Overview

Myrf^tm1Barr mice harbor loxP sites flanking exon 8 of the predicted gene 98 gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region deleted in the cre-expressing tissue(s). These mice may be useful in generating conditional mutations for studying the role of Myrf in CNS myelination and other cellular processes.

Donating Investigator

Ben A. Barres, Stanford University

Genetic overview

Genetic Background	Generation

Myrf^tm1Barr

Allele Type	Gene Symbol	Gene Name
Targeted (Conditional ready (e.g. floxed), No functional change)	Myrf	myelin regulatory factor

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Research Applications

Neurobiology Research
Research Tools

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

Mice that are homozygous for the targeted mutation are viable, fertile with loxP sites flanking exon 8 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region deleted in cre-expressing tissue(s). This mutant mouse strain may be useful in generating conditional mutations for studying the role of Gm98 in CNS myelination and other cellular processes.

For example, when crossed to a strain expressing Cre recombinase in oligodendrocytes(see Stock No. 011103), this mutant mouse strain may be useful in studies of oligodendrocyte maturation and CNS myelination.

Development

A targeting vector was used to flank exon 8 of the endogenous gene with loxP sites. The vector also contained an FRT-flanked neomycin resistance cassette. The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice. Heterozygous mice were crossed to the FlpER (129S4/SvJaeSor-Gt(ROSA)26Sor^tm1(FLP1)Dym) strain to remove the neo cassette, leaving a single FRT site upstream of exon 8 and the flanking loxP sites. The resulting mutant mice were interbred to generate homozygotes and maintained on a mixed B6;129P2 background prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred to C57BL/6J for at least one generation to establish the colony.

Control Suggestions

Approximate Controls

100903 B6129PF2/J

Additional Information

Considerations for Choosing Controls

Selected References

Emery B; Agalliu D; Cahoy JD; Watkins TA; Dugas JC; Mulinyawe SB; Ibrahim A; Ligon KL; Rowitch DH; Barres BA. 2009. Myelin gene regulatory factor is a critical transcriptional regulator required for CNS myelination. Cell 138(1):172-85PubMed: 19596243 MGI: J:150563

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Genetics

Myrf^tm1Barr

Allele Symbol: Myrf^tm1Barr

Allele Name	targeted mutation 1, Ben Barres
Allele Type	Targeted (Conditional ready (e.g. floxed), No functional change)
Allele Synonym(s)	targeted mutation 1, Ben Barres; Myrf^tm1Barr
Gene Symbol and Name	Myrf, myelin regulatory factor
Gene Synonym(s)	Gm1804; C11orf9; RGD1306622; gene model 1804, (NCBI); MRF; Gm98; LOC225908; pqn-47; Ndt80; Gm98; gene model 98, (NCBI); LOC386531; predicted gene 98; Mrf; MMERV; 11orf9; CUGS
Strain of Origin	129P2/OlaHsd
Chromosome	19
Molecular Note	A targeting vector was used to flank exon 8 of the endogenous gene with loxP sites. The vector also contained an FRT-flanked neomycin resistance cassette. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice. Heterozygous mice were crossed to the FlpER (129S4/SvJaeSor-Gt(ROSA)26Sor^tm1(FLP1)Dym) strain to remove the neo cassette, leaving a single FRT site upstream of exon 8 and the flanking loxP sites.
Mutations Made By	Ben Barres, Stanford University

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Neurobiology Research
- Myelination Defects
- Cre-lox System
  - loxP-flanked Sequences
Research Tools
- Cre-lox System
  - loxP-flanked Sequences

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Myrf^tm1Barr/Myrf^tm1Barr Olig2^{tm2(TVA,cre)Rth}/Olig2⁺
involves: 129P2/OlaHsd * 129S4/SvJaeSor * C57BL/6

behavior/neurological phenotype

ataxia
- observed around P11

seizures
- develop after P10

tremors
- severe tremors are observed around P11

vision/eye phenotype

abnormal optic nerve morphology
- at P13, optic nerve axons show almost no myelination in contrast to wild-type controls where about 50% of axons are myelinated

mammalian phenotype

nervous system phenotype
- Normal - gross CNS architecture is normal
- Normal - motor neuron population in spinal cord is intact
- Normal - peripheral nerves are fully myelinated

mortality/aging

postnatal lethality, complete penetrance
- mice die during third postnatal week

nervous system phenotype

abnormal myelination
- essentially complete loss of myelination in CNS white matter tracts is observed at P13

abnormal optic nerve morphology
- at P13, optic nerve axons show almost no myelination in contrast to wild-type controls where about 50% of axons are myelinated

abnormal spinal cord morphology
- severe loss of myelin basic protein (immunostaining) is observed in spinal cord at P13, but spinal root myelination (myelinated by Schwann cells) is normal

seizures
- develop after P10

References

Emery B; Agalliu D; Cahoy JD; Watkins TA; Dugas JC; Mulinyawe SB; Ibrahim A; Ligon KL; Rowitch DH; Barres BA. 2009. Myelin gene regulatory factor is a critical transcriptional regulator required for CNS myelination. Cell 138(1):172-85PubMed: 19596243 MGI: J:150563

Additional - Myrf^tm1Barr related

Technical Support

Contact Technical Support

Genotyping Protocols
- Separated PCR:Myrf^tm1Barr
- Genotyping resources and troubleshooting
Breeding Considerations

While maintaining a live colony, these mice are bred as homozygotes.
- Additional Breeding and Husbandry Support
Citation
When using the B6;129-Myrf^tm1Barr/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #010607 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous for Gm98<tm1Barr>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Related Products and Services

Frozen Mouse Embryo	B6;129-Myrf<tm1Barr>/J Frozen Embryo	$2595.00
Frozen Mouse Embryo	B6;129-Myrf<tm1Barr>/J Frozen Embryo	$2595.00
Frozen Mouse Embryo	B6;129-Myrf<tm1Barr>/J Frozen Embryo	$3373.50
Frozen Mouse Embryo	B6;129-Myrf<tm1Barr>/J Frozen Embryo	$3373.50

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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General Terms and Conditions

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

JAX® Mice, Products & Services Conditions of Use

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In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

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The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.

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Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Approximate Controls

Additional Information

Selected References

Myrftm1Barr

Allele Symbol: Myrftm1Barr

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Myrftm1Barr/Myrftm1Barr Olig2tm2(TVA,cre)Rth/Olig2+involves: 129P2/OlaHsd * 129S4/SvJaeSor * C57BL/6

behavior/neurological phenotype

vision/eye phenotype

mammalian phenotype

mortality/aging

nervous system phenotype

References

Additional - Myrftm1Barr related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Myrf^tm1Barr

Allele Symbol: Myrf^tm1Barr

Genotype: Myrf^tm1Barr/Myrf^tm1Barr Olig2^{tm2(TVA,cre)Rth}/Olig2⁺
involves: 129P2/OlaHsd * 129S4/SvJaeSor * C57BL/6

Additional - Myrf^tm1Barr related