Mice heterozygous for this mutation in the budding uninhibited by benzimidazoles 1 homolog (Bub1b) gene exhibit splenomegaly, an increase in extramedullary megakaryocytes and reduced numbers of peripheral red blood cells. This mutant mouse strain may be useful in studies of early embryonic development, hematopoiesis, megakaryopoiesis, tumorigenesis and genomic instability.
Wei Dai, New York University Langone Medical Center
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Gene trapped (Reporter, Null/Knockout) | Bub1b | BUB1B, mitotic checkpoint serine/threonine kinase |
Mice heterozygous for this mutation exhibit splenomegaly accompanied by a loss of marginal zone boundaries and an increase in mature megakaryocytes. Approximately 50% of mice have significantly reduced numbers of peripheral red blood cells, however platelet numbers are not increased. Homozygous embryos do not survive beyond early gestation (E8.5) due to impaired blastocyst proliferation and extensive apoptosis. Mouse embryonic fibroblasts from heterozygous mice exhibit defective spindle checkpoint activation, an important mechanism for genomic stability. In addition, mice are susceptible to development of lung and colon adenocarcinomas following challenge with carcinogen. Mice homozygous for the mutation are embryonic lethal. This mutant mouse strain may be useful in studies of early embryonic development, hematopoiesis, megakaryopoiesis tumorigenesis and genomic instability.
A retroviral genetrap vector with a splice acceptor fused to neo with a poly A signal and a PGK promoter fused to the coding sequence of the BTK gene with a splice donor site was used to disrupt intron 1 between exon 1 and 2 of the endogenous gene. The construct was electroporated into 129-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Heterozygous offspring were crossed to C57BL/6 and then maintained by intercross.
Allele Name | gene trap 1, Wei Dai |
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Allele Type | Gene trapped (Reporter, Null/Knockout) |
Allele Synonym(s) | BUBR1- |
Gene Symbol and Name | Bub1b, BUB1B, mitotic checkpoint serine/threonine kinase |
Gene Synonym(s) | |
Strain of Origin | 129S4/SvJae |
Chromosome | 2 |
Molecular Note | The endogenous gene was disrupted by the integration of a gene trap vector into intron 1. The gene trap vector contained a splice acceptor, neo, a polyadenylation signal, PGK-btk, and a a splice donor. |
Mutations Made By | Wei Dai, New York University Langone Medical Center |
When maintaining a live colony, these mice are bred as heterozygotes. Mice homozygous for the mutation are embryonic lethal.
When using the B6;129-Bub1bGt(neo-btk)1Dai/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #010495 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild type for Bub1b<Gt(neo-btk)1Dai> |
Frozen Mouse Embryo | B6;129-Bub1b<Gt(neo-btk)1Dai>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129-Bub1b<Gt(neo-btk)1Dai>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129-Bub1b<Gt(neo-btk)1Dai>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129-Bub1b<Gt(neo-btk)1Dai>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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