These Prl-tTA transgenic mice have expression of the tetracycline-controlled transactivator (tTA) protein directed to PRL-producing cells (pituitary lactotropes/mammotropes) by the rat prolactin (Prl) promoter. The donating investigator also reports some expression in testis. These mice are a Tet-Off tool that allows dox-conditional expression of genes in PRL-producing cells/tissues and may be useful for studying pituitary hormone secretion and proliferation.
Jeffrey Kudlow, University of Alabama at Birmingham
Genetic Background | Generation |
---|---|
|
Allele Type |
---|
Transgenic (Transactivator) |
The donating investigator claims homozygous mice are viable and fertile. These Prl-tTA transgenic mice have expression of the tetracycline-controlled transactivator (tTA) protein directed to PRL-producing cells (pituitary lactotropes/mammotropes) by the rat prolactin (Prl) promoter. The donating investigator also reports some expression in testis. When mated to a mutant strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (TRE, TetRE or tetO), expression of the target gene in the PRL-producing cells may be conditionally abolished with administration of the tetracycline analog doxycycline (dox) in the double mutant offspring. These Prl-tTA transgenic mice are a Tet-Off tool that allows dox-conditional expression of genes in PRL-producing cells/tissues (such as pituitary lactotropes/mammotropes) and may be useful for studying pituitary hormone secretion and proliferation.
The PRL-tTA transgene was designed with a 3.2 kb promoter sequence from the rat prolactin (Prl) gene, a tetracycline-regulated transactivator (tTA) gene, and an SV40 intron/polyA sequence. This 5.2 kb transgene was microinjected into one-cell (C57BL/6 x SJL) mouse zygotes. Mice from founder line 6-5 were bred with C57BL/6;SJL wild-type mice to establish the colony. The donating investigator reports that PRL-tTA transgenic mice were bred together or to tetO-transgenic mice (on the same mixed C57BL/6;SJL genetic background) for many generations. Mice harboring only the PRL-tTA transgene were then sent to The Jackson Laboratory. Upon arrival, transgenic mice were bred with B6SJLF1/J hybrid mice (Stock No. 100012) for at least one generation to establish this colony.
Expressed Gene | tTA, tetracycline-controlled transactivator, E. coli |
---|---|
Site of Expression | expression of the tetracycline-controlled transactivator (tTA) protein is directed to PRL-producing cells (pituitary lactotropes/mammotropes). The donating investigator also reports some expression in testis. |
Allele Name | transgene insertion 6-5, Jeffrey E Kudlow |
---|---|
Allele Type | Transgenic (Transactivator) |
Allele Synonym(s) | Prl-tTA |
Gene Symbol and Name | Tg(Prl-tTA)6-5Jek, transgene insertion 6-5, Jeffrey E Kudlow |
Gene Synonym(s) | |
Promoter | Prl, prolactin, rat |
Expressed Gene | tTA, tetracycline-controlled transactivator, E. coli |
Site of Expression | expression of the tetracycline-controlled transactivator (tTA) protein is directed to PRL-producing cells (pituitary lactotropes/mammotropes). The donating investigator also reports some expression in testis. |
Strain of Origin | (C57BL/6 x SJL) |
Chromosome | UN |
Molecular Note | The PRL-tTA transgene was designed with a 3.2 kb promoter sequence from the rat prolactin (Prl) gene, a tetracycline-regulated transactivator (tTA) gene, and an SV40 intron/polyA sequence. This 5.2 kb transgene was microinjected into one-cell (C57BL/6 x SJL) mouse zygotes. Mice from founder line 6-5 were bred with C57BL/6;SJL wildtype mice to establish the colony. |
Mutations Made By | Jeffrey Kudlow, University of Alabama at Birmingham |
When maintaining a live colony, transgenic carrier mice may be bred with wildtype (noncarrier) siblings. The donating investigator reports that homozygous mice are viable and fertile. B6SJLF1/J mice (Stock No. 100012) are an approximate control.
When using the B6;SJL-Tg(Prl-tTA)6-5Jek/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #010573 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous for Tg(Prl-tTA)6-5Jek |
Frozen Mouse Embryo | B6;SJL-Tg(Prl-tTA)6-5Jek/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;SJL-Tg(Prl-tTA)6-5Jek/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;SJL-Tg(Prl-tTA)6-5Jek/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;SJL-Tg(Prl-tTA)6-5Jek/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.