These Ly-49A transgenic mice express the mouse Klra1 (killer cell lectin-like receptor, subfamily A, member 1) gene under the control of a modified mouse CD4 antigen (Cd4) promoter (this promoter lacks the silencer element sequence suppressing expression in CD8 T cells). Transgene expression is detected during early T cell development and throughout CD4 and CD8 T cell differentiation and migration. This mutant mouse strain may be useful in studies of autoimmune and inflammatory diseases.
Mary Pauza, Southern Illinois University
Genetic Background | Generation |
---|---|
|
Allele Type |
---|
Transgenic (Inserted expressed sequence) |
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Spontaneous | H2 | histocompatibility-2, MHC |
These Ly-49A transgenic mice express the mouse Klra1 (killer cell lectin-like receptor, subfamily A, member 1) gene under the control of a modified mouse CD4 antigen (Cd4) promoter (this promoter lacks the silencer element sequence suppressing expression in CD8 T cells). Transgene expression is detected during early T cell development and throughout CD4 and CD8 T cell differentiation and migration. On the B10.HTG background, which lacks H2dd, cell surface transgene expression is increased when compared to transgenic mice on backgrounds carrying H2dd. The presence of H2dd ligand during T cell development impairs negative selection in the thymus and can result in increased apoptosis of thymocytes, fewer CD4 single positive cells, and autoreactive T cells with the development of a graft-versus-host-disease-like syndrome. This mutant mouse strain may be useful in studies of autoimmune and inflammatory diseases.
The Ly-49A transgenic construct containing the mouse Klra1 (killer cell lectin-like receptor, subfamily A, member 1) gene under the control of a modified mouse CD4 antigen (Cd4) promoter (this promoter lacks the silencer element sequence suppressing expression in CD8 T cells) was injected into fertilized C57BL/6 mouse eggs. Founder line 6295 was established. The mice were then crossed to B10.HTG mice for at least 10 generations. These mice carry the H2g allele.
Expressed Gene | Klra1, killer cell lectin-like receptor, subfamily A, member 1, mouse, laboratory |
---|---|
Site of Expression | |
Site of Expression |
Allele Name | transgene insertion 6295, David Lo |
---|---|
Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | Ly-49A; Tg(Cd4-Klra1)1Dl |
Gene Symbol and Name | Tg(Cd4-Klra1)6295Dl, transgene insertion 6295, David Lo |
Gene Synonym(s) | |
Promoter | Cd4, CD4 antigen, mouse, laboratory |
Expressed Gene | Klra1, killer cell lectin-like receptor, subfamily A, member 1, mouse, laboratory |
Strain of Origin | C57BL/6 |
Chromosome | UN |
Molecular Note | The Ly-49A transgenic construct containing the mouse Klra1 (killer cell lectin-like receptor, subfamily A, member 1) gene under the control of a modified mouse CD4 antigen (Cd4) promoter (this promoter lacks the silencer element sequence suppressing expression in CD8 T cells) was injected into fertilized C57BL/6 mouse eggs. Founder line 6295 was established. |
Mutations Made By | Mary Pauza, Southern Illinois University |
When maintaining a live colony, these mice can be bred as hemizygotes. To cryorecover this strain, the oocyte donor strain (Stock No. 000999 or 001012) must also be cryorecovered to preserve the genetic background of the transgenic strain.
When using the B10.Cg-H2g Tg(Cd4-Klra1)6295Dl/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #010514 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous or Non carrier for Tg(Cd4-Klra1)6295Dl |
Frozen Mouse Embryo | B10.Cg-H2<g> Tg(Cd4-Klra1)6295Dl/J | $2595.00 |
Frozen Mouse Embryo | B10.Cg-H2<g> Tg(Cd4-Klra1)6295Dl/J | $2595.00 |
Frozen Mouse Embryo | B10.Cg-H2<g> Tg(Cd4-Klra1)6295Dl/J | $3373.50 |
Frozen Mouse Embryo | B10.Cg-H2<g> Tg(Cd4-Klra1)6295Dl/J | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.