B6;129S2-Nfatc3^tm1Glm/J

Stock number: 010589
Product name: NFAT4 KO
Research areas: Immunology, Inflammation and Autoimmunity Research

Description

These Nfatc3 (nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3) knock-out mutant mice have impaired development of CD4 and CD8 thymocytes. These mice also show many physiological and pathological effects and may be useful in many areas of research including T cell development and survival.

Detailed description

Expression of the targeted gene is eliminated in this strain. Mice have impaired development of both CD4 and CD8 single positive thymocytes (40-60% decrease). Peripheral T cell are reduced in number and are hyperactivated. An increase in the apoptosis of CD4/CD8 double positive thymocytes and a reduction in the number of single positive CD4 and CD8 cells in the spleen are also characteristic. This strain may be useful in studies of T cell development and survival.

Development

The sequence corresponding to amino acids 535-592 was replaced with a neomycin resistance cassette in 129S2/SvPas-derived D3 embryonic stem (ES) cells. The strain was backcrossed to C57BL/6 at least three times by the donating laboratory.

Allele

Symbol: Nfatc3^tm1Glm
Name: targeted mutation 1, Laurie H Glimcher
MGI accession ID: MGI:2182778
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Nfatc3
Marker name: nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 3
Chromosome: 8
Strain of origin: 129S2/SvPas
Molecular note: Sequence encoding the DNA-binding domain, encoding amino acids 535 through 592, was replaced with a neomycin selection cassette. Encoded protein was undetected via Western blot analysis of thymus protein extracts obtained from homozygous mutant mice.