Ghrhhpg Prkdcscid double mutant mice are useful for determining endocrine dependence of grafted cells/tissues.
Wes Beamer, JAX
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Spontaneous | Gnrh1 | gonadotropin releasing hormone 1 |
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Spontaneous | Prkdc | protein kinase, DNA activated, catalytic polypeptide |
Mice homozygous for the severe combined immune deficiency spontaneous mutation (Prkdcscid, commonly referred to as scid) are characterized by an absence of functional T cells and B cells, lymphopenia, hypogammaglobulinemia, and a normal hematopoietic microenvironment. Normal antigen-presenting cell, myeloid and NK cell functions are strain dependent. scid mice carry a DNA repair defect and a defect in the rearrangement of genes that code for antigen-specific receptors on lymphocytes. Most homozygotes have no detectable IgM, IgG1, IgG2a, IgG2b, IgG3, or IgA. Thymus, lymph nodes, and splenic follicles are virtually devoid of lymphocytes. scid mice accept allogeneic and xenogeneic grafts making them an ideal model for cell transfer experiments. Some scid mice will spontaneously develop partial immune reactivity. scid mice that have serum Ig levels greater than 1 ug/ml are considered "leaky." scid leakiness is highly strain dependent, increases with age, and is higher in mice housed under non SPF conditions. In general, scid leakiness is high on the C57BL/6J and BALB/cBy genetic backgrounds, low on the C3H/HeJ background, and even lower on the NOD/LtSz background. Hypogonadal mice (Ghrhhpg) mice are deficient in gonadotropins and gonodal steroids leading to an overall underdevelopment of the reproductive tract. Male mice have a small penis and scrotum and a short ano-genital distance. All male reproductive organs are present but immature. The testes are small and undescended. Spermatogenesis is arrested, usually by the diplotene stage. The vagina of female mice does not open fully, the uterus is thread-like, and the ovaries very small. Both sexes are sterile although fertility can be restored with hormone replacement. Ghrhhpg Prkdcscid double mutant mice are useful for determining endocrine dependence of grafted cells/tissues.
A mouse homozygous for the Prkdcscid and Ighb mutations on the CB17/IcrSmn background was bred with an HPG/BmJ mouse and the offspring were intercrossed to generate a line on a segregating background, homozygous for Prkdcscid and segregating for Gnrh1hpg. This strain (stock #002038) was maintained by intercrossing siblings homozygous for the scid mutation and heterozygous for the hpg mutation. In 2009, when this strain reached generation F34, sperm were cryopreserved from a male homozygous for scid and heterozygous for hpg. Cryorecovery will use CBySmn.CB17-Prkdcscid/J females (stock #001803).
Allele Name | hypogonadal |
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Allele Type | Spontaneous |
Allele Synonym(s) | Gnrh-; gnrhhpg; hpg |
Gene Symbol and Name | Gnrh1, gonadotropin releasing hormone 1 |
Gene Synonym(s) | |
Strain of Origin | C3H101H-Rb(1.3)1Bnr |
Chromosome | 14 |
Molecular Note | The mutation in the hypogonadal mouse is an approximately 33.5 kb deletion that removes two exons that encode most of the protein. |
Allele Name | severe combined immunodeficiency |
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Allele Type | Spontaneous |
Allele Synonym(s) | SCID |
Gene Symbol and Name | Prkdc, protein kinase, DNA activated, catalytic polypeptide |
Gene Synonym(s) | |
Site of Expression | T and B lymphocytes. |
Strain of Origin | C.BKa-Ighb/Icr |
Chromosome | 16 |
Molecular Note | A T-to-A transversion point mutation at a position corresponding to codon 4046 (codon 4095 in transcript ENSMUST00000023352.8) created a premature stop codon (p.Y4046*). |
When using the STOCK Prkdcscid Gnrh1hpg/BmJ mouse strain in a publication, please cite the originating article(s) and include JAX stock #010605 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Homozygous for Prkdc<scid>, heterozygous or wild-type for Gnrh1<hpg> |
Frozen Mouse Embryo | STOCK Prkdc<scid> Gnrh1<hpg>/BmJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Prkdc<scid> Gnrh1<hpg>/BmJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Prkdc<scid> Gnrh1<hpg>/BmJ Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Prkdc<scid> Gnrh1<hpg>/BmJ Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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