STOCK Pax7^tm1(cre)Mrc/J

Stock number: 010530
Product name: Pax7-Cre
Research areas: Research Tools

Description

Removal of this mouse colony is imminent. If live mice are needed for your studies, it is advised that they be ordered immediately. After removal, the mice will be available from a cryorecovery.

This Pax7 knock-in strain may be useful in studies of alveolar rhabdomyosarcoma and embryonic myogenesis.

Detailed description

In this targeted mutation strain, the mouse paired box gene 7 (Pax7) promoter drives expression of cre in progenitor cells of skeletal muscle lineage and satelllite cells. Crossing this mouse with another carrying a loxP-flanked genomic segment of interest enables tissue-specific excision of the floxed segment in the offspring. Normal gene expression is not affected.

Development

An IRES-cre-Frt-neo-Frt cassette was introduced to the 3' UTR of the targeted gene following the stop codon in exon 10. The (129X1/SvJ x 129S1/Sv)F1- Kitl⁺-derived R1 embryonic stem (ES) cell line was used to create the mutation. This strain was maintained on a mixed C57BL/6 and 129 genetic background by the donating laboratory. Genotype testing at The Jackson Laboratory indicates that the neomycin portion of the insertion has been excised.

Allele

Symbol: Pax7^tm1(cre)Mrc
Name: targeted mutation 1, Mario R Capecchi
MGI accession ID: MGI:3497712
Generation method: Targeted
Attributes: Recombinase-expressing
Marker symbol: Pax7
Marker name: paired box 7
Chromosome: 4
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: cre is expressed in progenitor cells of skeletal muscle lineage and satellite cells.
Molecular note: An IRES-Cre-FRT-neo-FRT cassette was inserted into the 3' UTR following the stop codon in exon 10. Crossing with mice expressing FLP-e excised the neo. The insertion did not interfere with normal Pax7 function.