These R26rtTACreER mice have a CreERT, reverse tetracycline trans-activator (rtTA), and Tet-responsive element (TRE) targeted to the mouse Gt(ROSA)26Sor gene to create a gene expression tool with two independent layers of pharmacologic control and a widespread pattern of activity. In this construct, expression of tamoxifen-sensitive CreERT is under the control of rtTA. Upon administration of doxycycline, rtTA activates transcription of the CreER coding region. This enhances the dynamic range of CreER and enables sparse labeling in situations where reduced recombination efficiency facilitates behavioral, morphological or functional studies of modified cells among populations of unmodified neighbors.
Jeremy Nathans, Johns Hopkins University
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Recombinase-expressing, Inducible, Transactivator) | Gt(ROSA)26Sor | gene trap ROSA 26, Philippe Soriano |
These R26rtTACreER mice have a CreERT, reverse tetracycline trans-activator (rtTA), and Tet-responsive element (TRE) targeted to the mouse Gt(ROSA)26Sor gene to create a gene expression tool with two independent layers of pharmacologic control and a widespread pattern of activity. In this construct, expression of tamoxifen-sensitive CreERT is under the control of rtTA, a sequence-specific DNA-binding protein that activates transcription upon binding tetracycline derivatives such as doxycycline. Upon administration of doxycycline, rtTA activates transcription of the CreER coding region by binding to the Tet operator located immediately 5' of a minimal promoter. This enhances the dynamic range of CreER and enables sparse labeling in situations where reduced recombination efficiency facilitates behavioral, morphological or functional studies of modified cells among populations of unmodified neighbors.
A splice acceptor, reverse tetracycline-responsive transcriptional activator (rtTA), six repeats of an SV40 transcriptional terminator, tetracycline response element (TRE) enhancer-promoter, CreER fusion protein open reading frame, bovine growth hormone 3' UTR and transcriptional terminator region were introuduced to exon 1 of the targeted gene. The vector was introduced to 129-derived embryonic stem cells. This line was backcrossed 3 times to C57BL/6 by the donating laboratory.
Expressed Gene | rtTA, reverse tetracycline-controlled transactivator, E. coli |
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Expressed Gene | cre/ER, cre inducible estrogen receptor, bacteriophage P1/Human |
Site of Expression | widespread, doxycycline inducible expression of Cre recombinase |
Allele Name | targeted mutation 3, Jeremy Nathans |
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Allele Type | Targeted (Recombinase-expressing, Inducible, Transactivator) |
Allele Synonym(s) | R26rtTACreER |
Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano |
Gene Synonym(s) | |
Promoter | tetO, tet operator, |
Expressed Gene | rtTA, reverse tetracycline-controlled transactivator, E. coli |
Expressed Gene | cre/ER, cre inducible estrogen receptor, bacteriophage P1/Human |
Site of Expression | widespread, doxycycline inducible expression of Cre recombinase |
Strain of Origin | 129 |
Chromosome | 6 |
Molecular Note | The construct consists of the rtTA coding region preceded by a splice acceptor so that it can be spliced onto the 5' exon (exon1) of the endogenous ROSA26 transcript, and is followed by six repeats of an SV40 transcriptional terminator signal ("stop"). In the presence of doxycycline, the rtTA protein activates transcription from the seven head-to-tail Tet response elements immediately 5' of a minimal cytomegalovirus immediate early promoter (TRE) of the CreER fusion protein open reading frame (followed by the bovine growth hormone 3' UTR and transcriptional terminator region), leading to expression of CreER. |
Mutations Made By | Jeremy Nathans, Johns Hopkins University |
When maintained as a live colony, homozygotes or heterozygotes may be bred.
When using the B6;129-Gt(ROSA)26Sortm3(rtTA,tetO-cre/ERT)Nat/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #010557 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Gt(ROSA)26Sor<tm2Nat> |
Frozen Mouse Embryo | B6;129-Gt(ROSA)26Sor<tm3(rtTA tetO-cre/ERT)Nat>/J Frozen Emb | $2595.00 |
Frozen Mouse Embryo | B6;129-Gt(ROSA)26Sor<tm3(rtTA tetO-cre/ERT)Nat>/J Frozen Emb | $2595.00 |
Frozen Mouse Embryo | B6;129-Gt(ROSA)26Sor<tm3(rtTA tetO-cre/ERT)Nat>/J Frozen Emb | $3373.50 |
Frozen Mouse Embryo | B6;129-Gt(ROSA)26Sor<tm3(rtTA tetO-cre/ERT)Nat>/J Frozen Emb | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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