This Pax7 knock-in strain may be useful in studies of alveolar rhabdomyosarcoma and embryonic myogenesis.
Dr. Mario R Capecchi, University of Utah
Genetic Background | Generation |
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?+F18
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Recombinase-expressing) | Pax7 | paired box 7 |
In this targeted mutation strain, the mouse paired box gene 7 (Pax7) promoter drives expression of cre in progenitor cells of skeletal muscle lineage and satelllite cells. Crossing this mouse with another carrying a loxP-flanked genomic segment of interest enables tissue-specific excision of the floxed segment in the offspring. Normal gene expression is not affected.
An IRES-cre-Frt-neo-Frt cassette was introduced to the 3' UTR of the targeted gene following the stop codon in exon 10. The (129X1/SvJ x 129S1/Sv)F1- Kitl+-derived R1 embryonic stem (ES) cell line was used to create the mutation. This strain was maintained on a mixed C57BL/6 and 129 genetic background by the donating laboratory. Genotype testing at The Jackson Laboratory indicates that the neomycin portion of the insertion has been excised.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | cre is expressed in progenitor cells of skeletal muscle lineage and satellite cells. |
Allele Name | targeted mutation 1, Mario R Capecchi |
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Allele Type | Targeted (Recombinase-expressing) |
Allele Synonym(s) | Pax7ICNm; Pax7-cre |
Gene Symbol and Name | Pax7, paired box 7 |
Gene Synonym(s) | |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | cre is expressed in progenitor cells of skeletal muscle lineage and satellite cells. |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 4 |
Molecular Note | An IRES-Cre-FRT-neo-FRT cassette was inserted into the 3' UTR following the stop codon in exon 10. Crossing with mice expressing FLP-e excised the neo. The insertion did not interfere with normal Pax7 function. |
Mutations Made By | Dr. Mario Capecchi, University of Utah |
When maintained in live colonies, homozygotes or heterozygotes may be bred.
When using the Pax7-Cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #010530 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous for Pax7<tm1(cre)Mrc> |
Frozen Mouse Embryo | STOCK Pax7<tm1(cre)Mrc>/J | $2595.00 |
Frozen Mouse Embryo | STOCK Pax7<tm1(cre)Mrc>/J | $2595.00 |
Frozen Mouse Embryo | STOCK Pax7<tm1(cre)Mrc>/J | $3373.50 |
Frozen Mouse Embryo | STOCK Pax7<tm1(cre)Mrc>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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