This targeted mutation of the integrin alpha 4 (Itga4) gene exhibits impaired lymphocyte transmigration and homing to the gut. This mutant mouse strain may be useful in studies of leukocyte migration and homing.
Motomu Shimaoka, Immune Disease Institute (formerly CBRI)
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Not Applicable) | Itga4 | integrin alpha 4 |
Mice homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Lymphocytes exhibit increased basal adhesiveness to VCAM1 and MAdCAM1 under physiological conditions as well as reduced transmigration across VCAM1 and MAdCAM1 substrates and endothelial monolayers under shear stress. Homing of mutant lymphocytes to the gut is reduced by approximately 50%. In addition, fewer lymphocytes are found in the gut and Peyer's Patches are smaller in size than wild-type. This mutant mouse strain may be useful in studies of leukocyte migration and homing.
A targeting vector was designed to contain exons 26 and 27, an engineered Xba1 site, an ACN cassette, and exon 28, respectively. Exon 28 contains nucleotide mutations to generate an amino acid substitution of alanine for arginine. The loxP-flanked ACN cassette contains the neomycin resistance gene and Cre recombinase gene under the control of the sperm-specific angiotensin-converting enzyme promoter. Cre-mediated recombination during spermatogenesis of chimeric males removes the cassette leaving a single loxP- site between exons 27 and 28. The construct was electroporated into C57BL/6-derived Bruce-4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into BALB/c blastocysts, and the resulting chimeric males were crossed to C57BL/6 females. Homozygotes were generated and then maintained by intercross prior to arrival at The Jackson Laboratory.
Allele Name | targeted mutation 1, Motomu Shimaoka |
---|---|
Allele Type | Targeted (Not Applicable) |
Allele Synonym(s) | Itga4-R/AGFFKR |
Gene Symbol and Name | Itga4, integrin alpha 4 |
Gene Synonym(s) | |
Promoter | Itga4, integrin alpha 4, mouse, laboratory |
Strain of Origin | B6.Cg-Thy1a |
Chromosome | 2 |
Molecular Note | Exon 28 was replaced with one in which nucleotide substitutions resulted in amino acid substitution of GFFKA for GFFKR. This sequence is involved in the formation of a membrane-proximal salt bridge necessary for inactivation. A self-excising neo cassette was inserted upstream of exon 28 and subsequently deleted in chimeras. |
Mutations Made By | Motomu Shimaoka, Immune Disease Institute (formerly CBRI) |
When maintaining a live colony, these mice are bred as homozygotes.
When using the C57BL/6-Itga4tm1Mshi/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #010501 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Itga4<tm1Mshi> |
Frozen Mouse Embryo | C57BL/6-Itga4<tm1Mshi>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Itga4<tm1Mshi>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Itga4<tm1Mshi>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | C57BL/6-Itga4<tm1Mshi>/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.