B6;SJL-Tg(MMTV-rtTA)4-1Jek/J

Stock number: 010576
Research areas: Endocrine Deficiency Research, Neurobiology Research, Research Tools

Description

These MMTV-rtTA mice have expression of the reverse tetracycline-controlled transactivator (rtTA) protein directed primarily to the breast epithelia of the mammary ductal system by the mouse mammary tumor virus (MMTV) promoter. The donating investigator also reports some rtTA expression in salivary glands (particularly in males) as well as prostate glands. These mice are a Tet-On tool that allows conditional, dox-inducible expression of genes primarily in mammary gland epithelial cells and may be useful in studying the endocrine function of mammary tissues and/or breast cancer (for example).

Detailed description

The donating investigator claims homozygous mice are viable and fertile. These MMTV-rtTA mice have expression of the reverse tetracycline-controlled transactivator (rtTA) protein directed primarily to the breast epithelia of the mammary ductal system by the mouse mammary tumor virus (MMTV) promoter. The donating investigator also reports some rtTA expression in salivary glands (particularly in the males) as well as prostate glands. When mated to a mutant strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (TRE, TetRE or tetO), expression of the target gene may be induced with administration of the tetracycline analog doxycycline (dox) in the double mutant offspring. These MMTV-rtTA mice are a Tet-On tool that allows conditional, dox-inducible expression of genes primarily in mammary gland epithelial cells and may be useful in studying the endocrine function of mammary tissues and/or breast cancer (for example).

Development

The MMTV-rtTA-SV40 transgene was designed with the 1.5 kb mouse mammary tumor virus long terminal repeat (MMTV-LTR) promoter, the reverse tetracycline transactivator gene and VP16 activation domain (rtTA-VP16), and SV40 intron/polyA sequence. The rtTA-VP16-polyA sequence is inserted into exon 3 of the rabbit beta-globin gene immediately following the MMTV promoter. This 3.75 kb transgene was injected into one-cell (C57BL/6 x SJL) mouse zygotes. Mice from founder line 4-1 were bred with C57BL/6;SJL wildtype mice to establish the colony. The donating investigator reports that MMTV-rtTA transgenic mice were bred together, to wildtype siblings, and/or to tetO-transgenic mice (on the same mixed C57BL/6;SJL genetic background) for many generations. Mice harboring only the MMTV-rtTA transgene were then sent to The Jackson Laboratory. Upon arrival, transgenic mice were bred with B6SJLF1/J hybrid mice (Stock No. 100012) for at least one generation to establish this colony.

Allele

Symbol: Tg(MMTV-rtTA)4-1Jek
Name: transgene insertion 4-1, Jeffrey E Kudlow
MGI accession ID: MGI:3850385
Generation method: Transgenic
Attributes: Transactivator
Marker symbol: Tg(MMTV-rtTA)4-1Jek
Marker name: transgene insertion 4-1, Jeffrey E Kudlow
Chromosome: UN
Strain of origin: (C57BL/6 x SJL)
Expressed genes: rtTA,reverse tetracycline-controlled transactivator,E. coli
Site of expression: Expression of the reverse tetracycline-controlled transactivator (rtTA) protein is directed primarily to the breast epithelia of the mammary ductal system by the mouse mammary tumor virus (MMTV) promoter. The donating investigator also reports some rtTA expression in salivary glands (particularly in the males) as well as prostate glands
Molecular note: The MMTV-rtTA-SV40 transgene was designed with the 1.5 kb mouse mammary tumor virus long terminal repeat (MMTV-LTR) promoter, the reverse tetracycline transactivator gene and VP16 activation domain (rtTA-VP16), and SV40 intron/polyA sequence. The rtTA-VP16-polyA sequence is inserted into exon 3 of the rabbit beta-globin gene immediately following the MMTV promoter. This 3.75 kb transgene was injected into one-cell (C57BL/6 x SJL) mouse zygotes. Mice from founder line 4-1 were bred with C57BL/6;SJL wildtype mice to establish the colony.