B6;129-Myrf^tm1Barr/J

Stock number: 010607
Product name: MRF floxed
Research areas: Neurobiology Research, Research Tools

Description

Myrf^tm1Barr mice harbor loxP sites flanking exon 8 of the myelin regulatory factor gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region deleted in the cre-expressing tissue(s). These mice may be useful in generating conditional mutations for studying the role of Myrf in CNS myelination and other cellular processes.

Detailed description

Mice that are homozygous for the targeted mutation are viable, fertile with loxP sites flanking exon 8 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region deleted in cre-expressing tissue(s). This mutant mouse strain may be useful in generating conditional mutations for studying the role of Myrf in CNS myelination and other cellular processes.

For example, when crossed to a strain expressing Cre recombinase in oligodendrocytes (see Stock No. 011103), this mutant mouse strain may be useful in studies of oligodendrocyte maturation and CNS myelination.

Development

A targeting vector was used to flank exon 8 of the endogenous gene with loxP sites. The vector also contained an FRT-flanked neomycin resistance cassette. The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice. Heterozygous mice were crossed to the FlpER (129S4/SvJaeSor-Gt(ROSA)26Sor^tm1(FLP1)Dym) strain to remove the neo cassette, leaving a single FRT site upstream of exon 8 and the flanking loxP sites. The resulting mutant mice were interbred to generate homozygotes and maintained on a mixed B6;129P2 background prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred to C57BL/6J for at least one generation to establish the colony.

Allele

Symbol: Myrf^tm1Barr
Name: targeted mutation 1, Ben Barres
MGI accession ID: MGI:3851143
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Myrf
Marker name: myelin regulatory factor
Chromosome: 19
Strain of origin: 129P2/OlaHsd
Molecular note: A targeting vector was used to flank exon 8 of the endogenous gene with loxP sites. The vector also contained an FRT-flanked neomycin resistance cassette. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice. Heterozygous mice were crossed to the FlpER (129S4/SvJaeSor-Gt(ROSA)26Sor^tm1(FLP1)Dym) strain to remove the neo cassette, leaving a single FRT site upstream of exon 8 and the flanking loxP sites.