STOCK Pax7^tm1(cre)Mrc/J

Stock number: 010530
Product name: Pax7-Cre
Research areas: Research Tools

Description

This Pax7 knock-in strain may be useful in studies of alveolar rhabdomyosarcoma and embryonic myogenesis.

Detailed description

In this targeted mutation strain, the mouse paired box gene 7 (Pax7) promoter drives expression of cre in progenitor cells of skeletal muscle lineage and satelllite cells. Crossing this mouse with another carrying a loxP-flanked genomic segment of interest enables tissue-specific excision of the floxed segment in the offspring. Normal gene expression is not affected.

Development

An IRES-cre-Frt-neo-Frt cassette was introduced to the 3' UTR of the targeted gene following the stop codon in exon 10. The (129X1/SvJ x 129S1/Sv)F1- Kitl⁺-derived R1 embryonic stem (ES) cell line was used to create the mutation. This strain was maintained on a mixed C57BL/6 and 129 genetic background by the donating laboratory. Genotype testing at The Jackson Laboratory indicates that the neomycin portion of the insertion has been excised.

Allele

Symbol: Pax7^tm1(cre)Mrc
Name: targeted mutation 1, Mario R Capecchi
MGI accession ID: MGI:3497712
Generation method: Targeted
Attributes: Recombinase-expressing
Synonyms: Pax7-cre; Pax7^ICNm
Marker symbol: Pax7
Marker name: paired box 7
Chromosome: 4
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: cre is expressed in progenitor cells of skeletal muscle lineage and satellite cells.
Molecular note: An IRES-Cre-FRT-neo-FRT cassette was inserted into the 3' UTR following the stop codon in exon 10. Crossing with mice expressing FLP-e excised the neo. The insertion did not interfere with normal Pax7 function.