These TetRE-EGFR-tr (TRE-EGFR-tr) transgenic mice express a dominant negative, cytoplasmic tyrosine kinase domain-deleted mutant form of epidermal growth factor receptor (EGFR-tr) regulated by the tetracycline operator (tetO; also called tetracycline-responsive element (TRE, TetRE) or tet-operator) and cytomegalovirus minimal promoter. EGFR-tr expression disrupts subsequent activation of signal transduction pathways that are involved in regulating cellular proliferation, differentiation, and survival. These TetRE-EGFR-tr mice may be bred to generate bi-transgenic mutant mice with conditional (inducible/reversible) expression of a dominant negative EGF receptor, and may be useful in studying receptor tyrosine kinase function of the EGF receptor (ErbB) family proteins and their extracellular protein ligands (including EGF, TGF-α and neuregulins).
Jeffrey Kudlow, University of Alabama at Birmingham
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inducible, Inserted expressed sequence) |
The donating investigator reports that homozygous mice are viable and fertile. These TetRE-EGFR-tr (TRE-EGFR-tr) transgenic mice express a dominant negative, cytoplasmic tyrosine kinase domain-deleted mutant form of epidermal growth factor receptor (EGFR-tr) regulated by the tetracycline operator (tetO; also called tetracycline-responsive element (TRE, TetRE) or tet-operator) and cytomegalovirus minimal promoter. When mated to a mutant strain expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA), tissue EGFR-tr expression may be regulated with the tetracycline analog doxycycline (dox) in the double mutant offspring. EGFR-tr expression disrupts subsequent activation of signal transduction pathways that are involved in regulating cellular proliferation, differentiation, and survival. These TetRE-EGFR-tr mice may be bred to generate bi-transgenic mutant mice with conditional (inducible/reversible) expression of a dominant negative EGF receptor, and may be useful in studying receptor tyrosine kinase function of the EGF receptor (ErbB) family proteins and their extracellular protein ligands (including EGF, TGF-α and neuregulins).
For example, when bred to a strain expressing rtTA in pituitary somatotropes (see Stock No. 010574), this mutant mouse strain may be useful in studies of pituitary development.
When bred to a strain expressing tTA in pituitary lactotropes/mammotropes (see Stock No. 010573 for example), this mutant mouse strain may be useful in studies of pituitary development.
The TetRE-EGFRtr-SV40 transgene was designed with the tetracycline operator (tetO; also called tetracycline-responsive element (TRE, TetRE) or tet-operator) and cytomegalovirus minimal promoter, a 2.3 kb C-terminal truncated mouse epidermal growth factor receptor cDNA sequence (EGFR-tr; encoding amino acid 1-690 followed by a TGA stop codon), and an SV40 intron/polyA sequence. This 4.5 kb transgene was microinjected into one-cell (C57BL/6 x SJL) mouse zygotes. Mice from founder line 2-9 were bred with C57BL/6;SJL wildtype mice to establish the colony. The donating investigator reports that TetRE-EGFR-tr transgenic mice were bred with rtTA-transgenic mice (on the same mixed C57BL/6;SJL genetic background), and then together or to wildtype siblings for many generations. Mice harboring only the TetRE-EGFR-tr transgene were then sent to The Jackson Laboratory. Upon arrival, transgenic mice were bred with B6SJLF1/J hybrid mice (Stock No. 100012) for at least one generation to establish this colony.
Expressed Gene | Egfr, epidermal growth factor receptor, mouse, laboratory |
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Site of Expression |
Allele Name | transgene insertion 2-9, Jeffrey E Kudlow |
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Allele Type | Transgenic (Inducible, Inserted expressed sequence) |
Allele Synonym(s) | TetRE-EGFR-tr; TRE-EGFR-tr |
Gene Symbol and Name | Tg(tetO-Egfr*)2-9Jek, transgene insertion 2-9, Jeffrey E Kudlow |
Gene Synonym(s) | |
Promoter | tetO, tet operator, |
Expressed Gene | Egfr, epidermal growth factor receptor, mouse, laboratory |
Strain of Origin | (C57BL/6 x SJL) |
Chromosome | UN |
Molecular Note | The TetRE-EGFRtr-SV40 transgene was designed with the tetracycline operator (tetO; also called tetracycline-responsive element (TRE, TetRE) or tet-operator) and cytomegalovirus minimal promoter, a 2.3 kb C-terminal truncated mouse epidermal growth factor receptor cDNA sequence (EGFR-tr; encoding amino acid 1-690 followed by a TGA stop codon), and an SV40 intron/polyA sequence. This 4.5 kb transgene was microinjected into one-cell (C57BL/6 x SJL) mouse zygotes. Mice from founder line 2-9 were bred with C57BL/6;SJL wildtype mice to establish the colony. |
Mutations Made By | Jeffrey Kudlow, University of Alabama at Birmingham |
When maintaining a live colony, transgenic carrier mice may be bred with wildtype (noncarrier) siblings. The donating investigator reports that homozygous mice are viable and fertile. B6SJLF1/J mice (Stock No. 100012) are an approximate control.
When using the B6;SJL-Tg(tetO-Egfr*)2-9Jek/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #010575 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non Carrier for Tg(tetO-Egfr*)2-9Jek |
Frozen Mouse Embryo | B6;SJL-Tg(tetO-Egfr*)2-9Jek/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;SJL-Tg(tetO-Egfr*)2-9Jek/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;SJL-Tg(tetO-Egfr*)2-9Jek/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;SJL-Tg(tetO-Egfr*)2-9Jek/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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