Overview

This strain is currently unavailable due to replenishing of cryopreserved stocks. Interested customers can register interest.

Myrf^tm1Barr mice harbor loxP sites flanking exon 8 of the predicted gene 98 gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region deleted in the cre-expressing tissue(s). These mice may be useful in generating conditional mutations for studying the role of Myrf in CNS myelination and other cellular processes.

Donating Investigator

Ben A. Barres, Stanford University

Genetic overview

Genetic Background	Generation

Myrf^tm1Barr

Allele Type	Gene Symbol	Gene Name
Targeted (Conditional ready (e.g. floxed), No functional change)	Myrf	myelin regulatory factor

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Research Applications

Research Tools
Neurobiology Research

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Details

Detailed Description

Mice that are homozygous for the targeted mutation are viable, fertile with loxP sites flanking exon 8 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region deleted in cre-expressing tissue(s). This mutant mouse strain may be useful in generating conditional mutations for studying the role of Gm98 in CNS myelination and other cellular processes.

For example, when crossed to a strain expressing Cre recombinase in oligodendrocytes(see Stock No. 011103), this mutant mouse strain may be useful in studies of oligodendrocyte maturation and CNS myelination.

Development

A targeting vector was used to flank exon 8 of the endogenous gene with loxP sites. The vector also contained an FRT-flanked neomycin resistance cassette. The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice. Heterozygous mice were crossed to the FlpER (129S4/SvJaeSor-Gt(ROSA)26Sor^tm1(FLP1)Dym) strain to remove the neo cassette, leaving a single FRT site upstream of exon 8 and the flanking loxP sites. The resulting mutant mice were interbred to generate homozygotes and maintained on a mixed B6;129P2 background prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred to C57BL/6J for at least one generation to establish the colony.

Control Suggestions

Approximate Controls

100903 B6129PF2/J

Additional Information

Considerations for Choosing Controls

Selected References

Emery B; Agalliu D; Cahoy JD; Watkins TA; Dugas JC; Mulinyawe SB; Ibrahim A; Ligon KL; Rowitch DH; Barres BA. 2009. Myelin gene regulatory factor is a critical transcriptional regulator required for CNS myelination. Cell 138(1):172-85PubMed: 19596243 MGI: J:150563

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Genetics

Myrf^tm1Barr

Allele Symbol: Myrf^tm1Barr

Allele Name	targeted mutation 1, Ben Barres
Allele Type	Targeted (Conditional ready (e.g. floxed), No functional change)
Allele Synonym(s)	MRF^fl; Myrf^loxP
Gene Symbol and Name	Myrf, myelin regulatory factor
Gene Synonym(s)
Strain of Origin	129P2/OlaHsd
Chromosome	19
Molecular Note	A targeting vector was used to flank exon 8 of the endogenous gene with loxP sites. The vector also contained an FRT-flanked neomycin resistance cassette. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice. Heterozygous mice were crossed to the FlpER (129S4/SvJaeSor-Gt(ROSA)26Sor^tm1(FLP1)Dym) strain to remove the neo cassette, leaving a single FRT site upstream of exon 8 and the flanking loxP sites.
Mutations Made By	Ben Barres, Stanford University

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- Cre-lox System
  - loxP-flanked Sequences
Neurobiology Research
- Cre-lox System
  - loxP-flanked Sequences
- Myelination Defects

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Myrf^tm1Barr/Myrf^tm1Barr Olig2^{tm2(TVA,cre)Rth}/Olig2⁺
involves: 129P2/OlaHsd * 129S4/SvJaeSor * C57BL/6

behavior/neurological phenotype

ataxia
- observed around P11

seizures
- develop after P10

tremors
- severe tremors are observed around P11

vision/eye phenotype

abnormal optic nerve morphology
- at P13, optic nerve axons show almost no myelination in contrast to wild-type controls where about 50% of axons are myelinated

mammalian phenotype

nervous system phenotype
- Normal - motor neuron population in spinal cord is intact
- Normal - peripheral nerves are fully myelinated
- Normal - gross CNS architecture is normal

mortality/aging

postnatal lethality, complete penetrance
- mice die during third postnatal week

nervous system phenotype

abnormal myelination
- essentially complete loss of myelination in CNS white matter tracts is observed at P13

abnormal optic nerve morphology
- at P13, optic nerve axons show almost no myelination in contrast to wild-type controls where about 50% of axons are myelinated

abnormal spinal cord morphology
- severe loss of myelin basic protein (immunostaining) is observed in spinal cord at P13, but spinal root myelination (myelinated by Schwann cells) is normal

seizures
- develop after P10

References

Emery B; Agalliu D; Cahoy JD; Watkins TA; Dugas JC; Mulinyawe SB; Ibrahim A; Ligon KL; Rowitch DH; Barres BA. 2009. Myelin gene regulatory factor is a critical transcriptional regulator required for CNS myelination. Cell 138(1):172-85PubMed: 19596243 MGI: J:150563

Additional - Myrf^tm1Barr related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Separated PCR:Myrf-alternate2
- Genotyping resources and troubleshooting
Breeding Considerations

While maintaining a live colony, these mice are bred as homozygotes.
- Additional Breeding and Husbandry Support
Citation
When using the B6;129-Myrf^tm1Barr/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #010607 in your Materials and Methods section.

Strain Interest Registration

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This information helps us manage the colony build and better meet the broad needs of the research community.
Please send any technical questions to Technical Support.

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Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Donating Investigator

Genetic overview

Research Applications

Detailed Description

Development

Control Suggestions

Approximate Controls

Additional Information

Selected References

Myrftm1Barr

Allele Symbol: Myrftm1Barr

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Myrftm1Barr/Myrftm1Barr Olig2tm2(TVA,cre)Rth/Olig2+involves: 129P2/OlaHsd * 129S4/SvJaeSor * C57BL/6

behavior/neurological phenotype

vision/eye phenotype

mammalian phenotype

mortality/aging

nervous system phenotype

References

Additional - Myrftm1Barr related

Genotyping Protocols

Breeding Considerations

Citation

Strain Interest Registration

Contact Information

Interest

Terms Of Use

Licensing Information

Myrf^tm1Barr

Allele Symbol: Myrf^tm1Barr

Genotype: Myrf^tm1Barr/Myrf^tm1Barr Olig2^{tm2(TVA,cre)Rth}/Olig2⁺
involves: 129P2/OlaHsd * 129S4/SvJaeSor * C57BL/6

Additional - Myrf^tm1Barr related