carrying the ALR/Lt derived segments denoted by markers D8Mit205 through D8Mit137 and were intercrossed to make homozygous. Additional microsatellite analysis confirms all tested Idd alleles (1-15) are of NOD/ShiLt origin. In 2010, the T1DR received this strain at generation N11F3
When maintaining a live colony, these mice can be bred as homozygotes.
When using the NOD.ALR-Idd22 mouse strain in a publication, please cite the originating article(s) and include JAX stock #010613 in your Materials and Methods section.