These Nfatc3 (nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3) knock-out mutant mice have impaired development of CD4 and CD8 thymocytes. These mice also show many physiological and pathological effects and may be useful in many areas of research including T cell development and survival.
Dr. Laurie H. Glimcher, Dana Farber Cancer Institute
Jeffery D. Molkentin, Cincinnati Children's Hospital
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Nfatc3 | nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 3 |
Expression of the targeted gene is eliminated in this strain. Mice have impaired development of both CD4 and CD8 single positive thymocytes (40-60% decrease). Peripheral T cell are reduced in number and are hyperactivated. An increase in the apoptosis of CD4/CD8 double positive thymocytes and a reduction in the number of single positive CD4 and CD8 cells in the spleen are also characteristic. This strain may be useful in studies of T cell development and survival.
The sequence corresponding to amino acids 535-592 was replaced with a neomycin resistance cassette in 129S2/SvPas-derived D3 embryonic stem (ES) cells. The strain was backcrossed to C57BL/6 at least three times by the donating laboratory.
Allele Name | targeted mutation 1, Laurie H Glimcher |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | c3-; c3-KO; NFAT4-; nfatc3-; Nfatc4 - |
Gene Symbol and Name | Nfatc3, nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 3 |
Gene Synonym(s) | |
Strain of Origin | 129S2/SvPas |
Chromosome | 8 |
Molecular Note | Sequence encoding the DNA-binding domain, encoding amino acids 535 through 592, was replaced with a neomycin selection cassette. Encoded protein was undetected via Western blot analysis of thymus protein extracts obtained from homozygous mutant mice. |
Mutations Made By | Dr. Laurie Glimcher, Dana Farber Cancer Institute |
When maintained in a live colony, heterozygous females may be bred to homozygous males. Homozygous females are reported not to breed well.
When using the NFAT4 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #010589 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Nfatc3<tm1Glm> |
Frozen Mouse Embryo | B6;129S2-Nfatc3<tm1Glm>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129S2-Nfatc3<tm1Glm>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129S2-Nfatc3<tm1Glm>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129S2-Nfatc3<tm1Glm>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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