These Nfatc3 (nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3) knock-out mutant mice have impaired development of CD4 and CD8 thymocytes. These mice also show many physiological and pathological effects and may be useful in many areas of research including T cell development and survival.
Dr. Laurie H. Glimcher, Dana Farber Cancer Institute
Jeffery D. Molkentin, Cincinnati Children's Hospital
Expression of the targeted gene is eliminated in this strain. Mice have impaired development of both CD4 and CD8 single positive thymocytes (40-60% decrease). Peripheral T cell are reduced in number and are hyperactivated. An increase in the apoptosis of CD4/CD8 double positive thymocytes and a reduction in the number of single positive CD4 and CD8 cells in the spleen are also characteristic. This strain may be useful in studies of T cell development and survival.
The sequence corresponding to amino acids 535-592 was replaced with a neomycin resistance cassette in 129S2/SvPas-derived D3 embryonic stem (ES) cells. The strain was backcrossed to C57BL/6 at least three times by the donating laboratory.
|Allele Name||targeted mutation 1, Laurie H Glimcher|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||c3-; c3-KO; NFAT4-; nfatc3-; Nfatc4 -|
|Gene Symbol and Name||Nfatc3, nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 3|
|Strain of Origin||129S2/SvPas|
|Molecular Note||Sequence encoding the DNA-binding domain, encoding amino acids 535 through 592, was replaced with a neomycin selection cassette. Encoded protein was undetected via Western blot analysis of thymus protein extracts obtained from homozygous mutant mice.|
|Mutations Made By|| |
Dr. Laurie Glimcher, Dana Farber Cancer Institute
When maintained in a live colony, heterozygous females may be bred to homozygous males. Homozygous females are reported not to breed well.
When using the NFAT4 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #010589 in your Materials and Methods section.