These mice possess loxP sites on either side of the Pou4f3 (POU domain, class 4, transcription factor 3) coding region followed by an alkaline phosphatase reporter. When crossed with a cre recombinase-expressing strain, the offspring express AP in the retina, dorsal root ganglia, and other cells/tissues.
Jeremy Nathans, Johns Hopkins University
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), Reporter, No functional change) | Pou4f3 | POU domain, class 4, transcription factor 3 |
These mice possess loxP sites on either side of the Pou4f3 (POU domain, class 4, transcription factor 3) coding region followed by an alkaline phosphatase (AP) reporter. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a cre recombinase-expressing strain, the offspring express AP in the retina, dorsal root ganglia, and other cells/tissues.
A loxP site was introduced to the 5' UTR (50 bp upstream of the ATG start codon), and three repeats of the SV40 early terminator were placed in the 3' UTR (300 bp downstream of the stop codon) along with a second loxP site, the human placental alkaline phosphatase (AP) gene, and an FRT-flanked neomycin resistance gene. The targeting vector was introduced to 129-derived embryonic stem cells. The neomycin cassette was excised with a germline FLP recombinase. This line was backcrossed six times to C57BL/6 by the donating laboratory.
Expressed Gene | ALPP, alkaline phosphatase, placental, human |
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Site of Expression |
Allele Name | targeted mutation 1.1, Jeremy Nathans |
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Allele Type | Targeted (Conditional ready (e.g. floxed), Reporter, No functional change) |
Allele Synonym(s) | Brn3c-CKOAP |
Gene Symbol and Name | Pou4f3, POU domain, class 4, transcription factor 3 |
Gene Synonym(s) | |
Expressed Gene | ALPP, alkaline phosphatase, placental, human |
Strain of Origin | 129 |
Chromosome | 18 |
Molecular Note | A loxP site was introduced to the 5' UTR (50 bp upstream of the ATG start codon), and three repeats of the SV40 early terminator were placed in the 3' UTR (300 bp downstream of the stop codon) along with a second loxP site, the human placental alkaline phosphatase (AP) gene, and an FRT-flanked neomycin resistance gene. The targeting vector was introduced to 129-derived embryonic stem cells. The neomycin cassette was excised with a germline FLP recombinase. |
Mutations Made By | Jeremy Nathans, Johns Hopkins University |
When maintained as a live colony, heterozygotes or homozygotes may be bred.
When using the B6.129-Pou4f3tm1.1Nat/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #010560 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Pou4f3<tm1.1Nat> |
Frozen Mouse Embryo | B6.129-Pou4f3<tm1.1Nat>/J | $2595.00 |
Frozen Mouse Embryo | B6.129-Pou4f3<tm1.1Nat>/J | $2595.00 |
Frozen Mouse Embryo | B6.129-Pou4f3<tm1.1Nat>/J | $3373.50 |
Frozen Mouse Embryo | B6.129-Pou4f3<tm1.1Nat>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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